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AB201100

Anti-POLG2 antibody

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(1 Publication)

Rabbit Polyclonal POLG2 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human POLG2.

View Alternative Names

MTPOLB, POLG2, DNA polymerase subunit gamma-2, DNA polymerase gamma accessory 55 kDa subunit, Mitochondrial DNA polymerase accessory subunit, MtPolB, PolG-beta, p55

1 Images
Western blot - Anti-POLG2 antibody (AB201100)
  • WB

Supplier Data

Western blot - Anti-POLG2 antibody (AB201100)

All lanes:

Western blot - Anti-POLG2 antibody (ab201100)

Lane 1:

A549 whole cell lysate

Lane 2:

HeLa whole cell lysate

Lane 3:

H9C2 whole cell lysate

Lane 4:

SP20 whole cell lysate

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human POLG2. The exact immunogen used to generate this antibody is proprietary information.

Q9UHN1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab201100 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage buffer
pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

POLG2 also known as the mitochondrial DNA polymerase gamma 2 functions as the accessory subunit of the DNA polymerase gamma complex. This protein has a mass of approximately 55 kDa and primarily localizes in the mitochondria of cells. POLG2 binds tightly to the catalytic subunit POLG enhancing its processivity and fidelity during replication and repair of mitochondrial DNA (mtDNA). Its expression occurs across various tissue types reflecting its essential role in maintaining mitochondrial DNA integrity.
Biological function summary

POLG2 facilitates mtDNA replication by stabilizing the catalytic interaction in the polymerase complex. This complex includes POLG2 and the main polymerase subunit POLG. POLG2 increases the binding affinity of POLG to DNA ensuring efficient and accurate DNA synthesis. The proper functioning of POLG2 in assembling the polymerase complex is necessary for the replication of mtDNA and overall mitochondrial function which directly impacts cell energy production and metabolism.

Pathways

POLG2's activity is integral to the mitochondrial biogenesis and DNA replication pathways. Within these pathways POLG2 works closely with the POLG protein as well as other replication machinery proteins such as TWINKLE helicase. These interactions ensure replication precision and efficiency important for maintaining cellular homeostasis and metabolism. Dysfunction can interrupt the function of these critical pathways leading to reduced mitochondrial output and energy disturbances.

POLG2 mutations have links to mitochondrial diseases such as Progressive External Ophthalmoplegia (PEO) and Alpers-Huttenlocher syndrome. These disorders arise from defects in mtDNA replication often involving POLG mutations that affect the POLG2-POLG complex function. Abnormalities in POLG2 may compound mtDNA mutations precipitating cellular energy deficits and symptomatic manifestations associated with these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory subunit of DNA polymerase gamma solely responsible for replication of mitochondrial DNA (mtDNA). Acts as an allosteric regulator of the holoenzyme activities. Enhances the polymerase activity and the processivity of POLG by increasing its interactions with the DNA template. Suppresses POLG exonucleolytic proofreading especially toward homopolymeric templates bearing mismatched termini. Binds to single-stranded DNA.
See full target information POLG2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:20196 PubMed39215036

2024

Lymphocyte antigen 6 family member E suppresses apoptosis and promotes pancreatic cancer growth and migration via Wnt/β-catenin pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Kenan Cen,Jingyao Zhou,Xuejia Yang,Yangyang Guo,Yanyi Xiao
View all publications
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Product promise

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