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Rabbit Polyclonal POLE antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DNA polymerase epsilon catalytic subunit A.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody (AB110876), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human DNA polymerase epsilon catalytic subunit A. The exact immunogen used to generate this antibody is proprietary information. Database link Q07864

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/100.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Target data

Function

Catalytic component of the DNA polymerase epsilon complex (PubMed:10801849). Participates in chromosomal DNA replication (By similarity). Required during synthesis of the leading DNA strands at the replication fork, binds at/or near replication origins and moves along DNA with the replication fork (By similarity). Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication (By similarity). Involved in DNA synthesis during DNA repair (PubMed:20227374, PubMed:27573199). Along with DNA polymerase POLD1 and DNA polymerase POLK, has a role in excision repair (NER) synthesis following UV irradiation (PubMed:20227374).

Alternative names

Recommended products

Rabbit Polyclonal POLE antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DNA polymerase epsilon catalytic subunit A.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human DNA polymerase epsilon catalytic subunit A. The exact immunogen used to generate this antibody is proprietary information. Database link Q07864
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The POLE protein also known as DNA polymerase epsilon catalytic subunit A functions as an important component in DNA replication and repair mechanisms. POLE possesses a molecular mass of approximately 261 kDa and demonstrates expression primarily in proliferating cells such as those in the bone marrow lymphoid tissues and the gastrointestinal tract. It operates within the eukaryotic DNA polymerase family distinct from other polymerases due to its high fidelity in DNA synthesis which minimizes errors during replication.

Biological function summary

The POLE protein plays an essential role in the high-fidelity synthesis of leading-strand DNA during replication. POLE is part of a larger complex known as the DNA polymerase epsilon holoenzyme collaborating with accessory proteins that enhance its ability to synthesize DNA accurately. Moreover this protein contributes significantly to the proofreading function as it has intrinsic 3' to 5' exonuclease activity allowing the removal of incorrectly paired nucleotides therefore preventing mutations.

Pathways

The POLE protein is fundamentally involved in the DNA replication and repair pathways. These pathways ensure genomic integrity and stability important for normal cell division and preventing genomic instability associated with cancer. POLE interacts with other proteins such as PCNA and RFC (replication factor C) within these pathways facilitating efficient replication fork progression and repair processes. POLE's activity ensures coordination with leading strand synthesis complementing the functions of other polymerases involved in lagging strand DNA synthesis.

Associated diseases and disorders

Mutations in the POLE gene have been linked to colorectal cancer and endometrial cancer. These conditions are often characterized by a hypermutated phenotype arising from impaired DNA proofreading ability due to ineffective exonuclease activity. In this context POLE mutations can assume significance in sporadic cancer cases as well as in hereditary cancer syndromes where it interacts with mismatch repair proteins highlighting its role in maintaining genomic fidelity.

Product promise

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1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody (ab110876), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody (ab110876)

    ab110876, at a 1/50 dilution, staining POLE in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry, in the absence (left panel) or presence (right panel) of the immunizing peptide.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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