AB68663

Anti-POLDIP2 antibody

KO Validated
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(2 Publications)

Rabbit Polyclonal POLDIP2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PDIP38, POLD4, HSPC017, POLDIP2, Polymerase delta-interacting protein 2, 38 kDa DNA polymerase delta interaction protein, p38

2 Images

Lab

Western blot - Anti-POLDIP2 antibody (AB68663)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : POLDIP2 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : HepG2 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab68663 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab68663 was shown to specifically recognize POLDIP2 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when POLDIP2 knockout cells were examined. Wild-type and POLDIP2 knockout samples were subjected to SDS-PAGE. ab68663 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-POLDIP2 antibody (ab68663)

Predicted band size: 42 kDa

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Unknown

Western blot - Anti-POLDIP2 antibody (AB68663)

All lanes:

Western blot - Anti-POLDIP2 antibody (ab68663) at 1 µg/mL

All lanes:

Liver (Human) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 42 kDa

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Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

POLDIP2 also known as Polymerase Delta-Interacting Protein 2 has a molecular mass of approximately 43 kDa. This protein primarily interacts with DNA polymerase delta and plays a role as an essential cofactor involved in DNA replication and repair processes. It is expressed in various tissues with higher levels in the heart and skeletal muscle indicating its importance in these tissues. Through its interaction POLDIP2 regulates the activity and stability of DNA polymerase delta during the cell cycle.

Biological function summary

POLDIP2 is involved in maintaining genomic stability and regulating cellular proliferation. It acts as part of the DNA replication machinery where it assists in the formation and function of the replication fork. POLDIP2 contributes to various cellular functions by interacting with protein complexes which are important for DNA repair mechanisms highlighting its role in preserving genetic information integrity across cell generations.

Pathways

POLDIP2 plays a significant part in the DNA damage response (DDR) pathway. It works with proteins involved in base excision repair and homologous recombination including PCNA and RAD51. The effective cooperation with these proteins ensures that cells proficiently repair DNA errors and maintain cell cycle progression without abnormalities. POLDIP2's role in these pathways highlights its importance in cell survival and proliferation.

POLDIP2 has been connected to cancer and cardiovascular diseases due to its functions in DNA repair and cellular proliferation. Disruption in POLDIP2 expression or function can lead to genomic instability increasing the risk of tumor development. Moreover POLDIP2's involvement in oxidative stress regulation links it to cardiovascular pathologies often associated with endothelial dysfunction. Aberrant interactions with proteins such as Nox4 in endothelial cells can exacerbate these conditions indicating POLDIP2’s potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in DNA damage tolerance by regulating translesion synthesis (TLS) of templates carrying DNA damage lesions such as 8oxoG and abasic sites (PubMed : 24191025). May act by stimulating activity of DNA polymerases involved in TLS, such as PRIMPOL and polymerase delta (POLD1) (PubMed : 24191025, PubMed : 26984527).

See full target information POLDIP2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:2997 PubMed30065243

2018

Systems analysis of intracellular pH vulnerabilities for cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Erez Persi,Miquel Duran-Frigola,Mehdi Damaghi,William R Roush,Patrick Aloy,John L Cleveland,Robert J Gillies,Eytan Ruppin

Experimental dermatology 26:73-81 PubMed27576129

2016

Targeting of NADPH oxidase in vitro and in vivo suppresses fibroblast activation and experimental skin fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Heba Dosoki,Agatha Stegemann,Muna Taha,Hans Schnittler,Thomas A Luger,Katrin Schröder,Jörg H W Distler,Claus Kerkhoff,Markus Böhm

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-POLDIP2 antibody

Western blot - Anti-POLDIP2 antibody (AB68663)

Western blot - Anti-POLDIP2 antibody (AB68663)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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