AB181841

Anti-POLDIP2 antibody [EPR13832]

Name: Anti-POLDIP2 antibody [EPR13832] (ab181841) | Abcam
Brand: Abcam Limited
SKU: AB181841
Price: 355 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(2 Reviews)

(14 Publications)

Rabbit Recombinant Monoclonal POLDIP2 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 14 publications.

View Alternative Names

PDIP38, POLD4, HSPC017, POLDIP2, Polymerase delta-interacting protein 2, 38 kDa DNA polymerase delta interaction protein, p38

4 Images

Flow Cytometry (Intracellular) - Anti-POLDIP2 antibody [EPR13832] (AB181841)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-POLDIP2 antibody [EPR13832] (AB181841)

Intracellular flow cytometric analysis of2% paraformaldehyde-fixed HeLa cells labeling POLDIP2 with ab181841 at 1/350 diltuion (red) compared to aRabbit monoclonal IgG Isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Supplier Data

Immunoprecipitation - Anti-POLDIP2 antibody [EPR13832] (AB181841)

Western blot analysis of HeLa cell lysate immunoprecipitated with ab181841 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-POLDIP2 antibody [EPR13832] (ab181841)

Predicted band size: 42 kDa

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Lab

Western blot - Anti-POLDIP2 antibody [EPR13832] (AB181841)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : POLDIP2 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : HepG2 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab181841 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab181841 was shown to specifically recognize POLDIP2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when POLDIP2 knockout samples were examined. Wild-type and POLDIP2 knockout samples were subjected to SDS-PAGE. ab181841 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1,000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-POLDIP2 antibody [EPR13832] (ab181841)

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-POLDIP2 antibody [EPR13832] (AB181841)

All lanes:

Western blot - Anti-POLDIP2 antibody [EPR13832] (ab181841) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 37 kDa

false

Carrier free

Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13832

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/350", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

POLDIP2 also known as Polymerase Delta-Interacting Protein 2 has a molecular mass of approximately 43 kDa. This protein primarily interacts with DNA polymerase delta and plays a role as an essential cofactor involved in DNA replication and repair processes. It is expressed in various tissues with higher levels in the heart and skeletal muscle indicating its importance in these tissues. Through its interaction POLDIP2 regulates the activity and stability of DNA polymerase delta during the cell cycle.

Biological function summary

POLDIP2 is involved in maintaining genomic stability and regulating cellular proliferation. It acts as part of the DNA replication machinery where it assists in the formation and function of the replication fork. POLDIP2 contributes to various cellular functions by interacting with protein complexes which are important for DNA repair mechanisms highlighting its role in preserving genetic information integrity across cell generations.

Pathways

POLDIP2 plays a significant part in the DNA damage response (DDR) pathway. It works with proteins involved in base excision repair and homologous recombination including PCNA and RAD51. The effective cooperation with these proteins ensures that cells proficiently repair DNA errors and maintain cell cycle progression without abnormalities. POLDIP2's role in these pathways highlights its importance in cell survival and proliferation.

POLDIP2 has been connected to cancer and cardiovascular diseases due to its functions in DNA repair and cellular proliferation. Disruption in POLDIP2 expression or function can lead to genomic instability increasing the risk of tumor development. Moreover POLDIP2's involvement in oxidative stress regulation links it to cardiovascular pathologies often associated with endothelial dysfunction. Aberrant interactions with proteins such as Nox4 in endothelial cells can exacerbate these conditions indicating POLDIP2’s potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in DNA damage tolerance by regulating translesion synthesis (TLS) of templates carrying DNA damage lesions such as 8oxoG and abasic sites (PubMed : 24191025). May act by stimulating activity of DNA polymerases involved in TLS, such as PRIMPOL and polymerase delta (POLD1) (PubMed : 24191025, PubMed : 26984527).

See full target information POLDIP2

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 44:1724-1748 PubMed39934413

2025

Poldip2 promotes mtDNA elimination during Drosophila spermatogenesis to ensure maternal inheritance.

Applications

Unspecified application

Species

Unspecified reactive species

Ziming Wang,Tirawit Meerod,Nuria Cortes-Silva,Ason C-Y Chiang,Ziyan Nie,Ying Di,Peiqiang Mu,Ankit Verma,Adam James Reid,Hansong Ma

Cell communication and signaling : CCS 23:9 PubMed39773189

2025

Polymerase delta-interacting protein 2 mediates brain vascular permeability by regulating ROS-mediated ZO-1 phosphorylation and localization at the interendothelial border.

