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AB317145

Anti-POLE antibody [HL2150]

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Rabbit Recombinant Monoclonal POLE antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human POLE.

View Alternative Names

POLE1, POLE, DNA polymerase epsilon catalytic subunit A, 3'-5' exodeoxyribonuclease, DNA polymerase II subunit A

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody [HL2150] (AB317145)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody [HL2150] (AB317145)

ab317145 detects POLE protein at nucleus by immunohistochemical analysis. Sample : Paraffin-embedded human testis. POLE stained by ab317145 diluted at 1 : 200. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-POLE antibody [HL2150] (AB317145)
  • WB

Supplier Data

Western blot - Anti-POLE antibody [HL2150] (AB317145)

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE and the membrane was blotted with ab317145 diluted at 1 : 2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-POLE antibody [HL2150] (ab317145) at 1/2000 dilution

Lane 1:

K562 whole cell extracts at 30 µg

Lane 2:

THP-1 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-POLE antibody [HL2150] (AB317145)
  • WB

Supplier Data

Western blot - Anti-POLE antibody [HL2150] (AB317145)

Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE and the membrane was blotted with ab317145 diluted at 1 : 900. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-POLE antibody [HL2150] (ab317145) at 1/900 dilution

All lanes:

Mouse testis tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2150

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human POLE.

Q07864

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/900", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The POLE protein also known as DNA polymerase epsilon catalytic subunit A functions as an important component in DNA replication and repair mechanisms. POLE possesses a molecular mass of approximately 261 kDa and demonstrates expression primarily in proliferating cells such as those in the bone marrow lymphoid tissues and the gastrointestinal tract. It operates within the eukaryotic DNA polymerase family distinct from other polymerases due to its high fidelity in DNA synthesis which minimizes errors during replication.
Biological function summary

The POLE protein plays an essential role in the high-fidelity synthesis of leading-strand DNA during replication. POLE is part of a larger complex known as the DNA polymerase epsilon holoenzyme collaborating with accessory proteins that enhance its ability to synthesize DNA accurately. Moreover this protein contributes significantly to the proofreading function as it has intrinsic 3' to 5' exonuclease activity allowing the removal of incorrectly paired nucleotides therefore preventing mutations.

Pathways

The POLE protein is fundamentally involved in the DNA replication and repair pathways. These pathways ensure genomic integrity and stability important for normal cell division and preventing genomic instability associated with cancer. POLE interacts with other proteins such as PCNA and RFC (replication factor C) within these pathways facilitating efficient replication fork progression and repair processes. POLE's activity ensures coordination with leading strand synthesis complementing the functions of other polymerases involved in lagging strand DNA synthesis.

Mutations in the POLE gene have been linked to colorectal cancer and endometrial cancer. These conditions are often characterized by a hypermutated phenotype arising from impaired DNA proofreading ability due to ineffective exonuclease activity. In this context POLE mutations can assume significance in sporadic cancer cases as well as in hereditary cancer syndromes where it interacts with mismatch repair proteins highlighting its role in maintaining genomic fidelity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic component of the DNA polymerase epsilon complex (PubMed : 10801849). Participates in chromosomal DNA replication (By similarity). Required during synthesis of the leading DNA strands at the replication fork, binds at/or near replication origins and moves along DNA with the replication fork (By similarity). Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication (By similarity). Involved in DNA synthesis during DNA repair (PubMed : 20227374, PubMed : 27573199). Along with DNA polymerase POLD1 and DNA polymerase POLK, has a role in excision repair (NER) synthesis following UV irradiation (PubMed : 20227374).
See full target information POLE
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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