Anti-POLR3A antibody (ab96328) is a rabbit polyclonal antibody detecting POLR3A in Western Blot, IP, ChIP. Suitable for Human, Mouse.
- Over 10 publications
- Trusted since 2010
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- Genome biology 23:2462022Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongpeng Jiang,Jie Huang,Kai Tian,Xiao Yi,Haonan Zheng,Yi Zhu,Tiannan Guo,Xiong JiPubMed 36443871
- Nature communications 13:30072022A cancer-associated RNA polymerase III identity drives robust transcription and expression of snaR-A noncoding RNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKevin Van Bortle,David P Marciano,Qing Liu,Tristan Chou,Andrew M Lipchik,Sanjay Gollapudi,Benjamin S Geller,Emma Monte,Rohinton T Kamakaka,Michael P SnyderPubMed 35637192
- Science advances 7:eabh049420215' Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuxi Chen,Wei Xu,Kunpeng Liu,Zheng Jiang,Yang Han,Hongbin Jin,Lin Zhang,Weimin Shen,Shunji Jia,Qianwen Sun,Anming MengPubMed 34797706
- Nature communications 11:64092020Structure of human RNA polymerase III.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEwan Phillip Ramsay et. al.PubMed 33335104
- mBio 11:2020Alteration of the Premature tRNA Landscape by Gammaherpesvirus Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica M Tucker et. al.PubMed 33323507
- Nature communications 11:55402020APOE4 exacerbates synapse loss and neurodegeneration in Alzheimer's disease patient iPSC-derived cerebral organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zhao et. al.PubMed 33139712
- Scientific reports 9:77792019BRAF inhibition sensitizes melanoma cells to α-amanitin via decreased RNA polymerase II assembly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLukas Frischknecht,Christian Britschgi,Patricia Galliker,Yann Christinat,Anton Vichalkovski,Matthias Gstaiger,Werner J Kovacs,Wilhelm KrekPubMed 31123282
- The Journal of biological chemistry 294:7445-74592019Leukodystrophy-associated mutations down-regulate the RNA polymerase III transcript and important regulatory RNA .Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarine Choquet et. al.PubMed 30898877
- eLife 7:2018Changes in mRNA abundance drive shuttling of RNA binding proteins, linking cytoplasmic RNA degradation to transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Gilbertson et. al.PubMed 30281021
- Oncogene 36:6793-68042017Epigenetic regulation of RNA polymerase III transcription in early breast tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ-L Park et. al.PubMed 28846112
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human POLR3A aa 150-500. The exact immunogen used to generate this antibody is proprietary information. Database link O14802
Reactivity data
Select an application| ChIP | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Expected | Predicted |
| Cow | Predicted | Predicted |
ChIP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Mouse | Dilution info - | Notes - |
Target data
Function
Catalytic core component of RNA polymerase III (Pol III), a DNA-dependent RNA polymerase which synthesizes small non-coding RNAs using the four ribonucleoside triphosphates as substrates. Synthesizes 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci (PubMed:19609254, PubMed:19631370, PubMed:20413673, PubMed:33335104, PubMed:33558764, PubMed:33558766, PubMed:34675218, PubMed:35637192, PubMed:9331371). Pol III-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol III is recruited to DNA promoters type I, II or III with the help of general transcription factors and other specific initiation factors. Once the polymerase has escaped from the promoter it enters the elongation phase during which RNA is actively polymerized, based on complementarity with the template DNA strand. Transcription termination involves the release of the RNA transcript and polymerase from the DNA (PubMed:20413673, PubMed:33335104, PubMed:33558764, PubMed:33558766, PubMed:33674783, PubMed:34675218). Forms Pol III active center together with the second largest subunit POLR3B/RPC2. Appends one nucleotide at a time to the 3' end of the nascent RNA, with POLR3A/RPC1 contributing a Mg(2+)-coordinating DxDGD motif, and POLR3B/RPC2 participating in the coordination of a second Mg(2+) ion and providing lysine residues believed to facilitate Watson-Crick base pairing between the incoming nucleotide and template base. Typically, Mg(2+) ions direct a 5' nucleoside triphosphate to form a phosphodiester bond with the 3' hydroxyl of the preceding nucleotide of the nascent RNA, with the elimination of pyrophosphate (PubMed:19609254, PubMed:20413673, PubMed:33335104, PubMed:33558764, PubMed:33674783, PubMed:34675218, PubMed:9331371). Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway.
Alternative names
DNA-directed RNA polymerase III subunit RPC1, RNA polymerase III subunit C1, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase III subunit A, RNA polymerase III 155 kDa subunit, RNA polymerase III subunit C160, RPC155, POLR3A
Recommended products
Anti-POLR3A antibody (ab96328) is a rabbit polyclonal antibody detecting POLR3A in Western Blot, IP, ChIP. Suitable for Human, Mouse.
- Over 10 publications
- Trusted since 2010
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human POLR3A aa 150-500. The exact immunogen used to generate this antibody is proprietary information. Database link O14802
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-POLR3A antibody (ab96328) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), ChIP in Human, Mouse samples.
What is the molecular weight of POLR3A?
Anti-POLR3A (ab96328) specifically detects a band for POLR3A (UniProt: O14802) at a molecular weight of 156kDa.
Trusted by the scientific community
Anti-POLR3A (ab96328) was first used in a scientific publication in 2010 and has been cited over 10 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
POLR3A also known as RNA polymerase III subunit A or RPC1 plays an important role in the transcription machinery of eukaryotic cells. It functions as the largest subunit of RNA polymerase III which is responsible for transcribing small non-coding RNAs such as tRNA and 5S rRNA. POLR3A has a molecular mass of approximately 155 kDa and its expression can be detected across various tissues reflecting its essential role in basic cellular functions.
- Biological function summary
The primary function of POLR3A involves its role as part of the RNA polymerase III complex. This complex synthesizes tRNA and other small RNAs which are important for protein synthesis and various regulatory processes within the cell. POLR3A must work seamlessly with other subunits in the polymerase III complex to ensure efficient RNA production maintaining proper cellular function and growth.
- Pathways
Transcription by POLR3A is vital for synthesis of precursor tRNAs in the transcription pathway. It also plays a part in the immune response pathway known as the cytosolic DNA sensing pathway where POLR3A has been linked to recognition of viral DNA. This involvement indicates a connection with proteins such as RIG-I and MAVS which help mediate responses to pathogenic infections.
- Associated diseases and disorders
Mutations in the POLR3A gene can lead to POLR3-related leukodystrophy a neurological disorder affecting white matter in the brain. Additionally variations in POLR3A have been implicated in certain types of systemic sclerosis an autoimmune disorder. These associations highlight the interplay of POLR3A with disease-related proteins such as POLR3B another subunit in the RNA polymerase III complex which can similarly impact cellular and systemic functions when mutated.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-POLR3A antibody (ab96328)
All lanes: Western blot - Anti-POLR3A antibody (ab96328) at 1/500 dilution
All lanes: HCT116 cell lysate at 30 µg
Predicted band size: 156 kDa
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