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AB275332

Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric)

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Mouse Recombinant Monoclonal Polyethylene glycol antibody. Suitable for I-ELISA, WB and reacts with Chemical samples.

View Alternative Names

PEG

2 Images
Indirect ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) (AB275332)
  • I-ELISA

Lab

Indirect ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) (AB275332)

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab275332, 50 ng/ml).

Antigen used at 0-500ng : NH2-PEG 5K-OCH3, NH2-PEG20K-NH2, PEG1000, PEG1000-BSA, mPEG800, mPEG800-BSA.

Secondary antibody used at 1/1000 : Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H&L).

Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) (AB275332)
  • WB

Lab

Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) (AB275332)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) (ab275332) at 1/5000 dilution

All lanes:

BSA-methoxy PEG 5K, 10 ng

Secondary

Lane 1:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Lane 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Observed band size: 250 kDa

false

Exposure time: 30s

  • Carrier free

    Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

EPR21993-154

Isotype

IgG2b

Carrier free

No

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p>Use at 50ng/ml.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody ab203857. By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 59.04% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Polyethylene glycol
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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