JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB228576

Anti-POLRMT antibody

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Polyclonal POLRMT antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POLRMT.

View Alternative Names

MtRPOL, POLRMT

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLRMT antibody (AB228576)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLRMT antibody (AB228576)

Paraffin-embedded human hepatoma tissue stained for POLRMT with ab228576 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-POLRMT antibody (AB228576)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-POLRMT antibody (AB228576)

Methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for POLRMT (green) using ab228576 (1/500 dilution) in ICC/IF.

Counterstain : Hoechst 33342 (blue).

Western blot - Anti-POLRMT antibody (AB228576)
  • WB

Supplier Data

Western blot - Anti-POLRMT antibody (AB228576)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-POLRMT antibody (ab228576) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

HCT116 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 139 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human POLRMT. The exact immunogen used to generate this antibody is proprietary information.

O00411

style
left-column

Alternative Products

Primary Antibodies

AB167368

Anti-POLRMT antibody

Western blot - Anti-POLRMT antibody (AB167368)

  • 0
  • 0
Primary Antibodies

AB93102

Anti-POLRMT antibody

  • 1
  • 1

Complementary Products

Primary Antibodies

AB110242

Anti-NDUFB8 antibody [20E9DH10C12]

Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (AB110242)

  • 5
  • 5
Primary Antibodies

AB224053

Anti-SSBP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (AB224053)

  • 0
  • 0
Primary Antibodies

AB259927

Anti-LRPPRC/GP130 antibody [EPR24052-38]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPPRC/GP130 antibody [EPR24052-38] (AB259927)

  • 0
  • 0
Primary Antibodies

AB300577

Anti-Annexin-11/ANXA11 antibody [EPR26004-63]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-11/ANXA11 antibody [EPR26004-63] (AB300577)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed : 21278163, PubMed : 33602924). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed : 29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed : 29149603). Has DNA primase activity (PubMed : 18685103, PubMed : 33602924). Catalyzes the synthesis of short RNA primers that are necessary for the initiation of lagging-strand DNA synthesis from the origin of light-strand DNA replication (OriL) (PubMed : 18685103, PubMed : 33602924).
See full target information POLRMT
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:290 PubMed40722171

2025

mA-modified CTC-297N7.9 inhibits hepatocellular carcinoma metastasis via epigenetically downregulating CCL2 and CD47.

Applications

Unspecified application

Species

Unspecified reactive species

Huamei Wei,Changhong Pu,Min Zeng,Rongzhou Lu,Yunyu Wei,Yanyan Huang,Zheng Huang,Lizheng Huang,Zuoming Xu,Jianchu Wang,Rihai Ma,Jian Pu

Cell death discovery 8:347 PubMed35922422

2022

The mitochondrial RNA polymerase POLRMT promotes skin squamous cell carcinoma cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yulong Wang,Li Ou,Xirong Li,Tingyu Zheng,Wei-Pei Zhu,Ping Li,Lijun Wu,Tianlan Zhao

Cell death discovery 7:393 PubMed34907167

2021

Identification of mitochondrial RNA polymerase as a potential therapeutic target of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Cai Han,Xiang-Yang Zhang,Peng-Hui Yan,Song-Feng Chen,Fei-Fei Liu,Yun-Rong Zhu,Qing Tian

Cell death & disease 12:751 PubMed34326320

2021

The requirement of mitochondrial RNA polymerase for non-small cell lung cancer cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Zhou,Yong-Hua Sang,Shang Cai,Chun Xu,Min-Hua Shi
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com