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Rabbit Recombinant Monoclonal Poliovirus Receptor/PVR antibody. Suitable for IP, WB and reacts with Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Target data

Function

Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration.(Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.(Microbial infection) Acts as a receptor for Pseudorabies virus.(Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells.

Alternative names

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Rabbit Recombinant Monoclonal Poliovirus Receptor/PVR antibody. Suitable for IP, WB and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR17302

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

The Poliovirus Receptor plays meaningful roles in immune response modulation and cell-cell adhesion. PVR interacts with components of the immune system and forms complexes with other proteins like CD226 and TIGIT. These interactions help regulate immune cell activities especially in the context of natural killer (NK) cells and T-cells. The CD155 protein also links to migration and proliferation processes which are essential for tissue formation and repair.

Activity summary

The Poliovirus Receptor (PVR) also known as CD155 is a cell surface glycoprotein with a molecular mass of approximately 70 kDa. This receptor is expressed on a variety of cell types including epithelial and endothelial cells immune cells and fibroblasts. Another name for PVR is Necl-5 which falls under the nectin-like molecule family. It acts as an adhesion molecule and contributes to cellular signaling and junctional complexes. The expression of PVR is widespread across multiple tissues but it exhibits stronger expression in areas such as the skin and the gastrointestinal tract.

Pathways

PVR is involved in the regulation of immune and signaling pathways. It fits into pathways like NK cell activation and T-cell inhibitory signaling which are important for maintaining immune tolerance and preventing autoimmunity. In these pathways PVR interacts closely with other immunoregulatory proteins including CD226 and TIGIT. The partnership of PVR with these proteins shapes the delicate balance between immune activation and suppression demonstrating a clear role in immune homeostasis.

Associated diseases and disorders

PVR relates to conditions such as cancer and viral infection. Its overexpression or altered signaling has been observed in several cancers where it may contribute to tumor growth and immune evasion. The interaction between PVR and its related protein TIGIT can affect antitumor immune responses complicating cancer progression. Additionally as its name suggests PVR binds to the poliovirus facilitating viral entry and spread during infection. This highlights the importance of PVR not only in pathogenic interactions but also in broader immune response contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunoprecipitation - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304), expandable thumbnail

    Immunoprecipitation - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)

    Poliovirus Receptor/PVR was immunoprecipitated from 1mg of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with ab205304 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205304 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
    Lane 1: U-87 MG whole cell lysate 10μg (Input).
    Lane 2: ab205304 IP in U-87 MG whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205304 in U-87 MG whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

    Developed using the ECL technique.

    Predicted band size: 45 kDa

  • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304), expandable thumbnail

    Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)

    Lanes 1 - 4: Merged signal (red and green). Green - ab205304 observed at 70 kDa (ab205304), 60-80 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab205304 was shown to specifically react with Poliovirus Receptor in wild-type A549 cells as signal was lost in PVR knockout cells. Wild-type and PVR knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab205304 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304) at 1/1000 dilution

    Lane 1: Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: PVR knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 3: U87-MG whole cell lysate at 20 µg

    Lane 4: HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

    Predicted band size: 45 kDa

  • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304), expandable thumbnail

    Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)

    Lanes 1- 4: Merged signal (red and green). Green - ab205304 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

    ab205304 was shown to react with Poliovirus Receptor/PVR in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266102 (knockout cell lysate ab257622) was used. Wild-type HEK-293T and PVR knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab205304 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: PVR knockout HEK-293T cell lysate at 20 µg

    Lane 3: Wild-type A549 cell lysate at 20 µg

    Lane 4: PVR knockout A549 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 45 kDa

    Observed band size: 70 kDa

  • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304), expandable thumbnail

    Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304) at 1/4000 dilution

    Lane 1: HT1080 (Human fibrosarcoma cell line) whole cell lysate at 20 µg

    Lane 2: U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

    Lane 3: HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa

    Observed band size: 70 kDa

    Exposure time: 30s

  • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304), expandable thumbnail

    Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304) at 1/1000 dilution

    All lanes: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa

    Observed band size: 70 kDa

    Exposure time: 3min

  • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304), expandable thumbnail

    Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304) at 1/1000 dilution

    Lane 1: Human fetal heart lysate at 10 µg

    Lane 2: Human fetal kidney lysate at 10 µg

    Lane 3: Human fetal spleen lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa

    Observed band size: 70 kDa

    Exposure time: 3min

  • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304), expandable thumbnail

    Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304) at 1/1000 dilution

    Lane 1: Untreated K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 10 µg

    Lane 2: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate deglycosylation (PNGase F) treated at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa

    Observed band size: 70 kDa

    Exposure time: 3min

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Product protocols

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