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Rabbit Recombinant Monoclonal POLD1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (AB240300), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (AB240300), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (AB240300), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (AB240300), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (AB240300), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Expected
Tested
Tested
Tested
Mouse
Expected
Predicted
Predicted
Predicted
Rat
Expected
Predicted
Predicted
Predicted

Expected
Expected

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

-

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Version

3 products for Alternative Product

Target data

Function

As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200, PubMed:24022480). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites (PubMed:19074196, PubMed:24191025).

Alternative names

DNA polymerase delta catalytic subunit, 3'-5' exodeoxyribonuclease, DNA polymerase subunit delta p125, POLD1, POLD

Recommended products

Rabbit Recombinant Monoclonal POLD1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

Alternative names

DNA polymerase delta catalytic subunit, 3'-5' exodeoxyribonuclease, DNA polymerase subunit delta p125, POLD1, POLD

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR15118

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab240300 is the carrier-free version of Anti-POLD1 antibody [EPR15118] ab186407.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

POLD1 also known as DNA polymerase delta is a catalytic subunit of the DNA polymerase delta complex with alternative names such as delta 647 delta 555 delta 488 delta APC delta ALP and ADN-594. Its mass is approximately 124 kDa. POLD1 is expressed in various tissues but shows high expression in proliferating cells. The primary role is to facilitate the high-fidelity DNA replication process by synthesizing the lagging strand during DNA replication and repair. Its enzymatic activity involves adding nucleotides to a primer strand of DNA and executing proofreading functions to ensure replication accuracy.

Biological function summary

POLD1 is integral to the replication machinery where it functions as part of the multi-subunit DNA polymerase delta complex. This complex plays a pivotal role in promoting genome stability by participating in DNA synthesis proofreading and repair processes. The complex provides both polymerase and exonuclease activity ensuring accuracy during DNA replication. Besides POLD1 interacts with PCNA (proliferating cell nuclear antigen) to enhance its enzymatic efficiency during DNA synthesis.

Pathways

POLD1 is involved in the DNA replication and repair pathways. Its interaction with replication protein A (RPA) and PCNA facilitates the smooth completion of Okazaki fragment processing within the DNA replication pathway. Additionally POLD1 plays a role in the base excision repair pathway working alongside other repair proteins such as FEN1 (flap endonuclease 1) and LIG1 (DNA ligase I) to correct DNA lesions. These interactions enforce fidelity and speed during genetic information transfer.

Associated diseases and disorders

Mutations or dysregulation of POLD1 have links to various cancers and genomic instability disorders. Somatic mutations in POLD1 are associated with colorectal cancer where the impaired replication and repair capabilities lead to accumulated mutations. Another disorder related to defective POLD1 function is MDPL syndrome (mandibular hypoplasia deafness progeroid features and lipodystrophy) where the genomic instability contributes to the disease phenotype. Through these conditions POLD1 interacts with tumor suppressors and regulatory proteins involved in DNA damage response highlighting its significance in maintaining genomic integrity and preventing disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)

    Anti-POLD1 antibody [EPR15118] ab186407 staining POLD1, catalytic subunit in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Control: PBS only

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-POLD1 antibody [EPR15118] ab186407).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling POLD1, catalytic subunit with Anti-POLD1 antibody [EPR15118] ab186407 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-POLD1 antibody [EPR15118] ab186407).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling POLD1, catalytic subunit (red) with purified Anti-POLD1 antibody [EPR15118] ab186407 at a dilution of 1/250. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-POLD1 antibody [EPR15118] ab186407).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling POLD1, catalytic subunit with Anti-POLD1 antibody [EPR15118] ab186407 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-POLD1 antibody [EPR15118] ab186407).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling POLD1, catalytic subunit with Anti-POLD1 antibody [EPR15118] ab186407 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-POLD1 antibody [EPR15118] ab186407).

  • Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)

    Immunofluorescent analysis of acetone-fixed K562 cells labeling POLD1, catalytic subunit with Anti-POLD1 antibody [EPR15118] ab186407 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-POLD1 antibody [EPR15118] ab186407).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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