Anti-POLD1 antibody [EPR15118] (ab186407) is a rabbit monoclonal antibody that is used to detect POLD1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 10 publications
Images
![Western blot - Anti-POLD1 antibody [EPR15118] (AB186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--western-blot-img104803.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-POLD1 antibody [EPR15118] (AB186407)")
![Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (AB186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--immunocytochemistry-immunofluorescence-img17675.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (AB186407)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (AB186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110997.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (AB186407)")
![Flow Cytometry (Intracellular) - Anti-POLD1 antibody [EPR15118] (AB186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--flow-cytometry-intracellular-img37734.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-POLD1 antibody [EPR15118] (AB186407)")
![Western blot - Anti-POLD1 antibody [EPR15118] (AB186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--western-blot-img106459.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-POLD1 antibody [EPR15118] (AB186407)")
Publications
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- Cell reports 42:1120622023GEN1 promotes common fragile site expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnaid Benitez,Marie Sebald,Radhakrishnan Kanagaraj,Monica C Rodrigo-Brenni,Ying Wai Chan,Chih-Chao Liang,Stephen C WestPubMed 36729836
- Frontiers in molecular biosciences 10:11199002023Unraveling the impact of a germline heterozygous frameshift variant in serrated polyposis syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaia Bonjoch,Yasmin Soares de Lima,Marcos Díaz-Gay,Isabella Dotti,Jenifer Muñoz,Leticia Moreira,Sabela Carballal,Teresa Ocaña,Miriam Cuatrecasas,Oswaldo Ortiz,Antoni Castells,Maria Pellisé,Francesc Balaguer,Azucena Salas,Ludmil B Alexandrov,Sergi Castellví-BelPubMed 36756361
- Nature communications 13:60902022Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHana Polasek-Sedlackova,Thomas C R Miller,Jana Krejci,Maj-Britt Rask,Jiri LukasPubMed 36241664
- European journal of medical genetics 64:1043332021Child to adulthood clinical description of MDPL syndrome due to a novel variant in POLD1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBattisti Gladys,Wintjens René,Decottignies Anabelle,Merhi Ahmad,Fervaille Caroline,Sokal Etienne,Karadurmus Deniz,Benoit Valerie,Claessens Anick,Martinet Jean-Paul,Martiat Benoît,Kinzinger Philippe,Maystadt IsabellePubMed 34517090
- Frontiers in cell and developmental biology 9:6185862021CTCF Mediates Replicative Senescence Through POLD1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuli Hou,Qiao Song,Shichao Gao,Xiaomin Zhang,Yaqi Wang,Jing Liu,Jingxuan Fu,Min Cao,Peichang WangPubMed 33692996
- Molecular cell 80:682-698.e72020Hyperactive CDK2 Activity in Basal-like Breast Cancer Imposes a Genome Integrity Liability that Can Be Exploited by Targeting DNA Polymerase ε.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVladislav O Sviderskiy,Lili Blumenberg,Elizabeth Gorodetsky,Triantafyllia R Karakousi,Nicole Hirsh,Samantha W Alvarez,Erdem M Terzi,Efiyenia Kaparos,Gabrielle C Whiten,Shakirah Ssebyala,Peter Tonzi,Hannan Mir,Benjamin G Neel,Tony T Huang,Sylvia Adams,Kelly V Ruggles,Richard PossematoPubMed 33152268
- Endocrine connections 9:923-9322020POLE and POLD1 pathogenic variants in the proofreading domain in papillary thyroid cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbdul K Siraj,Rong Bu,Maham Arshad,Kaleem Iqbal,Sandeep Kumar Parvathareddy,Tariq Masoodi,Laila Omar Ghazwani,Saif S Al-Sobhi,Fouad Al-Dayel,Khawla S Al-KurayaPubMed 32992294
- Molecular genetics & genomic medicine 8:e12802020Preliminary study on the function of the POLD1 (CDC2) EXON2 c.56G>A mutation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Liu et. al.PubMed 32432416
- Cancer cell international 19:3342019Germline and proofreading domain mutations in endometrial carcinoma from Middle Eastern region.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbdul K Siraj et. al.PubMed 31866764
- Gastroenterology 157:1398-1412.e92019Fibrogenic Activity of MECP2 Is Regulated by Phosphorylation in Hepatic Stellate Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEva Moran-Salvador et. al.PubMed 31352003
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected | Expected |
| Rat | Tested | Expected | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24022480, PubMed:24035200, PubMed:31449058). Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24022480, PubMed:24035200). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites (PubMed:19074196, PubMed:24191025).
Alternative names
POLD, POLD1, DNA polymerase delta catalytic subunit, 3'-5' exodeoxyribonuclease, DNA polymerase subunit delta p125
Recommended products
Anti-POLD1 antibody [EPR15118] (ab186407) is a rabbit monoclonal antibody that is used to detect POLD1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 10 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15118
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-POLD1 antibody [EPR15118] (ab186407) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.
What is the molecular weight of POLD1?
Anti-POLD1 [EPR15118] (ab186407) specifically detects a band for POLD1 (UniProt: P28340) at a molecular weight of 124kDa.
Recommended positive controls
MOLT4, HeLa, Jurkat, K562, C6, Raw264.7 and PC12 cell lysates; Human cervix carcinoma and colon tissues; HeLa and K562 cells.
