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Mouse Monoclonal Polycystin 1/PC1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 12 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (AB74115), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (AB74115), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (AB74115), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBIHC-P
Human
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Predicted
Rat
Not recommended
Not recommended
Predicted

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

1 product for Alternative Version

Target data

Function

Component of a heteromeric calcium-permeable ion channel formed by PKD1 and PKD2 that is activated by interaction between PKD1 and a Wnt family member, such as WNT3A and WNT9B (PubMed:27214281). Both PKD1 and PKD2 are required for channel activity (PubMed:27214281). Involved in renal tubulogenesis (PubMed:12482949). Involved in fluid-flow mechanosensation by the primary cilium in renal epithelium (By similarity). Acts as a regulator of cilium length, together with PKD2 (By similarity). The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling (By similarity). The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling (By similarity). May be an ion-channel regulator. Involved in adhesive protein-protein and protein-carbohydrate interactions. Likely to be involved with polycystin-1-interacting protein 1 in the detection, sequestration and exocytosis of senescent mitochondria (PubMed:37681898).

Alternative names

Polycystin-1, PC1, Autosomal dominant polycystic kidney disease 1 protein, PKD1

Recommended products

Mouse Monoclonal Polycystin 1/PC1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 12 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
7e12
Purification technique
Affinity purification Protein G
Epitope
This antibody was produced to the flank-leucine rich repeat-flank region (24-180aa).
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Polycystin 1 also called PC1 is a large transmembrane protein with a molecular mass of approximately 460 kDa. It primarily expresses in the epithelial cells of renal tubules and also in other tissues like liver and pancreas. PC1 has a role as a mechanosensor and regulates cellular adhesion along with extracellular matrix interactions. It works by forming a complex with another protein known as Polycystin-2 (PC2) and this interaction is important for its function.

Biological function summary

PC1 is involved in processes essential for normal kidney function and development. It is part of a complex that mediates calcium ion entry impacting signaling pathways that regulate cell proliferation differentiation and fluid secretion. This protein's interaction with PC2 through the PC1/PC2 complex is fundamental for its role in these cellular processes. By affecting calcium channels PC1 influences various cell signaling events critical for maintaining cellular homeostasis.

Pathways

PC1 participates in calcium signaling pathways and mechanotransduction. Its role in these pathways links PC1 with the regulation of intracellular calcium levels which is influential in cellular responses to mechanical stimuli. Polycystin-2 is closely associated with PC1 in these pathways as they form a functional polycystin complex that is necessary for these signaling mechanisms.

Associated diseases and disorders

PC1 is significantly associated with autosomal dominant polycystic kidney disease (ADPKD). Mutations in the PC1 gene (PKD1) lead to cyst formation in kidneys and liver indicating its significant role in pathogenesis. Furthermore interactions between PC1 and PC2 are important as abnormalities in either protein can result in ADPKD. Understanding these interactions and the pathways in which PC1 is involved provides insights into potential therapeutic targets for treating related disorders.

Product promise

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3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)

    IHC image of Polycystin 1/PC1 staining in a formalin fixed, paraffin embedded normal human kidney tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74115, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)

    IHC image of Polycystin 1/PC1 staining in Normal Human Bone Marrow formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74115, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (ab74115)

    IHC image of Polycystin 1/PC1 staining in Human Normal Liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74115, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

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