Anti-Polyethylene glycol antibody [EPR21993-154] - BSA and Azide free

• FuncS

Unknown

Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] - BSA and Azide free (AB256481)

Method : APS sensor+ Antigen (methoxy PEG800 at 25 µg/ml) + Blocking (BSA) + Antibody (ab203857 at 0.5, 0.25, 0.125, 0.0625, 0.03125ug/ml).

Results :

KD (M) = 1.29E-10

kon(1/Ms) = 2.23E+06

kon Error = 1.45E+04

kdis(1/s) = 2.88E-04

kdis Error = 1.74E-06

Full X^2 = 3.475

Full R^2 = 0.9865

Testing Biosensor : Aminopropylsilane (APS) Biosensors

Testing machine : Fortebio RED96e

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203857).

• FuncS

Unknown

Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] - BSA and Azide free (AB256481)

Method : APS sensor+ Antigen (methoxy PEG800 conjugated to BSA at 0.625 μg/ml) + Blocking (BSA) + Antibody (ab203857 at 2, 1, 0.5, 0.25, 0.125 µg/ml).

Results :

KD (M) = 5.81E-11

kon(1/Ms) = 2.70E+05

kon Error = 6.28E+02

kdis(1/s) = 1.57E-05

kdis Error = 4.49E-07

Full X²= 10.143

Full R²= 0.9993

Testing Biosensor : Aminopropylsilane (APS) Biosensors

Testing machine : Fortebio RED96e

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203857).

• ELISA

Unknown

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - BSA and Azide free (AB256481)

Direct ELISA antigen dose-response curve using ab203857 at 2 μg/ml concentration. The detector antibody was a anti-Polyethylene glycol antibody (Biotin) (ab53449) at 500 ng/ml. The secondary antibody was a Peroxidase-conjugated Streptavidin at 1/1000 dilution.

Antigen concentration range : 0-1000 ng/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS

Washing Buffer : 1 X TBST

Substrate : 3,3',5,5'-Tetramethylbenzidine (TMB).

Coloration time : 2 min.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203857).

• ELISA

Unknown

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - BSA and Azide free (AB256481)

Direct ELISA antigen dose-response curve using ab203857 at 0.05 μg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Antigen concentration range : 0-0.05μg/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS

Washing Buffer : 1 X TBST

Substrate : p-nitrophenyl phosphate (PNPP).

Coloration time : 15 min.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203857).

• ELISA

Unknown

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - BSA and Azide free (AB256481)

Direct ELISA antigen dose-response curve using ab203857 at 2 μg/ml concentration. The detector antibody was a anti-Polyethylene glycol antibody (Biotin) (ab53449) at 500 ng/ml. The secondary antibody was a Peroxidase-conjugated Streptavidin at 1/1000 dilution.

Antigen concentration range : 0-1000 ng/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS

Washing Buffer : 1 X TBST

Substrate : 3,3',5,5'-Tetramethylbenzidine (TMB).

Coloration time : 2 min.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203857).

• ELISA

Unknown

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - BSA and Azide free (AB256481)

Direct ELISA antigen dose-response curve using AffniPure Goat Anti-Mouse IgG (H&L) at 1 μg/ml concentration. The detector antibody was ab203857 anti-Polyethylene glycol at 1 μg/ml. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Antigen concentration range : 0-1000 ng/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS

Washing Buffer : 1 X TBST

Substrate : p-nitrophenyl phosphate (PNPP).

Coloration time : 10 min.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203857).

• ELISA

Unknown

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - BSA and Azide free (AB256481)

Direct ELISA antigen dose-response curve using ab203857 at 0.05 μg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Antigen concentration range : 0-0.05μg/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS

Washing Buffer : 1 X TBST

Substrate : p-nitrophenyl phosphate (PNPP).

Coloration time : 15 min.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203857).