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AB91343

Anti-Pol V antibody

5

(1 Review)

|

(2 Publications)

Rabbit Polyclonal Pol V antibody. Suitable for WB and reacts with Escherichia coli samples. Cited in 2 publications.

View Alternative Names

b1183, JW1172, umuD, Protein UmuD, DNA polymerase V, Pol V

1 Images
Western blot - Anti-Pol V antibody (AB91343)
  • WB

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Western blot - Anti-Pol V antibody (AB91343)

All lanes:

Western blot - Anti-Pol V antibody (ab91343) at 1/3000 dilution

Lane 1:

protein extracts from E. coli DE274 (lexA51, recA730)

Lane 2:

protein extract from E. coli DE274 (lexA51, recA730) treated with mitomycin C

Predicted band size: 14 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Escherichia coli

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000", "WB-species-notes": "<p>Predicted molecular weight: 14 kDa (processed form. Precursor form is 17 kDa).</p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 6 - 8.5 Preservative: 0.05% Sodium azide Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pol V alternately known as DNA polymerase V is a specialized DNA polymerase involved in translesion DNA synthesis in prokaryotes. It has an important role in bypassing DNA damage during replication. The Pol V complex comprises a heterotrimer of UmuD′2C proteins and has a molecular mass of approximately 140 kDa. It is expressed in bacterial cells particularly Escherichia coli where it participates in the SOS response to cellular stress and damage.
Biological function summary

In bacterial systems undergoing stress Pol V addresses DNA lesions that could otherwise stall replication forks. As part of the SOS response it operates together with the RecA protein to mediate error-prone DNA repair. This activity is part of the larger UmuDC complex which allows cells to survive DNA damage by incorporating nucleotides opposite DNA lesions albeit at the cost of increased mutagenesis.

Pathways

Pol V functions in the DNA damage tolerance pathway specifically targeting translesion synthesis. It cooperates mainly with RecA in this pathway to facilitate its polymerase activity. Pol V's ability to synthesize DNA across lesions is a critical component of bacterial DNA damage tolerance ensuring continuity of replication even when template damage is present.

Pol V holds indirect relevance primarily in bacterial pathogenicity. Increased mutation rates mediated by Pol V can contribute to the development of antibiotic resistance in bacteria. Through the SOS response including the action of RecA with Pol V stress-induced genotoxic conditions in bacteria can lead to adaptive mutations potentially leading to resistant strains. Studying Pol V provides insight into mechanisms of bacterial DNA repair and mutation that are significant in microbial genetics and antibiotic resistance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in UV protection and mutation. Poorly processive, error-prone DNA polymerase involved in translesion repair (PubMed : 10801133). Essential for induced (or SOS) mutagenesis. Able to replicate DNA across DNA lesions (thymine photodimers and abasic sites, called translesion synthesis) in the presence of activated RecA; efficiency is maximal in the presence of the beta sliding-clamp and clamp-loading complex of DNA polymerase III plus single-stranded binding protein (SSB) (PubMed : 10801133). RecA and to a lesser extent the beta clamp-complex may target Pol V to replication complexes stalled at DNA template lesions (PubMed : 10801133).
See full target information umuD
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of 111:5526-31 PubMed24706928

2014

Chronology in lesion tolerance gives priority to genetic variability.

Applications

Unspecified application

Species

Unspecified reactive species

Karel Naiman,Gaëlle Philippin,Robert P Fuchs,Vincent Pagès

Journal of bacteriology 195:1179-93 PubMed23292773

2013

A single residue unique to DinB-like proteins limits formation of the polymerase IV multiprotein complex in Escherichia coli.

Applications

Unspecified application

Species

Escherichia coli

Tiziana M Cafarelli,Thomas J Rands,Ryan W Benson,Pamela A Rudnicki,Ida Lin,Veronica G Godoy
View all publications
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