Anti-POMC antibody [EPR22534-165] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (AB255334)

Immunohistochemical analysis of paraffin-embedded human pituitary tissue labeling POMC with ab254257 at 1/4000 dilution at room temperature for 30 mins, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pituitary is observed (PMID : 21622576). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was performed. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (AB255334)

Negative control : No staining on mouse spleen (PMID : 10395956)

Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling POMC with ab254257 at 1/4000 dilution at room temperature for 30 mins, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was performed. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (AB255334)

Immunohistochemical analysis of paraffin-embedded rat pituitary tissue labeling POMC with ab254257 at 1/4000 dilution at room temperature for 30 mins, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat pituitary is observed (PMID : 21622576). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was performed. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (AB255334)

Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labelling POMC with ab254257 at 1/4000 dilution at room temperature for 30 mins, followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse pituitary is observed (PMID : 21622576). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was performed. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (AB255334)

Immunohistochemical analysis of 0.2% Triton X-100 permeabilized, 4% PFA fixed rat pituitary tissue labeling POMC with ab254257 at 1/500 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. Positive staining on rat pituitary (PMID : 18601691).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (AB255334)

Negative control : No stain on liver (PMID : 14578285)

Immunohistochemical analysis of 0.2% Triton X-100 permeabilized, 4% PFA fixed mouse liver tissue labeling POMC with ab254257 at 1/100 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. No staining on mouse liver.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (AB255334)

Immunohistochemical analysis of 0.2% Triton X-100 permeabilized, 4% PFA fixed mouse pituitary tissue labeling POMC with ab254257 at 1/500 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. Positive staining on mouse pituitary (PMID : 18601691).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-POMC antibody [EPR22534-165] - BSA and Azide free (AB255334)

Negative control : No stain on liver (PMID : 14578285)

Immunohistochemical analysis of 0.2% Triton X-100 permeabilized, 4% PFA fixed rat liver tissue labeling POMC with ab254257 at 1/100 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed. No staining on rat liver.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254257).