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AB221883

Anti-POLD4 antibody

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(1 Publication)

Rabbit Polyclonal POLD4 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human POLD4 aa 50 to C-terminus.

View Alternative Names

POLDS, POLD4, DNA polymerase delta subunit 4, DNA polymerase delta subunit p12

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-POLD4 antibody (AB221883)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-POLD4 antibody (AB221883)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for POLD4 (green) using ab221883 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human POLD4 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9HCU8

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Complementary Products

Primary Antibodies

AB182564

Anti-POLD3 antibody [EP9480]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-POLD3 antibody [EP9480] (AB182564)

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Proteins & Peptides

AB171583

Recombinant Human POLD4 protein

SDS-PAGE - Recombinant Human POLD4 protein (AB171583)

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Primary Antibodies

AB29

Anti-PCNA antibody [PC10]

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PCNA antibody [PC10] (AB29)

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Primary Antibodies

AB181841

Anti-POLDIP2 antibody [EPR13832]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-POLDIP2 antibody [EPR13832] (AB181841)

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  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

POLD4 also known as DNA polymerase delta subunit 4 functions as a component of the DNA polymerase delta complex assisting in DNA replication and repair mechanisms. This protein has a molecular mass of approximately 12 kDa and is expressed in various tissues including those with high proliferative rates such as the spleen and thymus. As a part of the DNA polymerase delta complex POLD4 contributes to polymerase and exonuclease activities required during cell division.
Biological function summary

POLD4 plays an integral role in maintaining genomic stability through its function as part of the DNA polymerase delta complex. It is involved in synthesizing the lagging strand during DNA replication and facilitates repair through DNA mismatch repair processes. Its activity ensures accurate DNA synthesis thereby preventing mutations that might otherwise propagate during cell division. Interactions with proliferating cell nuclear antigen (PCNA) and other subunits of the polymerase delta complex POLD1 POLD2 and POLD3 are important for its function.

Pathways

POLD4 participates in essential DNA replication and repair pathways. It plays a role in the replication fork by ensuring the fidelity of DNA synthesis coordinating with the proteins MSH2 and MLH1 in the mismatch repair pathway. Additionally POLD4's interaction with the replicative DNA helicase complex highlights its involvement in the initiation and elongation phases of DNA replication important for S-phase progression.

POLD4's involvement in DNA replication and repair processes links it to cancer progression. Mutations or dysregulation in POLD4 can lead to genomic instability often observed in various cancers due to defective mismatch repair. The connection between POLD4 and mismatch repair proteins like MSH2 is significant in cancers with microsatellite instability. Additionally POLD4's function is under investigation in relation to neurodegenerative disorders where DNA repair deficits contribute to disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity (PubMed : 16510448, PubMed : 19074196, PubMed : 20334433). Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system (PubMed : 24035200). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb (PubMed : 24310611). Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed : 19074196). Its degradation in response to DNA damage is required for the inhibition of fork progression and cell survival (PubMed : 24022480).
See full target information POLD4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed36602025

2023

Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Wang,Jing He,Xiaoyu Feng,Shengyou Liao,Yi Zhao,Fuchou Tang,Kehkooi Kee
View all publications
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Product promise

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