Rabbit Recombinant Monoclonal Polyethylene glycol antibody. Carrier free. Suitable for IHC-P, ELISA, sELISA, WB and reacts with Chemical samples.
Images
![ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853), expandable thumbnail](https://content.abcam.com/products/images/polyethylene-glycol-antibody-peg-b-47b-bsa-and-azide-free-ab271853--elisa-img116420.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853)")
![Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853), expandable thumbnail](https://content.abcam.com/products/images/polyethylene-glycol-antibody-peg-b-47b-bsa-and-azide-free-ab271853--sandwich-elisa-img121759.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853)")
![ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853), expandable thumbnail](https://content.abcam.com/products/images/polyethylene-glycol-antibody-peg-b-47b-bsa-and-azide-free-ab271853--elisa-img101631.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853)")
![ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853), expandable thumbnail](https://content.abcam.com/products/images/polyethylene-glycol-antibody-peg-b-47b-bsa-and-azide-free-ab271853--elisa-img105522.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (AB271853)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ELISA | sELISA | WB | |
|---|---|---|---|---|
| Chemical | Expected | Tested | Tested | Tested |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 0.5 µg/mL | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Alternative names
PEG
Recommended products
Rabbit Recombinant Monoclonal Polyethylene glycol antibody. Carrier free. Suitable for IHC-P, ELISA, sELISA, WB and reacts with Chemical samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- PEG-B-47b
- Purification technique
- Affinity purification Protein A
- Specificity
ab271853 does not cross react with Tween20.
- Dissociation constant
- 2.41 x 10-9 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
The smallest PEG molecule which our antibody can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab271853 detects both linear and branched PEG based on our in-house ELISA testing.
Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. Our anti-mPEG antibody [PEG-B-47b] reacts to the methoxy group of Polyethylene glycol (PEG). There is no methoxy group in Tween 20, which should not interfere with the function of this antibody.
If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page.
ab271853 is the unconjugated, carrier-free version of Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] ab53449.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853), expandable thumbnail](https://content.abcam.com/products/images/polyethylene-glycol-antibody-peg-b-47b-bsa-and-azide-free-ab271853--elisa-img116420.jpg "ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)")
ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)
Direct ELISA antigen dose-response curve using purified Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] ab53449. Antigen concentration of 0-500 ng/mL. A peroxidase-conjugated steptavidin (1/1000) was used.
Biotin conjugate (Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] ab53449) available for this clone.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] ab53449).
![Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853), expandable thumbnail](https://content.abcam.com/products/images/polyethylene-glycol-antibody-peg-b-47b-bsa-and-azide-free-ab271853--sandwich-elisa-img121759.jpg "Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)")
Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)
Sandwich ELISA antigen dose-response curve using purified Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] ab53449.
Capture antibody - Mouse anti-IFN alpha 2b (4000 ng/ml).
Antigens - PEG-IFN alpha 2b (0-1000 ng/ml).
Detection - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] ab53449).
![ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853), expandable thumbnail](https://content.abcam.com/products/images/polyethylene-glycol-antibody-peg-b-47b-bsa-and-azide-free-ab271853--elisa-img101631.jpg "ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)")
ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)
ELISA assay using 5 µg/mL Anti-AFP RabMAb as the capture antibody and 0.5 µg/mL Anti-PEG-47-Biotin RabMAb (unpurified Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] ab53449) as the detection antibody. HRP-avidin (1/3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] ab53449).
![ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853), expandable thumbnail](https://content.abcam.com/products/images/polyethylene-glycol-antibody-peg-b-47b-bsa-and-azide-free-ab271853--elisa-img105522.jpg "ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)")
ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] - BSA and Azide free (ab271853)
Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1/3000) was used for the detection of varying amounts of anti-PEG-47-Biotin (unpurified Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] ab53449).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] ab53449).
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