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Rabbit Polyclonal POLR3G antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human POLR3G aa 150 to C-terminus.

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Images

Western blot - Anti-POLR3G antibody (AB200721), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.097% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human POLR3G aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15318

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Species

Mouse

Dilution info

1/500

Notes

-

Species

Rat

Dilution info

1/500

Notes

-

Target data

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed:20413673, PubMed:33558764, PubMed:34675218, PubMed:35637192). Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci (PubMed:20154270, PubMed:20413673, PubMed:35637192). Acts as a long tether that bridges POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer and the mobile stalk of Pol III, coordinating the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state. Pol III exists as two alternative complexes defined by the mutually exclusive incorporation of subunit POLR3G/RPC7alpha or POLR3GL/RPC7beta. POLR3G/RPC7alpha modulates Pol III transcriptome by specifically enhancing the transcription of snaR-A non-coding RNAs. At resting state, occupies the active site of apo Pol III and keeps Pol III in an autoinhibitory mode, preventing non-specific transcription (PubMed:33558764, PubMed:33558766, PubMed:35637192). Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs), induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed:19609254, PubMed:19631370).

Alternative names

Recommended products

Rabbit Polyclonal POLR3G antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human POLR3G aa 150 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human POLR3G aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15318
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

Purity is > 95% (by SDS-PAGE).

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Western blot - Anti-POLR3G antibody (ab200721), expandable thumbnail

    Western blot - Anti-POLR3G antibody (ab200721)

    All lanes: Western blot - Anti-POLR3G antibody (ab200721)

    Lane 1: MCF7 whole cell lysate

    Lane 2: RAW 264.7 whole cell lysate

    Lane 3: H9C2 whole cell lysate

    Predicted band size: 26 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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