AB203857

Anti-Polyethylene glycol antibody [EPR21993-154]

RabMAb
Advanced Validation
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Polyethylene glycol antibody. Suitable for FuncS, sELISA, ELISA, WB and reacts with Chemical samples. Cited in 1 publication.

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Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

FuncS

Unknown

Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Method : APS sensor+ Antigen (methoxy PEG800 conjugated to BSA at 0.625 μg/ml) + Blocking (BSA) + Antibody (ab203857 at 2, 1, 0.5, 0.25, 0.125 μg/ml).

Results :

KD (M) = 5.81E-11

kon(1/Ms) = 2.70E+05

kon Error = 6.28E+02

kdis(1/s) = 1.57E-05

kdis Error = 4.49E-07

Full X²= 10.143

Full R²= 0.9993

Testing Biosensor : Aminopropylsilane (APS) Biosensors

Testing machine : Fortebio RED96e

FuncS

Unknown

Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Method : APS sensor+ Antigen (methoxy PEG800 at 25 μg/ml) + Blocking (BSA) + Antibody (ab203857 at 0.5, 0.25, 0.125, 0.0625, 0.03125μg/ml).

Results :

KD (M) = 1.29E-10

kon(1/Ms) = 2.23E+06

kon Error = 1.45E+04

kdis(1/s) = 2.88E-04

kdis Error = 1.74E-06

Full X² = 3.475

Full R² = 0.9865

Testing Biosensor : Aminopropylsilane (APS) Biosensors

Testing machine : Fortebio RED96e

Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

sELISA

Unknown

Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Sandwich ELISA antigen dose-response curve using AffniPure Goat Anti-Mouse IgG (H&L) at 1 μg/ml concentration. The detector antibody was ab203857 anti-Polyethylene glycol at 1 μg/ml. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Antigen concentration range : 0-1000 ng/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS.

Washing Buffer : 1 X TBST.

Substrate : p-nitrophenyl phosphate (PNPP).

Coloration time : 10 min.

sELISA

Unknown

Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Sandwich ELISA antigen dose-response curve using ab203857 at 2 μg/ml concentration. The detector antibody was a anti-Polyethylene glycol antibody (Biotin) (ab53449) at 500 ng/ml. The secondary antibody was a Peroxidase-conjugated Streptavidin at 1/1000 dilution.

Antigen concentration range : 0-1000 ng/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS.

Washing Buffer : 1 X TBST.

Substrate : 3,3',5,5'-Tetramethylbenzidine (TMB).

Coloration time : 2 min.

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

ELISA

Unknown

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Direct ELISA antigen dose-response curve using ab203857 at 0.05 μg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Antigen concentration range : 0-0.05μg/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS.

Washing Buffer : 1 X TBST.

Substrate : p-nitrophenyl phosphate (PNPP).

Coloration time : 15 min.

sELISA

Unknown

Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Antigen concentration range : 0-1000 ng/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS.

Washing Buffer : 1 X TBST.

Substrate : 3,3',5,5'-Tetramethylbenzidine (TMB).

Coloration time : 2 min.

ELISA

Unknown

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Direct ELISA antigen dose-response curve using ab203857 at 0.05 μg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Antigen concentration range : 0-0.05μg/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS.

Washing Buffer : 1 X TBST.

Substrate : p-nitrophenyl phosphate (PNPP).

Coloration time : 15 min.

Lab

Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Exposure time : 92 seconds.

Blocking/Diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857) at 1/1000 dilution

All lanes:

BSA-methoxy PEG 5K, 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 250 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21993-154

Isotype

IgG

Carrier free

Applications

sELISA, ELISA, WB, FuncS

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "FuncS-species-checked": "testedAndGuaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/100", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Polyethylene glycol

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 11:1759-1777 PubMed30913540

2019

Tubastatin A inhibits HDAC and Sirtuin activity rather than being a HDAC6-specific inhibitor in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Jung Choi,Min-Hee Kang,Kwonho Hong,Jin-Hoi Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Polyethylene glycol antibody [EPR21993-154]

Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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