AB15085

Anti-Polyoma virus, Medium T antigen antibody [PyMT]

Name: Anti-Polyoma virus, Medium T antigen antibody [PyMT] (ab15085) | Abcam
Brand: Abcam Limited
SKU: AB15085
Rating: 4 (1 reviews)

(1 Review)

(21 Publications)

Rat Monoclonal MT antibody. Suitable for IP, WB, ELISA, ICC/IF and reacts with Polyomavirus, Synthetic peptide - Polyomavirus samples. Cited in 21 publications.

View Alternative Names

Middle T antigen, MT, MT-AG

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

PyMT

Isotype

IgG2b

Carrier free

Reacts with

Polyomavirus

Applications

ICC/IF, IP, WB, ELISA

applications

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Polyomavirus": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide - Polyomavirus": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Binds medium T antigen only, allows isolation of viral T antigens.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Polyoma virus Medium T antigen also called Middle T antigen belongs to the early proteins expressed by Polyomaviruses. Medium T antigen has a molecular mass of about 57 kilodaltons (kDa). This protein is located in the cytoplasm and is anchored to the cellular membrane. Its expression occurs predominantly during the early phase of viral infection. The Medium T antigen has an important role in transforming host cells primarily through its interactions with cellular signaling components.

Biological function summary

The Middle T antigen facilitates oncogenic transformation by associating with host cell proteins that regulate various signaling pathways. This protein engages in forming a complex with cellular kinases such as Src family kinases. By acting as a scaffold protein it effectively recruits and activates these kinases which contributes to unregulated cell growth. Through these interactions Medium T antigen alters normal cell signaling dynamics and promotes cellular transformation an important step in viral proliferation.

Pathways

The Middle T antigen integrates into signaling networks that control cell growth and division. It prominently interfaces with the PI3K/Akt pathway which is essential for cell proliferation and survival. In this context the Medium T antigen interacts closely with Protein Tyrosine Kinase-driven pathways including the Src kinase pathway influencing downstream targets. These interactions facilitate abnormal activation of signaling projects linked to cancer development.

The Middle T antigen's role links closely to cancer formation specifically in relation to its ability to induce tumorigenesis. Its interactions with proteins like the Src family kinases can lead to uncontrolled cellular proliferation and transformation phenomena observed in cancerous conditions. The aberrant engagement of Medium T antigen with PI3K further emphasizes its connection to cancer as dysregulated PI3K/Akt signaling is common in various malignancies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Plays a role in transformation by modulating the activities of cellular proteins involved in control of cell proliferation and by acting as a functional homolog of an activated tyrosine kinase-associated growth-factor receptor. Recruits upon association with host Ppp2/PP2A the Src tyrosine kinase components Src, Yes and Fyn, thereby activating their kinase activity. Activation of Shc1, Pclg1 and p85 mediate signal transduction pathways leading to cell cycle progression and cell division. MT also plays a role in regulation of early and late gene expression and in viral DNA replication.

See full target information MT_POVMA

style

left-column

Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 42:474-486.e12 PubMed38402610

2024

Chronic stress increases metastasis via neutrophil-mediated changes to the microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Yan He,Yuan Gao,David Ng,Evdokia Michalopoulou,Shanu George,Jose M Adrover,Lijuan Sun,Jean Albrengues,Juliane Daßler-Plenker,Xiao Han,Ledong Wan,Xiaoli Sky Wu,Longling S Shui,Yu-Han Huang,Bodu Liu,Chang Su,David L Spector,Christopher R Vakoc,Linda Van Aelst,Mikala Egeblad

Science advances 8:eabo1673 PubMed36399580

2022

Diabetic hyperglycemia promotes primary tumor progression through glycation-induced tumor extracellular matrix stiffening.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Wang,Lauren A Hapach,Lauren Griggs,Kyra Smart,Yusheng Wu,Paul V Taufalele,Matthew M Rowe,Katherine M Young,Madison E Bates,Andrew C Johnson,Nicholas J Ferrell,Ambra Pozzi,Cynthia A Reinhart-King

