JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB103591

Anti-POLA2 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal POLA2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human POLA2.

View Alternative Names

DNA polymerase alpha subunit B, DNA polymerase alpha 70 kDa subunit, POLA2

2 Images
Western blot - Anti-POLA2 antibody (AB103591)
  • WB

Unknown

Western blot - Anti-POLA2 antibody (AB103591)

All lanes:

Western blot - Anti-POLA2 antibody (ab103591) at 1/500 dilution

All lanes:

Human placenta tissue lysate at 50 µg

Predicted band size: 66 kDa

false

Western blot - Anti-POLA2 antibody (AB103591)
  • WB

Unknown

Western blot - Anti-POLA2 antibody (AB103591)

All lanes:

Western blot - Anti-POLA2 antibody (ab103591) at 1/500 dilution

Lane 1:

POLA2 transfected 293T cell line lysate at 25 µg

Lane 2:

Non-transfected 293T cell line lysate at 25 µg

Predicted band size: 66 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human POLA2.

Q14181

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

POLA2 also known as DNA polymerase alpha-2 subunit or p70 functions mechanically as a part of the DNA polymerase alpha complex. This protein has a mass of approximately 70 kDa. It plays a role in the initiation of DNA replication by synthesizing short RNA-DNA hybrid primers during the early phase. POLA2 expresses widely with prominent presence in proliferating tissues due to its role in cell division.
Biological function summary

POLA2 participates in the assembly of the DNA polymerase alpha complex which is a critical component in the inception of DNA replication. This complex comprises multiple subunits including the catalytic POLA1 subunit with POLA2 acting as a regulatory subunit that stabilizes the complex and contributes to its activity. POLA2 is necessary for ensuring genomic integrity as the complex initiates DNA synthesis upon the replication fork.

Pathways

POLA2 is integral to DNA replication and cell cycle regulation. It facilitates replication initiation anchoring the complex to the DNA template which coordinates with proteins like primase to synthesize the initial RNA-DNA segments. This involvement links POLA2 to the broader cell division cycle pathway interacting functionally alongside other participant proteins such as MCMs and CDC45 that orchestrate the progression through replication.

POLA2 has associations with cancer reflecting its role in cell proliferation. Overexpression or dysregulation of POLA2 can lead to uncontrolled cell division contributing to tumorigenesis. Studies also connect POLA2 to certain genetic disorders involving replication stress. Defects in POLA2 have correlations to replication-based diseases and show interaction with other replication proteins like ATR whose dysfunction can exacerbate replication errors leading to disease phenotypes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis (PubMed : 9705292). During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity). The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (By similarity). These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity).
See full target information POLA2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 12: PubMed32933200

2020

DNA Polymerase Alpha Subunit B Is a Binding Protein for Erlotinib Resistance in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tae Young Kim,Eun Sun Ji,Ju Yeon Lee,Jin Young Kim,Jong Shin Yoo,A Marcell Szasz,Balazs Dome,Gyorgy Marko-Varga,Ho Jeong Kwon
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB26721

Anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade (AB26721)

  • 4
  • 12
Primary Antibodies

AB314638

Anti-ZC3H13 antibody [EPR25077-7]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZC3H13 antibody [EPR25077-7] (AB314638)

  • 0
  • 0
Primary Antibodies

AB200376

Anti-Zinc finger MIZ domain-containing protein 1 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc finger MIZ domain-containing protein 1 antibody - N-terminal (AB200376)

  • 0
  • 0
Primary Antibodies

AB191536

Anti-PAG608 antibody

Western blot - Anti-PAG608 antibody (AB191536)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com