Rabbit Polyclonal POLD4 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human POLD4 aa 50 to C-terminus.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human POLD4 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HCU8
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity (PubMed:16510448, PubMed:19074196, PubMed:20334433). Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system (PubMed:24035200). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb (PubMed:24310611). Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196). Its degradation in response to DNA damage is required for the inhibition of fork progression and cell survival (PubMed:24022480).
Alternative names
POLDS, POLD4, DNA polymerase delta subunit 4, DNA polymerase delta subunit p12
Recommended products
Rabbit Polyclonal POLD4 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human POLD4 aa 50 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human POLD4 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HCU8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
POLD4 also known as DNA polymerase delta subunit 4 functions as a component of the DNA polymerase delta complex assisting in DNA replication and repair mechanisms. This protein has a molecular mass of approximately 12 kDa and is expressed in various tissues including those with high proliferative rates such as the spleen and thymus. As a part of the DNA polymerase delta complex POLD4 contributes to polymerase and exonuclease activities required during cell division.
- Biological function summary
POLD4 plays an integral role in maintaining genomic stability through its function as part of the DNA polymerase delta complex. It is involved in synthesizing the lagging strand during DNA replication and facilitates repair through DNA mismatch repair processes. Its activity ensures accurate DNA synthesis thereby preventing mutations that might otherwise propagate during cell division. Interactions with proliferating cell nuclear antigen (PCNA) and other subunits of the polymerase delta complex POLD1 POLD2 and POLD3 are important for its function.
- Pathways
POLD4 participates in essential DNA replication and repair pathways. It plays a role in the replication fork by ensuring the fidelity of DNA synthesis coordinating with the proteins MSH2 and MLH1 in the mismatch repair pathway. Additionally POLD4's interaction with the replicative DNA helicase complex highlights its involvement in the initiation and elongation phases of DNA replication important for S-phase progression.
- Associated diseases and disorders
POLD4's involvement in DNA replication and repair processes links it to cancer progression. Mutations or dysregulation in POLD4 can lead to genomic instability often observed in various cancers due to defective mismatch repair. The connection between POLD4 and mismatch repair proteins like MSH2 is significant in cancers with microsatellite instability. Additionally POLD4's function is under investigation in relation to neurodegenerative disorders where DNA repair deficits contribute to disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-POLD4 antibody (ab221883)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for POLD4 (green) using ab221883 at 4 μg/ml in ICC/IF.
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