Cancer cell 39:1361-1374.e9 PubMed34478639

2021

Activating a collaborative innate-adaptive immune response to control metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Sun,Tim Kees,Ana Santos Almeida,Bodu Liu,Xue-Yan He,David Ng,Xiao Han,David L Spector,Iain A McNeish,Phyllis Gimotty,Sylvia Adams,Mikala Egeblad

Cell 183:395-410.e19 PubMed33007268

2020

Regulation of Collective Metastasis by Nanolumenal Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Emma D Wrenn,Ami Yamamoto,Breanna M Moore,Yin Huang,Margaux McBirney,Aaron J Thomas,Erin Greenwood,Yuri F Rabena,Habib Rahbar,Savannah C Partridge,Kevin J Cheung

The Journal of experimental medicine 217: PubMed32667673

2020

Cancer cell CCR2 orchestrates suppression of the adaptive immune response.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam R Fein,Xue-Yan He,Ana S Almeida,Emilis Bružas,Arnaud Pommier,Ran Yan,Anaïs Eberhardt,Douglas T Fearon,Linda Van Aelst,John Erby Wilkinson,Camila O Dos Santos,Mikala Egeblad

Developmental cell 54:593-607.e5 PubMed32668208

2020

Genetic Fate Mapping of Transient Cell Fate Reveals N-Cadherin Activity and Function in Tumor Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Zan Lv,Shaohua Zhang,Zhuo Wang,Lingjuan He,Muxue Tang,Wenjuan Pu,Huan Zhao,Zhenqian Zhang,Qihui Shi,Dongqing Cai,Mingfu Wu,Guohong Hu,Kathy O Lui,Jing Feng,M Angela Nieto,Bin Zhou

Oncoimmunology 9:1685300 PubMed32002300

2020

A pipeline for identification and validation of tumor-specific antigens in a mouse model of metastatic breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Christa I DeVette,Harika Gundlapalli,Shu-Chin Alicia Lai,Curtis P McMurtrey,Ashley R Hoover,Hem R Gurung,Wei R Chen,Alana L Welm,William H Hildebrand

Nature genetics 50:1705-1715 PubMed30349115

2018

Long noncoding RNA MALAT1 suppresses breast cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jongchan Kim,Hai-Long Piao,Beom-Jun Kim,Fan Yao,Zhenbo Han,Yumeng Wang,Zhenna Xiao,Ashley N Siverly,Sarah E Lawhon,Baochau N Ton,Hyemin Lee,Zhicheng Zhou,Boyi Gan,Shinichi Nakagawa,Matthew J Ellis,Han Liang,Mien-Chie Hung,M James You,Yutong Sun,Li Ma

Clinical cancer research : an official journal of 23:6165-6177 PubMed28855348

2017

Timing of PD-1 Blockade Is Critical to Effective Combination Immunotherapy with Anti-OX40.

Applications

Unspecified application

Species

Unspecified reactive species

David J Messenheimer,Shawn M Jensen,Michael E Afentoulis,Keith W Wegmann,Zipei Feng,David J Friedman,Michael J Gough,Walter J Urba,Bernard A Fox

Cancer research 76:6424-6435 PubMed27569213

2016

Ablation of miR-10b Suppresses Oncogene-Induced Mammary Tumorigenesis and Metastasis and Reactivates Tumor-Suppressive Pathways.

Applications

ICC/IF

Species

Unspecified reactive species

Jongchan Kim,Ashley N Siverly,Dahu Chen,Min Wang,Yuan Yuan,Yumeng Wang,Hyemin Lee,Jinsong Zhang,William J Muller,Han Liang,Boyi Gan,Xianbin Yang,Yutong Sun,M James You,Li Ma

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Polyoma virus, Medium T antigen antibody [PyMT]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

Product promise