AB271853

Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Polyethylene glycol antibody. Carrier free. Suitable for IHC-P, ELISA, sELISA, WB and reacts with Chemical samples.

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ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853)

ELISA

Unknown

ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853)

ELISA assay using 5 μg/mL Anti-AFP RabMAb as the capture antibody and 0.5 μg/mL Anti-PEG-47-Biotin RabMAb (unpurified ab53449) as the detection antibody. HRP-avidin (1/3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).

Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853)

sELISA

Unknown

Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853)

Sandwich ELISA antigen dose-response curve using purified ab53449.

Capture antibody - Mouse anti-IFN alpha 2b (4000 ng/ml).

Antigens - PEG-IFN alpha 2b (0-1000 ng/ml).

Detection - ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).

ELISA

Unknown

ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853)

Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1/3000) was used for the detection of varying amounts of anti-PEG-47-Biotin (unpurified ab53449).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).

ELISA

Unknown

ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853)

Direct ELISA antigen dose-response curve using purified ab53449. Antigen concentration of 0-500 ng/mL. A peroxidase-conjugated steptavidin (1/1000) was used.

Biotin conjugate (ab53449) available for this clone.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).

Biotin

Biotin Anti-Polyethylene glycol antibody [PEG-B-47b]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

PEG-B-47b

Isotype

IgG

Carrier free

Yes

Applications

sELISA, WB, IHC-P, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab271853 does not cross react with Tween20.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.5 µg/mL", "ELISA-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

The smallest PEG molecule which our antibody can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab271853 detects both linear and branched PEG based on our in-house ELISA testing.

Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. Our anti-mPEG antibody [PEG-B-47b] reacts to the methoxy group of Polyethylene glycol (PEG). There is no methoxy group in Tween 20, which should not interfere with the function of this antibody.

If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page.

ab271853 is the unconjugated, carrier-free version of ab53449.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Polyethylene glycol

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free

ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853)

Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853)

ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853)

ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Product promise