Trusted by the scientific community
Anti-POLD1 [EPR15118] (ab186407) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products
We have a range of other formats of antibody clone [EPR15118] also available for your convenience:
ab186407, Carrier free - Anti-POLD1 antibody [EPR15118] - BSA and Azide free ab240300, PE - PE Anti-DNA Polymerase delta, catalytic subunit antibody [EPR15118] ab306081, APC - APC Anti-DNA Polymerase delta, catalytic subunit antibody [EPR15118] ab306082, HRP - HRP Anti-DNA Polymerase delta, catalytic subunit antibody [EPR15118] ab306083, Alkaline Phosphatase - AP Anti-DNA Polymerase delta, catalytic subunit antibody [EPR15118] ab308862, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-DNA Polymerase delta, catalytic subunit antibody [EPR15118] ab309788, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-DNA Polymerase delta, catalytic subunit antibody [EPR15118] ab310157, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-DNA Polymerase delta, catalytic subunit antibody [EPR15118] ab310588, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-DNA Polymerase delta, catalytic subunit antibody [EPR15118] ab312118, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-POLD1 antibody [EPR15118] ab312602, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-POLD1 antibody [EPR15118] ab321298
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
POLD1 also known as DNA polymerase delta is a catalytic subunit of the DNA polymerase delta complex with alternative names such as delta 647 delta 555 delta 488 delta APC delta ALP and ADN-594. Its mass is approximately 124 kDa. POLD1 is expressed in various tissues but shows high expression in proliferating cells. The primary role is to facilitate the high-fidelity DNA replication process by synthesizing the lagging strand during DNA replication and repair. Its enzymatic activity involves adding nucleotides to a primer strand of DNA and executing proofreading functions to ensure replication accuracy.
- Biological function summary
POLD1 is integral to the replication machinery where it functions as part of the multi-subunit DNA polymerase delta complex. This complex plays a pivotal role in promoting genome stability by participating in DNA synthesis proofreading and repair processes. The complex provides both polymerase and exonuclease activity ensuring accuracy during DNA replication. Besides POLD1 interacts with PCNA (proliferating cell nuclear antigen) to enhance its enzymatic efficiency during DNA synthesis.
- Pathways
POLD1 is involved in the DNA replication and repair pathways. Its interaction with replication protein A (RPA) and PCNA facilitates the smooth completion of Okazaki fragment processing within the DNA replication pathway. Additionally POLD1 plays a role in the base excision repair pathway working alongside other repair proteins such as FEN1 (flap endonuclease 1) and LIG1 (DNA ligase I) to correct DNA lesions. These interactions enforce fidelity and speed during genetic information transfer.
- Associated diseases and disorders
Mutations or dysregulation of POLD1 have links to various cancers and genomic instability disorders. Somatic mutations in POLD1 are associated with colorectal cancer where the impaired replication and repair capabilities lead to accumulated mutations. Another disorder related to defective POLD1 function is MDPL syndrome (mandibular hypoplasia deafness progeroid features and lipodystrophy) where the genomic instability contributes to the disease phenotype. Through these conditions POLD1 interacts with tumor suppressors and regulatory proteins involved in DNA damage response highlighting its significance in maintaining genomic integrity and preventing disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-POLD1 antibody [EPR15118] (ab186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--western-blot-img104803.jpg "Western blot - Anti-POLD1 antibody [EPR15118] (ab186407)")
Western blot - Anti-POLD1 antibody [EPR15118] (ab186407)
All lanes: Western blot - Anti-POLD1 antibody [EPR15118] (ab186407) at 1/10000 dilution
Lane 1: MOLT4 cell lysate at 20 µg
Lane 2: HeLa cell lysate at 20 µg
Lane 3: Jurkat cell lysate at 20 µg
Lane 4: K562 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 124 kDa
Observed band size: 124 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--immunocytochemistry-immunofluorescence-img17675.jpg "Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)")
Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)
ab186407 staining POLD1, catalytic subunit in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Control: PBS only
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110997.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407)
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling POLD1, catalytic subunit with ab186407 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-POLD1 antibody [EPR15118] (ab186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--flow-cytometry-intracellular-img37734.jpg "Flow Cytometry (Intracellular) - Anti-POLD1 antibody [EPR15118] (ab186407)")
Flow Cytometry (Intracellular) - Anti-POLD1 antibody [EPR15118] (ab186407)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling POLD1, catalytic subunit (red) with purified ab186407 at a dilution of 1/250. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody.
![Western blot - Anti-POLD1 antibody [EPR15118] (ab186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--western-blot-img106459.jpg "Western blot - Anti-POLD1 antibody [EPR15118] (ab186407)")
Western blot - Anti-POLD1 antibody [EPR15118] (ab186407)
All lanes: Western blot - Anti-POLD1 antibody [EPR15118] (ab186407) at 1/1000 dilution
Lane 1: C6 cell lysate at 10 µg
Lane 2: Raw264.7 cell lysate at 10 µg
Lane 3: PC12 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 124 kDa
Observed band size: 124 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123477.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling POLD1, catalytic subunit with ab186407 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--immunocytochemistry-immunofluorescence-img119288.jpg "Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)")
Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling POLD1, catalytic subunit with ab186407 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
![Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--immunocytochemistry-immunofluorescence-img95672.jpg "Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)")
Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)
Immunofluorescent analysis of acetone-fixed K562 cells labeling POLD1, catalytic subunit with ab186407 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123477.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407)")
![Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--immunocytochemistry-immunofluorescence-img119288.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)")
![Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407), expandable thumbnail](https://content.abcam.com/products/images/pold1-antibody-epr15118-ab186407--immunocytochemistry-immunofluorescence-img95672.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)")
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