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Rat Recombinant Monoclonal Poliovirus Receptor/PVR antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] – Rat IgG1 (Chimeric) – BSA and Azide free (AB307688), expandable thumbnail
  • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] – Rat IgG1 (Chimeric) – BSA and Azide free (AB307688), expandable thumbnail
  • Immunohistochemistry - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 - Chimeric - BSA and Azide free (AB307688), expandable thumbnail
  • Immunohistochemistry - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 - Chimeric - BSA and Azide free (AB307688), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Rat

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-PWB
Human
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration.(Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.(Microbial infection) Acts as a receptor for Pseudorabies virus.(Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells.

Alternative names

PVS, PVS, PVR, Poliovirus receptor, Nectin-like protein 5, NECL-5

Recommended products

Rat Recombinant Monoclonal Poliovirus Receptor/PVR antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR22672-151

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

This rat monoclonal chimeric antibody (ab307688) is a carrier free version of Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) ab307687

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Poliovirus Receptor (PVR) also known as CD155 is a cell surface glycoprotein with a molecular mass of approximately 70 kDa. This receptor is expressed on a variety of cell types including epithelial and endothelial cells immune cells and fibroblasts. Another name for PVR is Necl-5 which falls under the nectin-like molecule family. It acts as an adhesion molecule and contributes to cellular signaling and junctional complexes. The expression of PVR is widespread across multiple tissues but it exhibits stronger expression in areas such as the skin and the gastrointestinal tract.

Biological function summary

The Poliovirus Receptor plays meaningful roles in immune response modulation and cell-cell adhesion. PVR interacts with components of the immune system and forms complexes with other proteins like CD226 and TIGIT. These interactions help regulate immune cell activities especially in the context of natural killer (NK) cells and T-cells. The CD155 protein also links to migration and proliferation processes which are essential for tissue formation and repair.

Pathways

PVR is involved in the regulation of immune and signaling pathways. It fits into pathways like NK cell activation and T-cell inhibitory signaling which are important for maintaining immune tolerance and preventing autoimmunity. In these pathways PVR interacts closely with other immunoregulatory proteins including CD226 and TIGIT. The partnership of PVR with these proteins shapes the delicate balance between immune activation and suppression demonstrating a clear role in immune homeostasis.

Associated diseases and disorders

PVR relates to conditions such as cancer and viral infection. Its overexpression or altered signaling has been observed in several cancers where it may contribute to tumor growth and immune evasion. The interaction between PVR and its related protein TIGIT can affect antitumor immune responses complicating cancer progression. Additionally as its name suggests PVR binds to the poliovirus facilitating viral entry and spread during infection. This highlights the importance of PVR not only in pathogenic interactions but also in broader immune response contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] – Rat IgG1 (Chimeric) – BSA and Azide free (ab307688), expandable thumbnail

    Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] – Rat IgG1 (Chimeric) – BSA and Azide free (ab307688)

    This data was developed using Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) ab307687, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Raji (PMID: 10915595;PMID: 9880562).                                                          

    PVR/CD155 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID:17893876, 11454801).

    Exposure time: 26 seconds.

    All lanes: Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] – Rat IgG1 (Chimeric) (Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) ab307687) at 1/1000 dilution

    Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 20 μg

    Lane 2: WI-38 (human fetal lung fibroblast) whole cell lysate 20 μg

    Lane 3: HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate 20 μg

    Lane 4: HUVEC (human umbilical vein endothelial cell) whole cell lysate 20 μg

    Lane 5: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate 20 μg

    Observed band size: 70 kDa

    Exposure time: 26s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Raji (PMID: 10915595;PMID: 9880562).                                                          

    PVR/CD155 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID:17893876, 11454801).

    Exposure time: 26 seconds.

  • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] – Rat IgG1 (Chimeric) – BSA and Azide free (ab307688), expandable thumbnail

    Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] – Rat IgG1 (Chimeric) – BSA and Azide free (ab307688)

    This data was developed using Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) ab307687, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    PVR/CD155 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID:17893876, 11454801).

    Exposure times:

    Lanes 1-5: 15 seconds

    Lanes 6-7: 81 seconds

    All lanes: Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] – Rat IgG1 (Chimeric) (Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) ab307687) at 1/1000 dilution

    Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate 20 μg

    Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 μg

    Observed band size: 70 kDa

    Exposure time: 180s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    PVR/CD155 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID:17893876, 11454801).

    Exposure times:

    Lanes 1-5: 15 seconds

    Lanes 6-7: 81 seconds

  • Immunohistochemistry - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 - Chimeric - BSA and Azide free (ab307688), expandable thumbnail

    Immunohistochemistry - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 - Chimeric - BSA and Azide free (ab307688)

    This data was developed using Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) ab307687, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Poliovirus Receptor/PVR with Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) ab307687 at 1/100 dilution (10.27 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on human colon cancer.
    The section was incubated with Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) ab307687 for 30 mins at room temperature, followed by anti-rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 - Chimeric - BSA and Azide free (ab307688), expandable thumbnail

    Immunohistochemistry - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 - Chimeric - BSA and Azide free (ab307688)

    This data was developed using Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) ab307687, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Poliovirus Receptor/PVR with Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) ab307687 at 1/100 dilution (10.27 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on human bladder cancer.
    The section was incubated with Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - Rat IgG1 (Chimeric) ab307687 for 30 mins at room temperature, followed by anti-rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

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