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AB192407

Anti-POLR2I antibody

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(1 Publication)

Rabbit Polyclonal POLR2I antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human POLR2I.

View Alternative Names

DNA-directed RNA polymerase II subunit RPB9, RNA polymerase II subunit B9, DNA-directed RNA polymerase II subunit I, RNA polymerase II 14.5 kDa subunit, RPB14.5, POLR2I

1 Images
Western blot - Anti-POLR2I antibody (AB192407)
  • WB

Supplier Data

Western blot - Anti-POLR2I antibody (AB192407)

All lanes:

Western blot - Anti-POLR2I antibody (ab192407)

Lane 1:

A549 whole cell lysate

Lane 2:

NIH 3T3 whole cell lysate

Lane 3:

PC12 whole cell lysate

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human POLR2I. The exact immunogen used to generate this antibody is proprietary information.

P36954

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab192407 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage buffer
pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The POLR2I gene encodes a subunit of RNA polymerase II commonly known as RPB9. This subunit weighs about 14 kDa and plays a role in the transcription mechanism. RNA polymerase II carries out the transcription of mRNA from DNA. The POLR2I protein is present in the nucleus of eukaryotic cells. Its zinc finger motifs are essential for the interaction with DNA making its role critical in RNA synthesis.
Biological function summary

POLR2I forms a component of the complex RNA polymerase II. This enzyme complex conducts the synthesis of RNA by reading genetic information coded in DNA. POLR2I contributes to the proper assembly and stability of the polymerase helping in the initiation of transcription. The expression of POLR2I is necessary for effective gene expression regulation influencing cellular growth and differentiation processes.

Pathways

POLR2I participates in the RNA polymerase II transcription pathway which governs the conversion of genetic codes into messenger RNAs. This pathway links closely to the regulation of gene expression levels in response to various developmental and environmental signals. The TFIID complex can regulate RNA polymerase II including subunits like POLR2I. The complex operating alongside POLR2I ensures controlled gene expression necessary for maintaining cellular functions.

Alterations in POLR2I function might relate to transcription disorders that affect gene expression and lead to diseases. Recent studies consider its potential involvement in certain cancers as aberrant transcription and abnormal RNA polymerase II activities often link to oncogenesis. The dysregulation of associated proteins such as p53 also exemplifies how disrupted transcriptional processes can contribute to cancer pathology through POLR2I involvement.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. POLR2I/RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template.
See full target information POLR2I
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 42:966-974 PubMed29767251

2018

Overexpression of PRDM13 inhibits glioma cells via Rho and GTP enzyme activation protein.

Applications

Unspecified application

Species

Unspecified reactive species

Linna Zhang,Huimei Cao,Tao He,Jijuan Yang,Hong Tao,Yin Wang,Qikuan Hu
View all publications
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Product promise

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