AB92466

Anti-PON1 antibody [EPR2892]

BOND RX™ Validated
RabMAb
Recombinant
What is this?

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(8 Publications)

Rabbit Recombinant Monoclonal PON1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 8 publications.

View Alternative Names

PON, PON1, Serum paraoxonase/arylesterase 1, PON 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1, A-esterase 1

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

ab92466 at 1/100 dilution staining PON1 in formalin-fixed paraffin-embedded Human liver tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

ab92466 showing negative staining in Normal breast tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

ab92466 showing negative staining in Normal colon tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

ab92466 showing negative staining in Normal brain tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

ab92466 showing negative staining in Skeletal muscle tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Unknown

Western blot - Anti-PON1 antibody [EPR2892] (AB92466)

All lanes:

Western blot - Anti-PON1 antibody [EPR2892] (ab92466) at 1/1000 dilution

Lane 1:

Human plasma cell lysate at 10 µg

Lane 2:

ovarian cancer lysate at 10 µg

Secondary

Lane 1:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Lane 2:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 40 kDa

false

Carrier free

Anti-PON1 antibody [EPR2892] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2892

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Mouse species is recommended based on IHC results, we do not guarantee WB for mouse.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.

See full target information PON1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of cancer research and clinical oncology 151:170 PubMed40389753

2025

Investigating the role of the Pon1-rs854560 (L55M) SNP in colorectal Cancer susceptibility.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Wei,Rong Qin,Liang Yin,Muhammad Asad Iqbal,Zakari Shaibu,Guorui Li,Ting Wu

Cancer medicine 13:e70284 PubMed39540710

2024

Predictive Prognostic Model for Hepatocellular Carcinoma Based on Seven Genes Participating in Arachidonic Acid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Gu,Jing Wang,Jun Guan,Guojun Li,Xiao Ma,Yanli Ren,Shanshan Wu,Chao Chen,Haihong Zhu

Molecular oncology 17:59-81 PubMed36400567

2022

Neoadjuvant chemotherapy is associated with an altered metabolic profile and increased cancer stemness in patients with pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Manoj Amrutkar,Caroline S Verbeke,Anette Vefferstad Finstadsveen,Linda Dorg,Knut Jørgen Labori,Ivar P Gladhaug

Cancer cell international 21:621 PubMed34819088

2021

Identification and clinical validation of EMT-associated prognostic features based on hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dafeng Xu,Yu Wang,Jincai Wu,Shixun Lin,Yonghai Chen,Jinfang Zheng

Molecular therapy. Nucleic acids 26:1303-1317 PubMed34853729

2021

Ubiquitinated ligation protein NEDD4L participates in MiR-30a-5p attenuated atherosclerosis by regulating macrophage polarization and lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Song,Jing-Zhou Li,Yao Wu,Wei-Yin Wu,Yan Wang,Gang Li

Frontiers in oncology 11:651421 PubMed33889548

2021

Analysis of Serum Paraoxonase 1 Using Mass Spectrometry and Lectin Immunoassay in Patients With Alpha-Fetoprotein Negative Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Cao,Zhao Cao,Yuyin Shao,Chao Liu,Guoquan Yan,Xinmin Meng,Lei Zhang,Chen Chen,Guiyue Huang,Hong Shu,Haojie Lu

Clinical proteomics 16:34 PubMed31467500

2019

Potential early clinical stage colorectal cancer diagnosis using a proteomics blood test panel.

Applications

Unspecified application

Species

Unspecified reactive species

Seong Beom Ahn,Samridhi Sharma,Abidali Mohamedali,Sadia Mahboob,William J Redmond,Dana Pascovici,Jemma X Wu,Thiri Zaw,Subash Adhikari,Vineet Vaibhav,Edouard C Nice,Mark S Baker

Journal of translational medicine 16:327 PubMed30477582

2018

Evaluation of the prognostic value of paraoxonase 1 in the recurrence and metastasis of hepatocellular carcinoma and establishment of a liver-specific predictive model of survival.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Yu,Qifeng Ou,Fan Chen,Jiong Bi,Wen Li,Jieyi Ma,Rongchang Wang,Xiaohui Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PON1 antibody [EPR2892]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (AB92466)

Western blot - Anti-PON1 antibody [EPR2892] (AB92466)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (8)

Product promise