Applications

Unspecified application

Species

Unspecified reactive species

Keke Wang,Hongyan Qu,Ruinan Hu,Bernard Lassègue,Douglas C Eaton,Chang Song,Jianjun Mu,Kathy K Griendling,Marina S Hernandes

Free radical biology & medicine 199:113-125 PubMed36828293

2023

New insights in the molecular regulation of the NADPH oxidase 2 activity: Negative modulation by Poldip2.

Applications

Unspecified application

Species

Unspecified reactive species

Aicha Bouraoui,Ruy Andrade Louzada,Sana Aimeur,Jehan Waeytens,Frank Wien,Pham My-Chan Dang,Tania Bizouarn,Corinne Dupuy,Laura Baciou

Redox biology 56:102460 PubMed36088760

2022

Capsaicin ameliorates diabetic retinopathy by inhibiting poldip2-induced oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Kou Liu,Xiang Gao,Chengyang Hu,Yanchao Gui,Siyu Gui,Qinyu Ni,Liming Tao,Zhengxuan Jiang

Journal of the American Heart Association 11:e025181 PubMed35535614

2022

Myeloid Poldip2 Contributes to the Development of Pulmonary Inflammation by Regulating Neutrophil Adhesion in a Murine Model of Acute Respiratory Distress Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwei Ou,Elena Dolmatova,Rohan Mandavilli,Hongyan Qu,Georgette Gafford,Taylor White,Alejandra Valdivia,Bernard Lassègue,Marina S Hernandes,Kathy K Griendling

PloS one 16:e0247261 PubMed34928942

2021

Characterization of Poldip2 knockout mice: Avoiding incorrect gene targeting.

Applications

Unspecified application

Species

Unspecified reactive species

Bernard Lassègue,Sandeep Kumar,Rohan Mandavilli,Keke Wang,Michelle Tsai,Dong-Won Kang,Catherine Demos,Marina S Hernandes,Alejandra San Martín,W Robert Taylor,Hanjoong Jo,Kathy K Griendling

Cardiovascular research 118:2506-2518 PubMed34528082

2021

Endothelial Poldip2 regulates sepsis-induced lung injury via Rho pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Elena V Dolmatova,Steven J Forrester,Keke Wang,Ziwei Ou,Holly C Williams,Giji Joseph,Sandeep Kumar,Alejandra Valdivia,Andrew P Kowalczyk,Hongyan Qu,Hanjoong Jo,Bernard Lassègue,Marina S Hernandes,Kathy K Griendling

Scientific reports 11:5533 PubMed33692398

2021

Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction.

Applications

Unspecified application

Species

Unspecified reactive species

Lori N Eidson,Qingzeng Gao,Hongyan Qu,Daniel S Kikuchi,Ana Carolina P Campos,Elizabeth A Faidley,Yu-Yo Sun,Chia-Yi Kuan,Rosana L Pagano,Bernard Lassègue,Malú G Tansey,Kathy K Griendling,Marina S Hernandes

CNS neuroscience & therapeutics 26:1288-1302 PubMed32790044

2020

Poldip2 mediates blood-brain barrier disruption and cerebral edema by inducing AQP4 polarity loss in mouse bacterial meningitis model.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Gao,Weitian Lu,Yue Shu,Zhengyu Yang,Shanquan Sun,Jin Xu,Shengwei Gan,Shujuan Zhu,Guoping Qiu,Fei Zhuo,Shiye Xu,Yiying Wang,Junhong Chen,Xuan Wu,Juan Huang

Journal of neuroinflammation 16:241 PubMed31779628

2019

Poldip2 mediates blood-brain barrier disruption in a model of sepsis-associated encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel S Kikuchi,Ana Carolina P Campos,Hongyan Qu,Steven J Forrester,Rosana L Pagano,Bernard Lassègue,Ruxana T Sadikot,Kathy K Griendling,Marina S Hernandes

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-POLDIP2 antibody [EPR13832]

Flow Cytometry (Intracellular) - Anti-POLDIP2 antibody [EPR13832] (AB181841)

Immunoprecipitation - Anti-POLDIP2 antibody [EPR13832] (AB181841)

Western blot - Anti-POLDIP2 antibody [EPR13832] (AB181841)

Western blot - Anti-POLDIP2 antibody [EPR13832] (AB181841)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise