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Rabbit Recombinant Monoclonal Polyethylene glycol antibody. Carrier free. Suitable for IHC-P, ELISA, sELISA, WB and reacts with Chemical - Human, Chemical samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PELISAsELISAWB
Chemical
Not recommended
Tested
Tested
Tested
Chemical - Human
Expected
Not recommended
Not recommended
Not recommended

Expected
Expected

Species

Chemical - Human

Dilution info

1/250

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Chemical

Dilution info

-

Notes

-

Tested
Tested

Species

Chemical

Dilution info

0.5 µg/mL

Notes

-

Not recommended
Not recommended

Species

Chemical - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Chemical

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Chemical - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Chemical

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Chemical - Human

Dilution info

-

Notes

-

Recommended products

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Rabbit Recombinant Monoclonal Polyethylene glycol antibody. Carrier free. Suitable for IHC-P, ELISA, sELISA, WB and reacts with Chemical - Human, Chemical samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

PEG-B-47b

Purification technique

Affinity purification Protein A

Specificity

ab271853 does not cross react with Tween20.

Dissociation constant

2.41 x 10-9 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

The smallest PEG molecule which our antibody can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab271853 detects both linear and branched PEG based on our in-house ELISA testing.

Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. Our anti-mPEG antibody [PEG-B-47b] reacts to the methoxy group of Polyethylene glycol (PEG). There is no methoxy group in Tween 20, which should not interfere with the function of this antibody.

If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page.

ab271853 is the unconjugated, carrier-free version of ab53449.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853), expandable thumbnail

    ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)

    Direct ELISA antigen dose-response curve using purified ab53449. Antigen concentration of 0-500 ng/mL. A peroxidase-conjugated steptavidin (1/1000) was used.

    Biotin conjugate (ab53449) available for this clone.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).

  • Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853), expandable thumbnail

    Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)

    Sandwich ELISA antigen dose-response curve using purified ab53449.

    Capture antibody - Mouse anti-IFN alpha 2b (4000 ng/ml).

    Antigens - PEG-IFN alpha 2b (0-1000 ng/ml).

    Detection - ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).

  • ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853), expandable thumbnail

    ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)

    ELISA assay using 5 µg/mL Anti-AFP RabMAb as the capture antibody and 0.5 µg/mL Anti-PEG-47-Biotin RabMAb (unpurified ab53449) as the detection antibody. HRP-avidin (1/3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).

  • ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853), expandable thumbnail

    ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)

    Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1/3000) was used for the detection of varying amounts of anti-PEG-47-Biotin (unpurified ab53449).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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