AB223673

Anti-Polycystin 1/PC1 antibody - C-terminal

Name: Anti-Polycystin 1/PC1 antibody - C-terminal (ab223673) | Abcam
Brand: Abcam Limited
SKU: AB223673

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(1 Publication)

Goat Polyclonal Polycystin 1/PC1 antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PKD1 aa 4250-4300.

View Alternative Names

Polycystin-1, PC1, Autosomal dominant polycystic kidney disease 1 protein, PKD1

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human PKD1 aa 4250-4300. The exact immunogen used to generate this antibody is proprietary information.

P98161

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3-5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab223673 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Polycystin 1 also called PC1 is a large transmembrane protein with a molecular mass of approximately 460 kDa. It primarily expresses in the epithelial cells of renal tubules and also in other tissues like liver and pancreas. PC1 has a role as a mechanosensor and regulates cellular adhesion along with extracellular matrix interactions. It works by forming a complex with another protein known as Polycystin-2 (PC2) and this interaction is important for its function.

Biological function summary

PC1 is involved in processes essential for normal kidney function and development. It is part of a complex that mediates calcium ion entry impacting signaling pathways that regulate cell proliferation differentiation and fluid secretion. This protein's interaction with PC2 through the PC1/PC2 complex is fundamental for its role in these cellular processes. By affecting calcium channels PC1 influences various cell signaling events critical for maintaining cellular homeostasis.

Pathways

PC1 participates in calcium signaling pathways and mechanotransduction. Its role in these pathways links PC1 with the regulation of intracellular calcium levels which is influential in cellular responses to mechanical stimuli. Polycystin-2 is closely associated with PC1 in these pathways as they form a functional polycystin complex that is necessary for these signaling mechanisms.

PC1 is significantly associated with autosomal dominant polycystic kidney disease (ADPKD). Mutations in the PC1 gene (PKD1) lead to cyst formation in kidneys and liver indicating its significant role in pathogenesis. Furthermore interactions between PC1 and PC2 are important as abnormalities in either protein can result in ADPKD. Understanding these interactions and the pathways in which PC1 is involved provides insights into potential therapeutic targets for treating related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of a heteromeric calcium-permeable ion channel formed by PKD1 and PKD2 that is activated by interaction between PKD1 and a Wnt family member, such as WNT3A and WNT9B (PubMed : 27214281). Both PKD1 and PKD2 are required for channel activity (PubMed : 27214281). Involved in renal tubulogenesis (PubMed : 12482949). Involved in fluid-flow mechanosensation by the primary cilium in renal epithelium (By similarity). Acts as a regulator of cilium length, together with PKD2 (By similarity). The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling (By similarity). The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling (By similarity). May be an ion-channel regulator. Involved in adhesive protein-protein and protein-carbohydrate interactions. Likely to be involved with polycystin-1-interacting protein 1 in the detection, sequestration and exocytosis of senescent mitochondria (PubMed : 37681898).

See full target information PKD1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular physiology and biochemistry : internation 51:1982-1995 PubMed30513524

2018

Pituitary Adenylate Cyclase Activating Polypeptide Elicits Neuroprotection Against Acute Ischemic Neuronal Cell Death Associated with NMDA Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Yuji Kaneko,Julian P Tuazon,Xunming Ji,Cesario V Borlongan

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Polycystin 1/PC1 antibody - C-terminal

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise