Name: Anti-POMC antibody [EPR22534-165]
SKU: ab254257
Rating: 4 (1 reviews)

Rabbit Recombinant Monoclonal POMC antibody. Suitable for WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

Images

Western blot - Anti-POMC antibody [EPR22534-165] (AB254257), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	IHC-Fr	IHC-P
Human	Not recommended	Tested	Expected	Expected	Tested
Mouse	Not recommended	Tested	Tested	Tested	Tested
Rat	Not recommended	Tested	Expected	Tested	Tested

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -
Species Rat	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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3 products for Alternative Product

Target data

See full target information POMC

Function

Corticotropin. Stimulates the adrenal glands to release cortisol. Melanocyte-stimulating hormone alpha. Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes. Melanocyte-stimulating hormone beta. Increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin. Endogenous orexigenic opiate. Met-enkephalin. Endogenous opiate.

Alternative names

Pro-opiomelanocortin, POMC, Corticotropin-lipotropin

Recommended products

Rabbit Recombinant Monoclonal POMC antibody. Suitable for WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR22534-165
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-POMC antibody [EPR22534-165] (ab254257)
Exposure time:
Lanes 1-2: 3 seconds;
Lanes 3-4: 10 seconds;
Lanes 5-6: 32 seconds
Blocking buffer and concentration: 5% NFDM/TBST
Negative control: liver (lanes 2, 4 and 6, PMID: 10395956)
POMC is a glycoprotein and can be processed into 16kDa N-POMC. The molecular weight observed is consistent with what has been described in the literature (PMID 1814247, PMID 26880796, PMID 20639461).
Lanes 1 - 4: Western blot - Anti-POMC antibody [EPR22534-165] (ab254257) at 1/5000 dilution
Lanes 5 - 6: Western blot - Anti-POMC antibody [EPR22534-165] (ab254257) at 1/1000 dilution
Lane 1: Mouse pituitary lysate at 20 µg
Lane 2: Mouse liver lysate at 20 µg
Lane 3: Human pituitary lysate at 20 µg
Lane 4: Human liver lysate at 20 µg
Lane 5: Rat pituitary lysate at 20 µg
Lane 6: Rat liver lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 16 kDa, 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] (ab254257)
Immunohistochemical analysis of paraffin-embedded human pituitary tissue labeling POMC with ab254257 at 1/4000 dilution at room temperature for 30 mins, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human pituitary is observed (PMID: 21622576). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was performed. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] (ab254257)
Negative control:No staining on mouse spleen (PMID: 10395956)
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling POMC with ab254257 at 1/4000 dilution at room temperature for 30 mins, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was performed. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] (ab254257)
Immunohistochemical analysis of 0.2% Triton X-100 permeabilized, 4% PFA fixed mouse pituitary tissue labeling POMC with ab254257 at 1/500 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. Positive staining on mouse pituitary (PMID: 18601691).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution
Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] (ab254257)
Negative control: No stain on liver (PMID: 14578285)
Immunohistochemical analysis of 0.2% Triton X-100 permeabilized, 4% PFA fixed mouse liver tissue labeling POMC with ab254257 at 1/100 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. No staining on mouse liver.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] (ab254257)
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue labeling POMC with ab254257 at 1/4000 dilution at room temperature for 30 mins, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat pituitary is observed (PMID: 21622576). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was performed. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] (ab254257)
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling POMC with ab254257 at 1/4000 dilution at room temperature for 30 mins, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse pituitary is observed (PMID: 21622576). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was performed. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] (ab254257)
Negative control: No stain on liver (PMID: 14578285)
Immunohistochemical analysis of 0.2% Triton X-100 permeabilized, 4% PFA fixed rat liver tissue labeling POMC with ab254257 at 1/100 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. No staining on rat liver.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution
Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] (ab254257)
Immunohistochemical analysis of 0.2% Triton X-100 permeabilized, 4% PFA fixed rat pituitary tissue labeling POMC with ab254257 at 1/500 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. Positive staining on rat pituitary (PMID: 18601691).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution
Immunocytochemistry/ Immunofluorescence - Anti-POMC antibody [EPR22534-165] (ab254257)
Immunocytochemical analysis of 100% methanol fixed, 0.1% TritonX-100 permeabilised AtT-20 cells labeling POMC with ab254257 at 1/100 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as a secondary antibody at 1/1000 dilution. Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution. Nuclear staining: DAPI.
Confocal image showing cytoplasmic staining in AtT-20 cell line