Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) is a rabbit monoclonal antibody detecting Polymeric immunoglobulin receptor/PIGR in Western Blot, IP, IHC-P. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020), expandable thumbnail](https://content.abcam.com/products/images/polymeric-immunoglobulin-receptor-pigr-antibody-epr23314-256-ab275020--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132962.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)")
![Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020), expandable thumbnail](https://content.abcam.com/products/images/polymeric-immunoglobulin-receptor-pigr-antibody-epr23314-256-ab275020--immunoprecipitation-img11824.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)")
![Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020), expandable thumbnail](https://content.abcam.com/products/images/polymeric-immunoglobulin-receptor-pigr-antibody-epr23314-256-ab275020--western-blot-img59721.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020), expandable thumbnail](https://content.abcam.com/products/images/polymeric-immunoglobulin-receptor-pigr-antibody-epr23314-256-ab275020--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119503.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)")
![Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020), expandable thumbnail](https://content.abcam.com/products/images/polymeric-immunoglobulin-receptor-pigr-antibody-epr23314-256-ab275020--western-blot-img39277.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)")
Publications
Filter by
- Cellular and molecular life sciences : CMLS 80:572023Relationships of tumor differentiation and immune infiltration in gastric cancers revealed by single-cell RNA-seq analyses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Zhou,Jingwei Yang,Yongqu Lu,Yanpeng Ma,Yan Meng,Qingqing Li,Junpeng Gao,Zhaoyu Jiang,Limei Guo,Wei Wang,Yun Liu,Lu Wen,Miao Kai,Wei Fu,Fuchou TangPubMed 36729271
- Cancer medicine 10:8673-86922021Integrated analysis of patients with KEAP1/NFE2L2/CUL3 mutations in lung adenocarcinomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXing Jin,Yuansheng Zheng,Zhencong Chen,Fei Wang,Guoshu Bi,Ming Li,Jiaqi Liang,Qihai Sui,Yunyi Bian,Zhengyang Hu,Yulei Qiao,Songtao XuPubMed 34617407
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Polymeric immunoglobulin receptor. Mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. Binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment. Secretory component. Through its N-linked glycans ensures anchoring of secretory IgA (sIgA) molecules to mucus lining the epithelial surface to neutralize extracellular pathogens (PubMed:12150896). On its own (free form) may act as a non-specific microbial scavenger to prevent pathogen interaction with epithelial cells (PubMed:16543244).
Alternative names
Polymeric immunoglobulin receptor, PIgR, Poly-Ig receptor, Hepatocellular carcinoma-associated protein TB6, PIGR
Recommended products
Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) is a rabbit monoclonal antibody detecting Polymeric immunoglobulin receptor/PIGR in Western Blot, IP, IHC-P. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR23314-256
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of Polymeric immunoglobulin receptor/PIGR?
Anti-Polymeric immunoglobulin receptor/PIGR [EPR23314-256] (ab275020) specifically detects a band for Polymeric immunoglobulin receptor/PIGR (UniProt: P01833) at a molecular weight of 83kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR23314-256] also available for your convenience:
ab275020, Carrier free - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free ab275030
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The polymeric immunoglobulin receptor often referred to as PIGR or the polymeric Ig receptor is a critical component in the immune system. This protein which has a mass of approximately 83 kDa is expressed primarily on the basolateral surface of epithelial cells. Common expression sites include the respiratory gastrointestinal and genital tracts. PIGR facilitates the transcytosis of polymeric immunoglobulins like IgA and IgM across epithelial cells ensuring that these antibodies reach mucosal sites where they perform functions.
- Biological function summary
The polymeric immunoglobulin receptor plays an essential role in mucosal immunity by transporting IgA and IgM antibodies to mucosal surfaces. The PIGR is a part of a complex that includes the secretory component which protects immunoglobulins from proteolytic degradation. This mechanism is fundamental for safeguarding mucosal surfaces while allowing the immune system to respond swiftly to pathogens.
- Pathways
The interaction of PIGR with polymeric immunoglobulins is an important event in the mucosal immune pathway. This pathway is important in maintaining mucosal homeostasis and pathogen defense. PIGR works closely with immunoglobulin proteins like IgA and IgM enabling their rapid and efficient transport across epithelial barriers. This ensures that the immune system rapidly addresses potential threats at mucosal sites.
- Associated diseases and disorders
Defects in PIGR function can lead to conditions like IgA nephropathy and selective IgA deficiency. These disorders highlight the importance of the polymeric Ig receptor in immune health and suggest its role in maintaining immunoglobulin balance. There is evidence suggesting potential connections between PIGR dysfunction and the proteins involved in immune regulation which helps in understanding how PIGR contributes to disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020), expandable thumbnail](https://content.abcam.com/products/images/polymeric-immunoglobulin-receptor-pigr-antibody-epr23314-256-ab275020--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132962.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on human colon carcinoma (PMID: 19338768). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020), expandable thumbnail](https://content.abcam.com/products/images/polymeric-immunoglobulin-receptor-pigr-antibody-epr23314-256-ab275020--immunoprecipitation-img11824.jpg "Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)")
Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
Polymeric immunoglobulin receptor/PIGR was immunoprecipitated from 0.35 mg Human colon tissue lysate with ab275020 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275020 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human colon tissue lysate 10 ug
Lane 2: ab275020 IP in Human colon tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab275020 in human colon tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
Predicted band size: 83 kDa
Observed band size: 85-100 kDa
![Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020), expandable thumbnail](https://content.abcam.com/products/images/polymeric-immunoglobulin-receptor-pigr-antibody-epr23314-256-ab275020--western-blot-img59721.jpg "Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)")
Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
Low expression cell line: A549 (PMID:21965228).
All lanes: Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) at 1/1000 dilution
Lane 1: Human colon tissue lysate at 20 µg
Lane 2: Human colon cancer tissue lysate at 20 µg
Lane 3: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 85-100 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020), expandable thumbnail](https://content.abcam.com/products/images/polymeric-immunoglobulin-receptor-pigr-antibody-epr23314-256-ab275020--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119503.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on human colonic mucosa (PMID: 19338768). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020), expandable thumbnail](https://content.abcam.com/products/images/polymeric-immunoglobulin-receptor-pigr-antibody-epr23314-256-ab275020--western-blot-img39277.jpg "Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)")
Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
Blocking and dilution buffer:5% NFDM/TBST.
Exposure time: 6 seconds.
All lanes: Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) at 1/1000 dilution
All lanes: His-tagged recombinant human Polymeric immunoglobulin receptor/PIGR protein 10 ng
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020), expandable thumbnail](https://content.abcam.com/products/images/polymeric-immunoglobulin-receptor-pigr-antibody-epr23314-256-ab275020--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85137.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on human kidney (PMID: 31404540). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020), expandable thumbnail](https://content.abcam.com/products/images/polymeric-immunoglobulin-receptor-pigr-antibody-epr23314-256-ab275020--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85137.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)")
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