Anti-PON1 antibody [EPR2892] (ab92466)

Rabbit Recombinant Monoclonal PON1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 6 publications.

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Images

Western blot - Anti-PON1 antibody [EPR2892] (AB92466), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	WB
Human	Tested	Not recommended	Tested
Mouse	Tested	Not recommended	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100 - 1/250	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info 1/100 - 1/250	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information PON1

Function

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.

Alternative names

PON, PON1, Serum paraoxonase/arylesterase 1, PON 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1, A-esterase 1

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Rabbit Recombinant Monoclonal PON1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 6 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR2892
Purity: Tissue culture supernatant
Specificity: Mouse species is recommended based on IHC results, we do not guarantee WB for mouse.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Storage information: Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Western blot - Anti-PON1 antibody [EPR2892] (ab92466)
All lanes: Western blot - Anti-PON1 antibody [EPR2892] (ab92466) at 1/1000 dilution
Lane 1: Human plasma cell lysate at 10 µg
Lane 2: ovarian cancer lysate at 10 µg
Secondary
Lane 1: HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Lane 2: HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
ab92466, at 1/100 dilution, staining PON1 in formalin-fixed, paraffin-embedded Human liver tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
ab92466 showing negative staining in Skeletal muscle tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
ab92466 showing negative staining in Normal colon tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
ab92466 showing negative staining in Normal breast tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] (ab92466)
ab92466 showing negative staining in Normal brain tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.