Anti-Polyethylene glycol antibody [PEG-B-47] is a rabbit recombinant monoclonal antibody that is used to detect Polyethylene glycol in ELISA, IHC-P, Western blot.
- Recognizes the terminal methoxy group of the PEG molecule
- Does not cross-react with non-specific targets in blood or serum
- Recombinant format for unrivaled batch-batch consistency
- Cited in over 50 publications
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
ELISA | WB | IHC-P | |
---|---|---|---|
Chemical | Tested | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Chemical | Dilution info 0.5-5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chemical | Dilution info 0.015-0.075 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chemical | Dilution info 1/100 | Notes This antibody only works on the tissues when the animals are injected with a PEGylated protein. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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PEG
Anti-Polyethylene glycol antibody [PEG-B-47] is a rabbit recombinant monoclonal antibody that is used to detect Polyethylene glycol in ELISA, IHC-P, Western blot.
- Recognizes the terminal methoxy group of the PEG molecule
- Does not cross-react with non-specific targets in blood or serum
- Recombinant format for unrivaled batch-batch consistency
- Cited in over 50 publications
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
This antibody recognizes the terminal methoxy group of the PEG molecule. This antibody does not cross react with non-specific targets in blood or serum.
ab51257 does not cross react with Tween20.
If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page.
Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. Our anti-mPEG antibody [PEG-B-47] reacts to the methoxy group of Polyethylene glycol (PEG). There is no methoxy group in Tween 20, which should not interfere with the function of this antibody.
Product Specifications
Anti-Polyethylene glycol antibody [PEG-B-47] (ab51257) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ELISA, IHC-P, WB.
Anti-Polyethylene glycol antibody [PEG-B-47] (ab51257) specifically detects Polyethylene glycol (UniProt ID: ; Molecular weight: kDa) and is sold in 100 µg and 1 mg selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Polyethylene glycol antibody [PEG-B-47] (ab51257) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Polyethylene glycol antibody [PEG-B-47] (ab51257) has been cited over 54 times in peer reviewed journals and is trusted by the scientific community.
Related Products
Antibody clone PEG-B-47 is also available pre-conjugated to a variety of labels for your convenience - HRP (HRP Anti-Polyethylene glycol antibody [PEG-B-47] ab202640).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ELISA Graph generated using purified ab51257 at 1μg/mL.
Antigen concentration range:
PEG5000 and PEG20000: 0.0078 - 0.5 μg/mL
ADI-PEG: 0.0156 - 1 μg/mL
PEG-BSA: 0.0039 - 0.25μg/mL
PEG-IFN-α-2b0.0156 - 1 μg/mL
Secondary antibody was an Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG(H+L) at 1/2500.
Blocking/Diluting buffer and concentration 5% NFDM/TBST
Lane 1: Western blot - Anti-Polyethylene glycol antibody [PEG-B-47] (ab51257) at 1/1000 dilution
Lane 2: Western blot - Anti-Polyethylene glycol antibody [PEG-B-47] (ab51257) at 1/5000 dilution
All lanes: Bovine serum albumin (BSA) and PEG 5000 conjugated protein at 0.015 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 280 kDa
Exposure time: 120s
Comparison of sandwich ELISA using RabMAb/RabMAb (ab51257/ab51257) and MAb/RabMAb (Vendor A/ab51257) for capture/detection.
ab51257/ab51257*: Plate coated with 5 ug/mL of #47; 5 ug/mL of #47 used for detection (*Anti-PEG 47 biotin labeled).
Vendor A/ ab51257*: Plates coated with 100 ug/mL of Vendor A Mouse MAb; 5 ug/mL of #47 used for detection (*Anti-PEG 47 biotin labeled).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-Polyethylene glycol antibody [PEG-B-47] (ab51257) at 0.015 µg/mL
All lanes: BSA-PEG at 10 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Method:Protein A sensor + Antigen (PEG 5K-BSA at 0.3μg/ml)+ antibody (ab51257 at 0.0312, 0.0625, 0.125, 0.25, 0.5, 1 μg/m).
Results:
Sample ID: PEG-5K-BSA
KD (M): 2.41E-09
kon(1/Ms): 2.67E+04
kon Error: 6.81E+02
kdis(1/s): 6.43E-05
kdis Error: 2.92E-06
Full X^2: 0.018444
Full R^2: 0.982059
Comparison of 10 ug/well of activated linear (PEG5K) and branched (PEG40K) PEG using 5 ug/ml of ab51257 in Direct ELISA assay.
Accuracy: By detecting the methoxy group of the PEG molecule itself, ab51257 is useful in measuring the pharmacokinetics of PEG-modified molecules in vivo. Data indicate that ab51257 detects various length Y-chain PEG molecules as well as single chain PEG molecules with equal affinity. ab51257 does not cross react with non-specific targets in blood or serum.
ELISA assay using ab51257 to detect different forms of PEG. PEG40000-BSA is a 40 kDa PEG molecule attached to BSA. PEG5000-BSA is a 5 kDa linear PEG molecule attached to BSA.
Comparison of ab51257 and Vendor A mouse MAb in Direct ELISA assy.
Goat anti-rabbit IgG-AP used for anti-PEG-47 detection; goat anti-mouse IgM-AP used for Vendor A MAb detection.
Fig 1a. Direct ELISA using 1 ug/mL of PEG-GCSF.
Fig 1b. Direct ELISA using 1 ug/mL of PEG-IFN.
Comparison to other anti-PEG: In both direct and sandwich ELISA assays, ab51257 shows greater affinity and accuracy than other anti-PEG antibodies when determining the concentration of PEG or PEG-modified proteins. Results were similar whether detecting PEG itself or PEG-modified targets.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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