Anti-Pol V antibody

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Pol V alternately known as DNA polymerase V is a specialized DNA polymerase involved in translesion DNA synthesis in prokaryotes. It has an important role in bypassing DNA damage during replication. The Pol V complex comprises a heterotrimer of UmuD′2C proteins and has a molecular mass of approximately 140 kDa. It is expressed in bacterial cells particularly Escherichia coli where it participates in the SOS response to cellular stress and damage.

Biological function summary

In bacterial systems undergoing stress Pol V addresses DNA lesions that could otherwise stall replication forks. As part of the SOS response it operates together with the RecA protein to mediate error-prone DNA repair. This activity is part of the larger UmuDC complex which allows cells to survive DNA damage by incorporating nucleotides opposite DNA lesions albeit at the cost of increased mutagenesis.

Pathways

Pol V functions in the DNA damage tolerance pathway specifically targeting translesion synthesis. It cooperates mainly with RecA in this pathway to facilitate its polymerase activity. Pol V's ability to synthesize DNA across lesions is a critical component of bacterial DNA damage tolerance ensuring continuity of replication even when template damage is present.

Associated diseases and disorders

Pol V holds indirect relevance primarily in bacterial pathogenicity. Increased mutation rates mediated by Pol V can contribute to the development of antibiotic resistance in bacteria. Through the SOS response including the action of RecA with Pol V stress-induced genotoxic conditions in bacteria can lead to adaptive mutations potentially leading to resistant strains. Studying Pol V provides insight into mechanisms of bacterial DNA repair and mutation that are significant in microbial genetics and antibiotic resistance.