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AB251223

Anti-POLR1C antibody [EPR16178] - BSA and Azide free

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Rabbit Recombinant Monoclonal POLR1C antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples.

View Alternative Names

POLR1E, POLR1C, DNA-directed RNA polymerases I and III subunit RPAC1, DNA-directed RNA polymerase I subunit C, RNA polymerases I and III subunit AC1, AC40, DNA-directed RNA polymerases I and III 40 kDa polypeptide, RPA39, RPC40, RPA40

2 Images
Western blot - Anti-POLR1C antibody [EPR16178] - BSA and Azide free (AB251223)
  • WB

Supplier Data

Western blot - Anti-POLR1C antibody [EPR16178] - BSA and Azide free (AB251223)

This data was developed using ab196657, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-POLR1C antibody [EPR16178] (<a href='/en-us/products/primary-antibodies/polr1c-antibody-epr16178-ab196657'>ab196657</a>) at 1/10000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 39 kDa

false

Western blot - Anti-POLR1C antibody [EPR16178] - BSA and Azide free (AB251223)
  • WB

Supplier Data

Western blot - Anti-POLR1C antibody [EPR16178] - BSA and Azide free (AB251223)

This data was developed using ab196657, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-POLR1C antibody [EPR16178] (<a href='/en-us/products/primary-antibodies/polr1c-antibody-epr16178-ab196657'>ab196657</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW 264.7cell lysate at 10 µg

Lane 3:

PC12 cell lysate at 10 µg

Lane 4:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16178

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab251223 is the carrier-free version of ab196657.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

POLR1C also known as RNA polymerase I and III subunit C functions as a component of RNA polymerases I and III essential for the synthesis of ribosomal RNA precursors and small RNAs. This protein weighs approximately 34.5 kDa and is found ubiquitously expressed in human tissues reflecting its fundamental role in cellular transcription machinery. POLR1C is a subunit important for these polymerases' proper assembly and function.
Biological function summary

POLR1C participates in the formation of the RNA polymerase I and III complexes which are responsible for transcribing ribosomal RNA and small RNAs respectively. These processes are important for protein synthesis and cell growth. As part of these complexes POLR1C interacts with multiple other subunits to ensure the correct transcription of ribosomal RNA which forms the structural and functional core of ribosomes essential for translating mRNA into proteins.

Pathways

POLR1C is integral to the transcription pathways specifically those conducted by RNA polymerases I and III. These pathways play an important role in the synthesis of ribosomal RNA and small RNAs which are critical for protein biosynthesis. In these processes the protein interacts with other components like RPA proteins as well as RPC5 highlighting its involvement in the transcription cycle critical for cellular function and maintenance.

POLR1C mutations have connections to Treacher Collins syndrome and hypomyelinating leukodystrophy. In Treacher Collins syndrome POLR1C interacts with proteins involved in ribosomal biogenesis disrupting this process and causing craniofacial development issues. Similarly in hypomyelinating leukodystrophy aberrations in ribosomal RNA synthesis contribute to neurological symptoms further implicating POLR1C’s pivotal role in transcription machinery and development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and short non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs, respectively. POLR1C/RPAC1 is part of the polymerase core and may function as a clamp element that moves to open and close the cleft.
See full target information POLR1C
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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