AB235963

Anti-Polycystin 1/PC1 antibody

Name: Anti-Polycystin 1/PC1 antibody (ab235963) | Abcam
Brand: Abcam Limited
SKU: AB235963
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Polycystin 1/PC1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PKD1 aa 600 to C-terminus.

View Alternative Names

Polycystin-1, PC1, Autosomal dominant polycystic kidney disease 1 protein, PKD1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Polycystin 1/PC1 antibody (AB235963)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Polycystin 1/PC1 antibody (AB235963)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Polycystin 1/PC1 (green) using ab235963 at 1/200 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody (AB235963)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody (AB235963)

Paraffin-embedded human colon cancer tissue stained for Polycystin1/PC1 using ab235963 at 1/600 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PKD1 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P98161

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Polycystin 1 also called PC1 is a large transmembrane protein with a molecular mass of approximately 460 kDa. It primarily expresses in the epithelial cells of renal tubules and also in other tissues like liver and pancreas. PC1 has a role as a mechanosensor and regulates cellular adhesion along with extracellular matrix interactions. It works by forming a complex with another protein known as Polycystin-2 (PC2) and this interaction is important for its function.

Biological function summary

PC1 is involved in processes essential for normal kidney function and development. It is part of a complex that mediates calcium ion entry impacting signaling pathways that regulate cell proliferation differentiation and fluid secretion. This protein's interaction with PC2 through the PC1/PC2 complex is fundamental for its role in these cellular processes. By affecting calcium channels PC1 influences various cell signaling events critical for maintaining cellular homeostasis.

Pathways

PC1 participates in calcium signaling pathways and mechanotransduction. Its role in these pathways links PC1 with the regulation of intracellular calcium levels which is influential in cellular responses to mechanical stimuli. Polycystin-2 is closely associated with PC1 in these pathways as they form a functional polycystin complex that is necessary for these signaling mechanisms.

PC1 is significantly associated with autosomal dominant polycystic kidney disease (ADPKD). Mutations in the PC1 gene (PKD1) lead to cyst formation in kidneys and liver indicating its significant role in pathogenesis. Furthermore interactions between PC1 and PC2 are important as abnormalities in either protein can result in ADPKD. Understanding these interactions and the pathways in which PC1 is involved provides insights into potential therapeutic targets for treating related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of a heteromeric calcium-permeable ion channel formed by PKD1 and PKD2 that is activated by interaction between PKD1 and a Wnt family member, such as WNT3A and WNT9B (PubMed : 27214281). Both PKD1 and PKD2 are required for channel activity (PubMed : 27214281). Involved in renal tubulogenesis (PubMed : 12482949). Involved in fluid-flow mechanosensation by the primary cilium in renal epithelium (By similarity). Acts as a regulator of cilium length, together with PKD2 (By similarity). The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling (By similarity). The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling (By similarity). May be an ion-channel regulator. Involved in adhesive protein-protein and protein-carbohydrate interactions. Likely to be involved with polycystin-1-interacting protein 1 in the detection, sequestration and exocytosis of senescent mitochondria (PubMed : 37681898).

See full target information PKD1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed40299470

2025

Polycystins Expression in Astrocytic Gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Martha Assimakopoulou,Konstantina Soufli,Maria Melachrinou

Oncogenesis 12:31 PubMed37244923

2023

Integrated analysis of single-cell and bulk RNA sequencing data reveals an immunostimulatory microenvironment in tumor thrombus of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Ji,Qianyu Shi,Song Mei,Jiuhui Xu,Haijie Liang,Lu Xie,Tingting Ren,Kunkun Sun,Dasen Li,Xiaodong Tang,Peng Zhang,Wei Guo

The Journal of biological chemistry 296:100680 PubMed33872598

2021

Nonredundant roles of DIAPHs in primary ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Oliva Palander,Adam Lam,Richard F Collins,Theo J Moraes,William S Trimble

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Polycystin 1/PC1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Polycystin 1/PC1 antibody (AB235963)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody (AB235963)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise