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AB167368

Anti-POLRMT antibody

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Mouse Polyclonal POLRMT antibody. Suitable for WB and reacts with Transfected cell lysate samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human POLRMT.

View Alternative Names

MtRPOL, POLRMT

1 Images
Western blot - Anti-POLRMT antibody (AB167368)
  • WB

Unknown

Western blot - Anti-POLRMT antibody (AB167368)

All lanes:

Western blot - Anti-POLRMT antibody (ab167368) at 1 µg/mL

Lane 1:

POLRMT transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG at 1/2500 dilution

Predicted band size: 139 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human POLRMT.

O00411

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed : 21278163, PubMed : 33602924). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed : 29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed : 29149603). Has DNA primase activity (PubMed : 18685103, PubMed : 33602924). Catalyzes the synthesis of short RNA primers that are necessary for the initiation of lagging-strand DNA synthesis from the origin of light-strand DNA replication (OriL) (PubMed : 18685103, PubMed : 33602924).
See full target information POLRMT
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Product promise

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