Anti-Polyoma virus, Large T antigen antibody [CM2B4]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyoma virus, Large T antigen antibody [CM2B4] (AB307450)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Polyoma virus, Large T antigen with ab307450 at 1/2000 (0.2115 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human liver. The section was incubated with ab307450 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyoma virus, Large T antigen antibody [CM2B4] (AB307450)

Immunohistochemical analysis of paraffin-embedded 293T cells labeling Polyoma virus, Large T antigen with ab307450 at 1/2000 (0.2115 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on 293T cells. The section was incubated with ab307450 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyoma virus, Large T antigen antibody [CM2B4] (AB307450)

Immunohistochemical analysis of paraffin-embedded MLK-1 cells labeling Polyoma virus, Large T antigen with ab307450 at 1/2000 (0.2115 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on MLK-1 cells. The section was incubated with ab307450 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• WB

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Western blot - Anti-Polyoma virus, Large T antigen antibody [CM2B4] (AB307450)

Negative control : 293T (PMID : 20444890). The expression profile is consistent with what has been described in the literature (PMID : 20444890). This blot was produced using a 4-12% Bis-Tris gel and MOPS buffer. The gel was run at 200V for 54 minutes and then transferred to a Nitrocellulose membrane at 25V for 10 minutes. The membrane was blocked for an hour using 3% milk and incubated overnight at 4°C with Mouse monoclonal [CM2B4] to Polyoma virus, Large T antigen ( ab307450) and Rabbit anti-ACTN4 (loading control, ab108198) at a 1/1000 and 1/20 000 dilution, respectively. Antibody binding was detected using Goat Anti-Mouse IgG H&L (IRDye® 800CW, green) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD, red) secondary antibodies at a 1/20 000 dilution (1h at room temperature) before imaging. Blocking/Dilution buffer : 3% NFDM/TBST.

All lanes:

Western blot - Anti-Polyoma virus, Large T antigen antibody [CM2B4] (ab307450) at 1/1000 dilution

Lane 1:

MKL-1 whole cell lysate at 20 µg

Lane 2:

293T whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/20000 dilution

Observed band size: 48 kDa

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