AB232609

Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal POLDIP2 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PDIP38, POLD4, HSPC017, POLDIP2, Polymerase delta-interacting protein 2, 38 kDa DNA polymerase delta interaction protein, p38

3 Images

Flow Cytometry (Intracellular) - Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (AB232609)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (AB232609)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling POLDIP2 with ab181841 at 1/350 diltuion (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181841).

Supplier Data

Immunoprecipitation - Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (AB232609)

Western blot analysis of HeLa cell lysate immunoprecipitated with ab181841 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181841).

All lanes:

Immunoprecipitation - Anti-POLDIP2 antibody [EPR13832] (<a href='/en-us/products/primary-antibodies/poldip2-antibody-epr13832-ab181841'>ab181841</a>)

Predicted band size: 42 kDa

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Lab

Western blot - Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (AB232609)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : POLDIP2 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : HepG2 whole cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab181841 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab181841 was shown to specifically recognize POLDIP2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when POLDIP2 knockout samples were examined. Wild-type and POLDIP2 knockout samples were subjected to SDS-PAGE. ab181841 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1,000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181841).

All lanes:

Western blot - Anti-POLDIP2 antibody [EPR13832] (<a href='/en-us/products/primary-antibodies/poldip2-antibody-epr13832-ab181841'>ab181841</a>)

Predicted band size: 42 kDa

false

Unconjugated

Anti-POLDIP2 antibody [EPR13832]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13832

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab232609 is the carrier-free version of ab181841.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

POLDIP2 also known as Polymerase Delta-Interacting Protein 2 has a molecular mass of approximately 43 kDa. This protein primarily interacts with DNA polymerase delta and plays a role as an essential cofactor involved in DNA replication and repair processes. It is expressed in various tissues with higher levels in the heart and skeletal muscle indicating its importance in these tissues. Through its interaction POLDIP2 regulates the activity and stability of DNA polymerase delta during the cell cycle.

Biological function summary

POLDIP2 is involved in maintaining genomic stability and regulating cellular proliferation. It acts as part of the DNA replication machinery where it assists in the formation and function of the replication fork. POLDIP2 contributes to various cellular functions by interacting with protein complexes which are important for DNA repair mechanisms highlighting its role in preserving genetic information integrity across cell generations.

Pathways

POLDIP2 plays a significant part in the DNA damage response (DDR) pathway. It works with proteins involved in base excision repair and homologous recombination including PCNA and RAD51. The effective cooperation with these proteins ensures that cells proficiently repair DNA errors and maintain cell cycle progression without abnormalities. POLDIP2's role in these pathways highlights its importance in cell survival and proliferation.

POLDIP2 has been connected to cancer and cardiovascular diseases due to its functions in DNA repair and cellular proliferation. Disruption in POLDIP2 expression or function can lead to genomic instability increasing the risk of tumor development. Moreover POLDIP2's involvement in oxidative stress regulation links it to cardiovascular pathologies often associated with endothelial dysfunction. Aberrant interactions with proteins such as Nox4 in endothelial cells can exacerbate these conditions indicating POLDIP2’s potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in DNA damage tolerance by regulating translesion synthesis (TLS) of templates carrying DNA damage lesions such as 8oxoG and abasic sites (PubMed : 24191025). May act by stimulating activity of DNA polymerases involved in TLS, such as PRIMPOL and polymerase delta (POLD1) (PubMed : 24191025, PubMed : 26984527).

See full target information POLDIP2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 590:344-350 PubMed33505024

2021

Integrated spatial genomics reveals global architecture of single nuclei.

Applications

Unspecified application

Species

Unspecified reactive species

Yodai Takei,Jina Yun,Shiwei Zheng,Noah Ollikainen,Nico Pierson,Jonathan White,Sheel Shah,Julian Thomassie,Shengbao Suo,Chee-Huat Linus Eng,Mitchell Guttman,Guo-Cheng Yuan,Long Cai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (AB232609)

Immunoprecipitation - Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (AB232609)

Western blot - Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (AB232609)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise