Rabbit Polyclonal UBC antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human UBC aa 1-50.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human UBC aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P0CG48
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
Associated Products
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Target data
Function
Ubiquitin. Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. During ubiquitination, the acceptor ubiquitin is positioned in the active site via direct interaction with the E2 ubiquitin-conjugating enzymes such as UBE2R2 (PubMed:38326650).
Alternative names
Polyubiquitin-C, UBC
Recommended products
Rabbit Polyclonal UBC antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human UBC aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human UBC aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P0CG48
- Purification technique
- Precipitation Ammonium Sulphate
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 6 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Polyubiquitin-C often referred to simply as UBC plays an important role in the process of polyubiquitination. This protein with a mass of approximately 26.0 kDa is found in various tissues throughout the body and is expressed ubiquitously. It participates actively in tagging substrates for degradation by the proteasome effectively maintaining protein homeostasis. Polyubiquitin-C consists of a repeating sequence of ubiquitin which serves as a signal for the proteasome to recognize and degrade the tagged protein.
- Biological function summary
Polyubiquitin-C functions in diverse cellular processes beyond protein degradation. It is part of broader complexes involved in DNA repair cell cycle regulation and response to oxidative stress. By attaching ubiquitin molecules to target proteins polyubiquitin-C facilitates their recognition and processing by specific cellular machinery. These roles ensure the cell can degrade misfolded or damaged proteins preventing their accumulation and potential cytotoxic effects.
- Pathways
Polyubiquitin-C plays a critical role in the ubiquitin-proteasome system and NF-kB signaling. It interacts with E3 ubiquitin ligases like Mdm2 which adds ubiquitin molecules to p53 marking it for degradation. This pathway has implications for cell cycle regulation and apoptosis. Polyubiquitin-C also regulates NF-kB signaling by modifying components like IκBα controlling its stability and therefore NF-kB activation. These relationships place polyubiquitin-C at the center of numerous signaling networks.
- Associated diseases and disorders
Dysfunctions associated with polyubiquitin-C can lead to cancer and neurodegenerative diseases. Overactivity in its pathway is often linked to cancerous transformations due to improper degradation of tumor suppressors such as p53. Similarly in neurodegenerative diseases like Alzheimer's polyubiquitin-C's role in degrading misfolded proteins becomes central. Failure to manage protein aggregates due to pathway impairments can result in cytotoxicity. The involvement with proteins like tau further reveals polyubiquitin-C's influence in these contexts emphasizing its importance in cellular health and disease management.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyubiquitin-C antibody (ab104651)
ab104651 at 10μg/mL, staining Polyubiquitin-C in formalin-fixed paraffin-embedded Human kidney tissue.
Western blot - Anti-Polyubiquitin-C antibody (ab104651)
All lanes: Western blot - Anti-Polyubiquitin-C antibody (ab104651) at 1/100 dilution
All lanes: HeLa cell lysate
Predicted band size: 77 kDa
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