AB307687

Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric)

BOND RX™ Validated
20ul selling size
Recombinant
What is this?

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(1 Publication)

Rat Recombinant Monoclonal Poliovirus Receptor/PVR antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD155, PVS, PVR, Poliovirus receptor, Nectin-like protein 5, NECL-5

4 Images

Supplier Data

Immunohistochemistry - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) (AB307687)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Poliovirus Receptor/PVR with ab307687 at 1/100 dilution (10.27 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human bladder cancer. The section was incubated with ab307687 for 30 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Supplier Data

Immunohistochemistry - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) (AB307687)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Poliovirus Receptor/PVR with ab307687 at 1/100 dilution (10.27 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon cancer. The section was incubated with ab307687 for 30 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Supplier Data

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) (AB307687)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : Raji (PMID : 10915595;PMID : 9880562). PVR/CD155 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID : 17893876, 11454801). Exposure time : 26 seconds.

All lanes:

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) (ab307687) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) whole cell lysate 20 μg

Lane 2:

WI-38 (human fetal lung fibroblast) whole cell lysate 20 μg

Lane 3:

HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate 20 μg

Lane 4:

HUVEC (human umbilical vein endothelial cell) whole cell lysate 20 μg

Lane 5:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate 20 μg

Observed band size: 70 kDa

false

Exposure time: 26s

Supplier Data

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) (AB307687)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. PVR/CD155 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID : 17893876, 11454801). Exposure times : Lanes 1-5 : 15 seconds Lanes 6-7 : 81 seconds

All lanes:

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) (ab307687) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate 20 μg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 μg

Observed band size: 70 kDa

false

Exposure time: 180s

Carrier free

Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

EPR22672-151

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

ab307687 is a rat monoclonal chimeric antibody.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Poliovirus Receptor (PVR) also known as CD155 is a cell surface glycoprotein with a molecular mass of approximately 70 kDa. This receptor is expressed on a variety of cell types including epithelial and endothelial cells immune cells and fibroblasts. Another name for PVR is Necl-5 which falls under the nectin-like molecule family. It acts as an adhesion molecule and contributes to cellular signaling and junctional complexes. The expression of PVR is widespread across multiple tissues but it exhibits stronger expression in areas such as the skin and the gastrointestinal tract.

Biological function summary

The Poliovirus Receptor plays meaningful roles in immune response modulation and cell-cell adhesion. PVR interacts with components of the immune system and forms complexes with other proteins like CD226 and TIGIT. These interactions help regulate immune cell activities especially in the context of natural killer (NK) cells and T-cells. The CD155 protein also links to migration and proliferation processes which are essential for tissue formation and repair.

Pathways

PVR is involved in the regulation of immune and signaling pathways. It fits into pathways like NK cell activation and T-cell inhibitory signaling which are important for maintaining immune tolerance and preventing autoimmunity. In these pathways PVR interacts closely with other immunoregulatory proteins including CD226 and TIGIT. The partnership of PVR with these proteins shapes the delicate balance between immune activation and suppression demonstrating a clear role in immune homeostasis.

PVR relates to conditions such as cancer and viral infection. Its overexpression or altered signaling has been observed in several cancers where it may contribute to tumor growth and immune evasion. The interaction between PVR and its related protein TIGIT can affect antitumor immune responses complicating cancer progression. Additionally as its name suggests PVR binds to the poliovirus facilitating viral entry and spread during infection. This highlights the importance of PVR not only in pathogenic interactions but also in broader immune response contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors : CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration.. (Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.. (Microbial infection) Acts as a receptor for Pseudorabies virus.. (Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells.

See full target information PVR

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1430163 PubMed39474422

2024

Single-cell and spatial transcriptome characterize coinhibitory cell-cell communications during histological progression of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Judong Luo,Qianman Gao,Meihua Wang,Hui Liu,Hong Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric)

Immunohistochemistry - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) (AB307687)

Immunohistochemistry - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) (AB307687)

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) (AB307687)

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) (AB307687)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise