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AB32893

Anti-POMC antibody

3

(5 Reviews)

|

(35 Publications)

Goat Polyclonal POMC antibody. Suitable for ELISA, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 35 publications. Immunogen corresponding to Synthetic Peptide within Human POMC aa 250 to C-terminus.

View Alternative Names

Pro-opiomelanocortin, POMC, Corticotropin-lipotropin

2 Images
Flow Cytometry (Intracellular) - Anti-POMC antibody (AB32893)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-POMC antibody (AB32893)

Flow cytometry analysis of paraformaldehyde fixed A431 (human epidermoid carcinoma cell line) cells (blue line) labeling POMC with ab32893 at 10 μg/mL. Cells permeabilized with 0.5% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 1 μg/mL.

IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody (AB32893)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody (AB32893)

Immunohistochemical analysis of Human pituitary gland labeling POMC with ab32893. Antigen retrieval by steaming with Tris/EDTA buffer pH 9. HRP-staining.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ELISA, Flow Cyt (Intra), WB

applications

Immunogen

Synthetic Peptide within Human POMC aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P01189

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/128000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.3-2 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. This product appears to react with the full length precursor protein only.</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Corticotropin. Stimulates the adrenal glands to release cortisol.. Melanocyte-stimulating hormone alpha. Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes.. Melanocyte-stimulating hormone beta. Increases the pigmentation of skin by increasing melanin production in melanocytes.. Beta-endorphin. Endogenous orexigenic opiate.. Met-enkephalin. Endogenous opiate.
See full target information POMC

Publications (35)

Recent publications for all applications. Explore the full list and refine your search

Food science & nutrition 13:e70601 PubMed40708775

2025

Analysis of the Aerial Parts of Champ. and Mechanistic Study of Its Active Ingredient Formononetin in Improving Metabolic Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Niu,Ruhai Jian,Lishen Zeng,Ziyue Huang,Jinyan Cai

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 19:36 PubMed39042202

2024

Semaglutide Attenuates Anxious and Depressive-Like Behaviors and Reverses the Cognitive Impairment in a Type 2 Diabetes Mellitus Mouse Model Via the Microbiota-Gut-Brain Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Igor Henrique Rodrigues de Paiva,Rodrigo Soares da Silva,Ingrid Prata Mendonça,José Roberto Botelho de Souza,Christina Alves Peixoto

Frontiers in pharmacology 14:1233468 PubMed37521477

2023

The pharmacological effects and safety of the raw and prepared folium of Maxim. on improving kidney-yang deficiency syndrome and sexual dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Wang,Juntao Li,Xinyu Zheng,Jian Xu,Zhe Wang,Senjie Li,Qiang Yang,Yue Wu,Dong-Hua Yang,Shen Yao,Xiangwei Zheng

iScience 26:106670 PubMed37168577

2023

Casein kinase 1α is required to maintain murine hypothalamic pro-opiomelanocortin expression.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyang Lu,Jinglin Zhang,Bingjie Wang,Qiao Gao,Kezhe Ma,Shaona Pei,Juxue Li,Sheng Cui

Neuroendocrinology 113:844-858 PubMed36948162

2023

miRNA-383 and miRNA-384 Suppress Proopiomelanocortin Gene Expression in the Hypothalamus: Effects of Early Life Ethanol Exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Omkaram Gangisetty,Shaista Chaudhary,Prashant Tarale,Miguel A Cabrera,Dipak K Sarkar

Endocrinology 163: PubMed35678423

2022

Aberrant Nuclear Translocation of E2F1 and Its Association in Cushing's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Takako Araki,Justin Wang,Ryan Lawrence,Yasuhiko Kawakami

Brain research 1786:147901 PubMed35367433

2022

Sex differences and the lack of effects of chemogenetic manipulation of pro-opiomelanocortin (POMC) neurons on alcohol consumption in male and female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jonna M Leyrer-Jackson,Lauren E Hood,M Foster Olive

Frontiers in endocrinology 12:727915 PubMed34526970

2021

Upregulation of in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control.

Applications

Unspecified application

Species

Unspecified reactive species

Dongxiao Zhang,Satoshi Yamaguchi,Xinhao Zhang,Boxuan Yang,Naoko Kurooka,Ryosuke Sugawara,Haya Hamed H Albuayjan,Atsuko Nakatsuka,Jun Eguchi,Takeshi Y Hiyama,Atsunori Kamiya,Jun Wada

Journal of cellular and molecular medicine 25:2404-2417 PubMed33491272

2021

Targeting the TR4 nuclear receptor with antagonist bexarotene can suppress the proopiomelanocortin signalling in AtT-20 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liqun Xia,Danyang Shen,Youyun Zhang,Jieyang Lu,Mingchao Wang,Huan Wang,Yuanlei Chen,Dingwei Xue,Dajiang Xie,Gonghui Li

Nature communications 11:5772 PubMed33188191

2020

Primary cilia mediate early life programming of adiposity through lysosomal regulation in the developing mouse hypothalamus.

Applications

Unspecified application

Species

Unspecified reactive species

Chan Hee Lee,Do Kyeong Song,Chae Beom Park,Jeewon Choi,Gil Myoung Kang,Sung Hoon Shin,Ijoo Kwon,Soyoung Park,Seongjun Kim,Ji Ye Kim,Hong Dugu,Jae Woo Park,Jong Han Choi,Se Hee Min,Jong-Woo Sohn,Min-Seon Kim
View all publications

Product promise

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