Rabbit Polyclonal POLR3G antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human POLR3G aa 150 to C-terminus.
IgG
Rabbit
pH: 7.2
Preservative: 0.097% Sodium azide
Constituents: 99% PBS
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
Mouse | Tested |
Rat | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species Mouse | Dilution info 1/500 | Notes - |
Species Rat | Dilution info 1/500 | Notes - |
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed:20413673, PubMed:33558764, PubMed:34675218, PubMed:35637192). Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci (PubMed:20154270, PubMed:20413673, PubMed:35637192). Acts as a long tether that bridges POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer and the mobile stalk of Pol III, coordinating the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state. Pol III exists as two alternative complexes defined by the mutually exclusive incorporation of subunit POLR3G/RPC7alpha or POLR3GL/RPC7beta. POLR3G/RPC7alpha modulates Pol III transcriptome by specifically enhancing the transcription of snaR-A non-coding RNAs. At resting state, occupies the active site of apo Pol III and keeps Pol III in an autoinhibitory mode, preventing non-specific transcription (PubMed:33558764, PubMed:33558766, PubMed:35637192). Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs), induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed:19609254, PubMed:19631370).
DNA-directed RNA polymerase III subunit RPC7, RNA polymerase III subunit C7, DNA-directed RNA polymerase III subunit G, RNA polymerase III 32 kDa apha subunit, RNA polymerase III 32 kDa subunit, RPC32-alpha, RPC32, POLR3G
Rabbit Polyclonal POLR3G antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human POLR3G aa 150 to C-terminus.
IgG
Rabbit
pH: 7.2
Preservative: 0.097% Sodium azide
Constituents: 99% PBS
Liquid
Polyclonal
Affinity purification Immunogen
Purity is > 95% (by SDS-PAGE).
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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All lanes: Western blot - Anti-POLR3G antibody (ab200721)
Lane 1: MCF7 whole cell lysate
Lane 2: RAW 264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate
Predicted band size: 26 kDa
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