Anti-POLR3A antibody

• IP

Supplier Data

Immunoprecipitation - Anti-POLR3A antibody (AB96328)

Immunoprecipitation of POLR3A protein from SK N SH whole cell extracts using 5 µg of ab96328. Western blot analysis was performed using ab96328.

All lanes:

Immunoprecipitation - Anti-POLR3A antibody (ab96328) at 5 µg

Lane 1:

SK N SH whole cell extract

Lane 2:

SK N SH whole cell extract with Control IgG

Lane 3:

SK N SH whole cell extract with ab96328

Secondary

All lanes:

EasyBlot anti Rabbit IgG

Predicted band size: 156 kDa

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• WB

Supplier Data

Western blot - Anti-POLR3A antibody (AB96328)

Samples were separated by 5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-POLR3A antibody (ab96328) at 1/1000 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 156 kDa

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• WB

Supplier Data

Western blot - Anti-POLR3A antibody (AB96328)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-POLR3A antibody (ab96328) at 1/1000 dilution

Lane 1:

Raw264.7 whole cell lysates at 30 µg

Lane 2:

PC 12 whole cell lysate at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 156 kDa

false

• WB

CiteAb

Western blot - Anti-POLR3A antibody (AB96328)

Western Blotting using Anti-POLR3A antibody, ab96328. Publication image from Jia, L. et al., 2020, Nat Commun, 33139712. Legend direct from paper.

Transcriptomics profiling of the iPSC-derived cerebral organoids by WGCNA.a Module-trait relationships between modules and APOE genotype, and between modules and AD status are shown. b, c Top gene ontologies and interaction of genes enriched in the yellow module genes. Purple nodes are hub genes (top 10 highest connectivity). d The mRNA expressions of ERCC4 (APOE4 : p = 0.0576, AD : p = 0.0064, APOE4 x AD : p = 0.0742, Con-E3 vs. AD-E4 : p = 0.0164, Con-E4 vs. AD-E4 : p = 0.0049), DGCR8 (APOE4 : p = 0.1088, AD : p = 0.0469, APOE4 x AD : p = 0.3298, Con-E4 vs. AD-E3 : p = 0.0409), POLR3A (APOE4 : p = 0.3926, AD : p = 0.0004, APOE4 x AD : p = 0.1349, Con-E3 vs. AD-E3 : p = 0.0048, Con-E3 vs. AD-E4 : p = 0.0084, Con-E4 : AD-E3 : p = 0.0221, Con-E4 vs. AD-E4 : p = 0.0484), CLP1 (APOE4 : p = 0.0953, AD : p = 0.0039, APOE4 x AD : p = 0.1831, Con-E3 vs. AD-E4 : p = 0.0157, Con-E4 vs. AD-E4 : p = 0.0066), HSPA4 (APOE4 : p = 0.0501, AD : p = 0.0077, APOE4 x AD : p = 0.0410, Con-E3 vs. AD-E4 : p = 0.0171, Con-E4 vs. AD-E4 : p = 0.0038, AD-E3 vs. AD-E4 : p = 0.0339), and PNO1 (APOE4 : p = 0.8087, AD : p = 0.0022, APOE4 x AD : p = 0.1706, Con-E4 vs. AD-E3 : p = 0.0218, Con-E4 vs. AD-E4 : p = 0.0038) were quantified by RT-qPCR. e Representative images of the immunostaining for G3BP and Tuj1. Scale bar : 50 µm. f–i G3BP, EEA1, and LAMP1 levels were analyzed by western blotting. The levels of G3BP (g; APOE4 : p = 0.6345, AD : p < 0.0001, APOE4 x AD : p = 0.0133, Con-E3 vs. AD-E4 : p = 0.0025, Con-E4 vs. AD-E3 : p = 0.0019, Con-E4 vs. AD-E4 : p < 0.0001), EEA1 (h; APOE4 : p = 0.2792, AD : p = 0.6789, APOE4 x AD : p = 0.0671), and LAMP1 (I; APOE4 : p = 0.2558, AD : p = 0.0954, APOE4 x AD : p = 0.5646) were normalized to β-actin. j–l ERCC4, POLR3A, and HSPA4 were analyzed by western blotting. The levels of ERCC4 (j; APOE4 : p = 0.9762, AD : p = 0.0016, APOE4 x AD : p = 0.3378, Con-E4 vs. AD-E3 : p = 0.0241, Con-E4 vs. AD-E4 : p = 0.0054), POLR3A (k; APOE4 : p = 0.2095, AD : p = 0.0018, APOE4 x AD : p = 0.7764, Con-E4 vs. AD-E3 : p = 0.0048, Con-E4 vs. AD-E4 : p = 0.0149), and HSPA4 (l; APOE4 : p = 0.6526, AD : p = 0.0152, APOE4 x AD : p = 0.2371) were normalized to β-actin. All data are expressed as mean ± SEM (N = 5). ANCOVA for APOE4, AD status, and APOE4 x AD status was performed by including sex, sampling age, and source of iPSCs as co-variables, which was followed by two-sided Tukey–Kramer tests to compare between the groups with two factors (APOE4 and AD status). *p < 0.05, **p < 0.01, ****p < 0.0001.

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