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AB246976

Anti-POLR1C antibody

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(1 Publication)

Rabbit Polyclonal POLR1C antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human POLR1C aa 50-200.

View Alternative Names

POLR1E, POLR1C, DNA-directed RNA polymerases I and III subunit RPAC1, DNA-directed RNA polymerase I subunit C, RNA polymerases I and III subunit AC1, AC40, DNA-directed RNA polymerases I and III 40 kDa polypeptide, RPA39, RPC40, RPA40

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-POLR1C antibody (AB246976)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-POLR1C antibody (AB246976)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for POLR1C (green) using ab246976 at 4 μg/ml in ICC/IF.

Western blot - Anti-POLR1C antibody (AB246976)
  • WB

Unknown

Western blot - Anti-POLR1C antibody (AB246976)

All lanes:

Western blot - Anti-POLR1C antibody (ab246976) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

HEK-293T overexpressing POLR1C with C-terminal myc-DDK tag, whole cell lysate.

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human POLR1C aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

O15160

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

POLR1C also known as RNA polymerase I and III subunit C functions as a component of RNA polymerases I and III essential for the synthesis of ribosomal RNA precursors and small RNAs. This protein weighs approximately 34.5 kDa and is found ubiquitously expressed in human tissues reflecting its fundamental role in cellular transcription machinery. POLR1C is a subunit important for these polymerases' proper assembly and function.
Biological function summary

POLR1C participates in the formation of the RNA polymerase I and III complexes which are responsible for transcribing ribosomal RNA and small RNAs respectively. These processes are important for protein synthesis and cell growth. As part of these complexes POLR1C interacts with multiple other subunits to ensure the correct transcription of ribosomal RNA which forms the structural and functional core of ribosomes essential for translating mRNA into proteins.

Pathways

POLR1C is integral to the transcription pathways specifically those conducted by RNA polymerases I and III. These pathways play an important role in the synthesis of ribosomal RNA and small RNAs which are critical for protein biosynthesis. In these processes the protein interacts with other components like RPA proteins as well as RPC5 highlighting its involvement in the transcription cycle critical for cellular function and maintenance.

POLR1C mutations have connections to Treacher Collins syndrome and hypomyelinating leukodystrophy. In Treacher Collins syndrome POLR1C interacts with proteins involved in ribosomal biogenesis disrupting this process and causing craniofacial development issues. Similarly in hypomyelinating leukodystrophy aberrations in ribosomal RNA synthesis contribute to neurological symptoms further implicating POLR1C’s pivotal role in transcription machinery and development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and short non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs, respectively. POLR1C/RPAC1 is part of the polymerase core and may function as a clamp element that moves to open and close the cleft.
See full target information POLR1C
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:751 PubMed40369110

2025

ATF1 and miR-27b-3p drive intervertebral disc degeneration through the PPARG/NF-κB signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Guo,Kun Mu,Jing-Chao Geng,Hai-Yang Xing,Yu Dong,Wen-Dong Liu,Shuan-Chi Wang,Jia-Xiao Shi,Bao-Rui Xing,Jian-Yong Zhao,Xiao-Ming Li
View all publications
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