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AB275020

Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256]

4

(1 Review)

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(5 Publications)

Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) is a rabbit monoclonal antibody detecting Polymeric immunoglobulin receptor/PIGR in Western Blot, IP, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Polymeric immunoglobulin receptor, PIgR, Poly-Ig receptor, Hepatocellular carcinoma-associated protein TB6, PIGR

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human kidney (PMID : 31404540). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human colon carcinoma (PMID : 19338768). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human colonic mucosa (PMID : 19338768). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)
  • IP

Unknown

Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)

Polymeric immunoglobulin receptor/PIGR was immunoprecipitated from 0.35 mg Human colon tissue lysate with ab275020 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275020 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Human colon tissue lysate 10 ug

Lane 2 : ab275020 IP in Human colon tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab275020 in human colon tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

Predicted band size: 83 kDa

Observed band size: 85-100 kDa

false

Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)
  • WB

Supplier Data

Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

Low expression cell line : A549 (PMID : 21965228).

All lanes:

Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human colon cancer tissue lysate at 20 µg

Lane 3:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 83 kDa

Observed band size: 85-100 kDa

false

Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)
  • WB

Supplier Data

Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (AB275020)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 6 seconds.

All lanes:

Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) at 1/1000 dilution

All lanes:

His-tagged recombinant human Polymeric immunoglobulin receptor/PIGR protein 10 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 83 kDa

false

  • Carrier free

    Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23314-256

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (AB109489)

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Primary Antibodies

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Primary Antibodies

AB15580

Anti-Ki67 antibody

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Polymeric immunoglobulin receptor/PIGR?
Anti-Polymeric immunoglobulin receptor/PIGR [EPR23314-256] (ab275020) specifically detects a band for Polymeric immunoglobulin receptor/PIGR (UniProt: P01833) at a molecular weight of 83kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR23314-256] also available for your convenience: ab275020, Carrier free - ab275030

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The polymeric immunoglobulin receptor often referred to as PIGR or the polymeric Ig receptor is a critical component in the immune system. This protein which has a mass of approximately 83 kDa is expressed primarily on the basolateral surface of epithelial cells. Common expression sites include the respiratory gastrointestinal and genital tracts. PIGR facilitates the transcytosis of polymeric immunoglobulins like IgA and IgM across epithelial cells ensuring that these antibodies reach mucosal sites where they perform functions.
Biological function summary

The polymeric immunoglobulin receptor plays an essential role in mucosal immunity by transporting IgA and IgM antibodies to mucosal surfaces. The PIGR is a part of a complex that includes the secretory component which protects immunoglobulins from proteolytic degradation. This mechanism is fundamental for safeguarding mucosal surfaces while allowing the immune system to respond swiftly to pathogens.

Pathways

The interaction of PIGR with polymeric immunoglobulins is an important event in the mucosal immune pathway. This pathway is important in maintaining mucosal homeostasis and pathogen defense. PIGR works closely with immunoglobulin proteins like IgA and IgM enabling their rapid and efficient transport across epithelial barriers. This ensures that the immune system rapidly addresses potential threats at mucosal sites.

Defects in PIGR function can lead to conditions like IgA nephropathy and selective IgA deficiency. These disorders highlight the importance of the polymeric Ig receptor in immune health and suggest its role in maintaining immunoglobulin balance. There is evidence suggesting potential connections between PIGR dysfunction and the proteins involved in immune regulation which helps in understanding how PIGR contributes to disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Polymeric immunoglobulin receptor. Mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. Binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.. Secretory component. Through its N-linked glycans ensures anchoring of secretory IgA (sIgA) molecules to mucus lining the epithelial surface to neutralize extracellular pathogens (PubMed : 12150896). On its own (free form) may act as a non-specific microbial scavenger to prevent pathogen interaction with epithelial cells (PubMed : 16543244).
See full target information PIGR
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:418 PubMed40751216

2025

Human umbilical cord mesenchymal stem cell-derived exosomes enhance follicular regeneration in androgenetic alopecia via activation of Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Aiqing Yu,Yujun Zhang,Saifeng Zhong,Zhizhen Yang,Mingming Xie

Cell reports. Medicine 4:101277 PubMed37944531

2023

Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxue Ren,Mingle Huang,Weixiang Weng,Yubin Xie,Yifan Wu,Shenghua Zhu,Ying Zhang,Dongming Li,Jiaming Lai,Shunli Shen,Jie Lin,Ming Kuang,Xiaoxing Li,Jun Yu,Lixia Xu

Cell cycle (Georgetown, Tex.) 22:986-1004 PubMed36762777

2023

Metformin attenuates multiple myeloma cell proliferation and encourages apoptosis by suppressing METTL3-mediated m6A methylation of THRAP3, RBM25, and USP4.

Applications

Unspecified application

Species

Unspecified reactive species

Cong-Jie Chen,Jie-Yun Huang,Jian-Qing Huang,Jia-Yi Deng,Xiao-Hui Shangguan,Ai-Zhen Chen,Long-Tian Chen,Wei-Hao Wu

Cellular and molecular life sciences : CMLS 80:57 PubMed36729271

2023

Relationships of tumor differentiation and immune infiltration in gastric cancers revealed by single-cell RNA-seq analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhou,Jingwei Yang,Yongqu Lu,Yanpeng Ma,Yan Meng,Qingqing Li,Junpeng Gao,Zhaoyu Jiang,Limei Guo,Wei Wang,Yun Liu,Lu Wen,Miao Kai,Wei Fu,Fuchou Tang

Cancer medicine 10:8673-8692 PubMed34617407

2021

Integrated analysis of patients with KEAP1/NFE2L2/CUL3 mutations in lung adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Jin,Yuansheng Zheng,Zhencong Chen,Fei Wang,Guoshu Bi,Ming Li,Jiaqi Liang,Qihai Sui,Yunyi Bian,Zhengyang Hu,Yulei Qiao,Songtao Xu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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