AB104115

Anti-POLR1D antibody

Name: Anti-POLR1D antibody (ab104115) | Abcam
Brand: Abcam Limited
SKU: AB104115

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(2 Publications)

Rabbit Polyclonal RPAC2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

DNA-directed RNA polymerases I and III subunit RPAC2, RNA polymerases I and III subunit AC2, AC19, DNA-directed RNA polymerase I subunit D, RNA polymerase I 16 kDa subunit, RPC16, hRPA19, RPA16, POLR1D

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR1D antibody (AB104115)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR1D antibody (AB104115)

ab104115, at 1/500 dilution, staining POLR1D in HBL435 xenograft by immunohistochemistry.

Unknown

Western blot - Anti-POLR1D antibody (AB104115)

15% SDS-PAGE

All lanes:

Western blot - Anti-POLR1D antibody (ab104115) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 30 µg

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

POLR1D also known as RNA polymerase I and III subunit D is a component of the RNA polymerase I and III complexes. It weighs approximately 16.5 kDa. This protein is important for the transcription of ribosomal RNA (rRNA) and small RNA molecules. It is primarily expressed in cells that have high transcriptional activity such as in tissues like the pancreas thymus and spleen.

Biological function summary

The activity of POLR1D contributes to the formation of ribosomal RNA an essential component of the ribosome which is responsible for protein synthesis. POLR1D forms part of both RNA Polymerase I and RNA Polymerase III complexes which are responsible for transcribing rRNA and other small RNAs respectively. It interacts closely with other subunits within these complexes to facilitate transcription processes.

Pathways

POLR1D plays a role in the rRNA transcription pathway and the RNA polymerase I promoter opening pathway. Its function is essential within these pathways to initiate and sustain the transcription required for ribosome production. POLR1D is related to other subunits of RNA polymerases like POLR1A and POLR1B through these pathways forming an interconnected network essential for nucleolar function and RNA maturation.

Malfunction or mutations within POLR1D can lead to Treacher Collins syndrome which is a disorder affecting craniofacial development. This connection highlights the importance of properly functioning RNA polymerases for developmental processes. Additionally disturbances in POLR1D have been studied in relation to leukemias where disrupted transcription processes can contribute to uncontrolled cell proliferation and cancer. POLR1D's role in these diseases highlights its importance in maintaining normal cellular operation and development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and short non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs, respectively.

See full target information POLR1D

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:837777 PubMed35111765

2022

Cationic Polymer Brush-Modified Carbon Nanotube-Meditated eRNA LINC02569 Silencing Attenuates Nucleus Pulposus Degeneration by Blocking NF-κB Signaling Pathway and Alleviate Cell Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Yulin Huang,Jiaming Yang,Xizhe Liu,Xiaoshuai Wang,Kai Zhu,Zemin Ling,Baozhu Zeng,Ningning Chen,Shaoyu Liu,Fuxin Wei

Developmental dynamics : an official publication o 248:233-246 PubMed30620790

2019

Nuclear FGFR2 regulates musculoskeletal integration within the developing limb.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna E Salva,Ryan R Roberts,Taylor S Stucky,Amy E Merrill

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-POLR1D antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR1D antibody (AB104115)

Western blot - Anti-POLR1D antibody (AB104115)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise