Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Product Insider Program
Be the first to know about our latest product launches - and unlock exclusive offers and discounts.
Rabbit Recombinant Monoclonal Polyethylene glycol antibody. Carrier free. Suitable for IHC-P, ELISA, sELISA, WB and reacts with Chemical - Human, Chemical samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IHC-P | ELISA | sELISA | WB | |
---|---|---|---|---|
Chemical | Not recommended | Tested | Tested | Tested |
Chemical - Human | Expected | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Chemical - Human | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Chemical | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chemical | Dilution info 0.5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chemical - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chemical | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chemical - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chemical | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chemical - Human | Dilution info - | Notes - |
PEG
Rabbit Recombinant Monoclonal Polyethylene glycol antibody. Carrier free. Suitable for IHC-P, ELISA, sELISA, WB and reacts with Chemical - Human, Chemical samples.
PEG
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
PEG-B-47b
Affinity purification Protein A
ab271853 does not cross react with Tween20.
2.41 x 10-9 M
Blue Ice
+4°C
Do Not Freeze
The smallest PEG molecule which our antibody can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab271853 detects both linear and branched PEG based on our in-house ELISA testing.
Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. Our anti-mPEG antibody [PEG-B-47b] reacts to the methoxy group of Polyethylene glycol (PEG). There is no methoxy group in Tween 20, which should not interfere with the function of this antibody.
If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page.
ab271853 is the unconjugated, carrier-free version of ab53449.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Direct ELISA antigen dose-response curve using purified ab53449. Antigen concentration of 0-500 ng/mL. A peroxidase-conjugated steptavidin (1/1000) was used.
Biotin conjugate (ab53449) available for this clone.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).
Sandwich ELISA antigen dose-response curve using purified ab53449.
Capture antibody - Mouse anti-IFN alpha 2b (4000 ng/ml).
Antigens - PEG-IFN alpha 2b (0-1000 ng/ml).
Detection - ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).
ELISA assay using 5 µg/mL Anti-AFP RabMAb as the capture antibody and 0.5 µg/mL Anti-PEG-47-Biotin RabMAb (unpurified ab53449) as the detection antibody. HRP-avidin (1/3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).
Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1/3000) was used for the detection of varying amounts of anti-PEG-47-Biotin (unpurified ab53449).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53449).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com