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AB180151

Anti-POLR2E/rpb5 antibody [EPR11750]

3

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal POLR2E/rpb5 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, RPB5 homolog, XAP4, POLR2E

4 Images
Flow Cytometry (Intracellular) - Anti-POLR2E/rpb5 antibody [EPR11750] (AB180151)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-POLR2E/rpb5 antibody [EPR11750] (AB180151)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling POLR2E/rpb5 with ab180151 at 1/10 dilution followed by Goat anti rabbit IgG (FITC) at 1/75 dilution (red), compared to a Rabbit IgG control (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR2E/rpb5 antibody [EPR11750] (AB180151)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR2E/rpb5 antibody [EPR11750] (AB180151)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling POLR2E/rpb5 with ab180151 at 1/100 dilution followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (AB180151)
  • WB

Supplier Data

Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (AB180151)

All lanes:

Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151) at 1/5000 dilution

All lanes:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa

false

Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (AB180151)
  • WB

Supplier Data

Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (AB180151)

All lanes:

Western blot - Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151) at 1/20000 dilution

Lane 1:

A431 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa

false

  • Carrier free

    Anti-POLR2E/rpb5 antibody [EPR11750] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11750

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

POLR2E also known as RPB5 is a subunit of RNA polymerase II which plays a central role in the transcription of DNA into RNA. It weighs approximately 25 kDa. POLR2E is expressed in ubiquitously in human tissues indicating its essential role in basic cellular functions. As a component of the core RNA polymerase II complex it interacts with other polymerase subunits to form the enzyme that synthesizes messenger RNA (mRNA) precursors and many functional non-coding RNAs.
Biological function summary

The function of POLR2E contributes to transcription initiation and elongation processes. It is part of the larger multiprotein complex of RNA polymerase II which consists of twelve different subunits. This complex is responsible for transcribing mRNA from DNA templates in eukaryotic cells. POLR2E enables the assembly of the transcriptional machinery significantly affecting the regulation of gene expression.

Pathways

POLR2E operates in the transcription and mRNA processing pathways. It plays an important role in the synthesis of RNA transcripts that undergo splicing polyadenylation and capping before they become mature mRNAs for protein synthesis. POLR2E interacts with other factors in the transcription complex such as TFIIF and TFIIE which modulate transcriptional efficiency and fidelity.

POLR2E has been linked to transcription-related pathologies such as certain cancers and neurological disorders. Alterations in the expression or function of POLR2E can affect the proper transcription process leading to disrupted cellular functions. It also connects to proteins like BRCA1 in breast cancer where changes in gene expression play a role in tumor development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPABC1 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template.
See full target information POLR2E
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:404 PubMed40854914

2025

Multi-omics analysis reveals RNA polymerase II degradation as a novel mechanism of PF-3758309's anti-tumor activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xinglong Jia,Jingdan Zhang,Lulu Pan,Jingliang He,Mingrui Zhu,Lei Zhao,Xingyu Zhang,Wensi Zhao,Dong Xie,Xiaoyan Shen,Bin Liu,Minjia Tan

eLife 4:e06283 PubMed26067234

2015

MAF1 represses CDKN1A through a Pol III-dependent mechanism.

Applications

ChIP

Species

Human

Yu-Ling Lee,Yuan-Ching Li,Chia-Hsin Su,Chun-Hui Chiao,I-Hsuan Lin,Ming-Ta Hsu
View all publications
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Product promise

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