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Rabbit Recombinant Monoclonal Polymeric immunoglobulin receptor/PIGR antibody. Suitable for IP, WB and reacts with Human samples.

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Images

Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (AB272728), expandable thumbnail
  • Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (AB272728), expandable thumbnail
  • Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (AB272728), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IFIHC-Fr
Human
Tested
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Polymeric immunoglobulin receptor. Mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. Binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment. Secretory component. Through its N-linked glycans ensures anchoring of secretory IgA (sIgA) molecules to mucus lining the epithelial surface to neutralize extracellular pathogens (PubMed:12150896). On its own (free form) may act as a non-specific microbial scavenger to prevent pathogen interaction with epithelial cells (PubMed:16543244).

Alternative names

Polymeric immunoglobulin receptor, PIgR, Poly-Ig receptor, Hepatocellular carcinoma-associated protein TB6, PIGR

Recommended products

Rabbit Recombinant Monoclonal Polymeric immunoglobulin receptor/PIGR antibody. Suitable for IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23314-78
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The polymeric immunoglobulin receptor often referred to as PIGR or the polymeric Ig receptor is a critical component in the immune system. This protein which has a mass of approximately 83 kDa is expressed primarily on the basolateral surface of epithelial cells. Common expression sites include the respiratory gastrointestinal and genital tracts. PIGR facilitates the transcytosis of polymeric immunoglobulins like IgA and IgM across epithelial cells ensuring that these antibodies reach mucosal sites where they perform functions.

Biological function summary

The polymeric immunoglobulin receptor plays an essential role in mucosal immunity by transporting IgA and IgM antibodies to mucosal surfaces. The PIGR is a part of a complex that includes the secretory component which protects immunoglobulins from proteolytic degradation. This mechanism is fundamental for safeguarding mucosal surfaces while allowing the immune system to respond swiftly to pathogens.

Pathways

The interaction of PIGR with polymeric immunoglobulins is an important event in the mucosal immune pathway. This pathway is important in maintaining mucosal homeostasis and pathogen defense. PIGR works closely with immunoglobulin proteins like IgA and IgM enabling their rapid and efficient transport across epithelial barriers. This ensures that the immune system rapidly addresses potential threats at mucosal sites.

Associated diseases and disorders

Defects in PIGR function can lead to conditions like IgA nephropathy and selective IgA deficiency. These disorders highlight the importance of the polymeric Ig receptor in immune health and suggest its role in maintaining immunoglobulin balance. There is evidence suggesting potential connections between PIGR dysfunction and the proteins involved in immune regulation which helps in understanding how PIGR contributes to disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728), expandable thumbnail

    Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728)

    Polymeric immunoglobulin receptor/PIGR was immunoprecipitated from 0.35 mg Human stomach tissue lysate with ab272728 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 272728 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human stomach tissue lysate 10 ug

    Lane 2: ab272728 IP in Human stomach tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab272728 in Human stomach tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds

    All lanes: Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728)

    Predicted band size: 83 kDa

    Observed band size: 85-100 kDa

  • Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728), expandable thumbnail

    Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression cell line: A549 (PMID:21965228).

    Exposure times: Lane 1: 3 minutes; Lane 2: 10 seconds; Lane 3: 3 seconds; Lanes 4-5: 59 seconds.

    All lanes: Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728) at 1/1000 dilution

    Lane 1: Human stomach tissue lysate at 20 µg

    Lane 2: Human colon tissue lysate at 20 µg

    Lane 3: Human colon cancer tissue lysate at 20 µg

    Lane 4: Human lung tissue lysate at 20 µg

    Lane 5: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 83 kDa

    Observed band size: 85-100 kDa

  • Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728), expandable thumbnail

    Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    All lanes: Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728) at 1/1000 dilution

    All lanes: His-tagged Recombinant Human pIgR protein 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 83 kDa

    Observed band size: 100 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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