Anti-PON2 antibody [EPR15295-58]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PON2 antibody [EPR15295-58] (AB183718)

Immunofluorescent analysis of acetone fixed HeLa cells labeling PON2 using ab183718 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-PON2 antibody [EPR15295-58] (AB183718)

Lysate from HepG2 cells was immunoprecipitated with ab183718 at a 1/50 dilution. For the subsequent blot, ab183718 was used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer : 5% NFDM/TBST. Lane 2 : TBS instead of HepG2 lysates.

All lanes:

Immunoprecipitation - Anti-PON2 antibody [EPR15295-58] (ab183718)

Predicted band size: 39 kDa

false

• WB

Supplier Data

Western blot - Anti-PON2 antibody [EPR15295-58] (AB183718)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-PON2 antibody [EPR15295-58] (ab183718) at 1/5000 dilution

All lanes:

HepG2 whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa

false

• WB

Supplier Data

Western blot - Anti-PON2 antibody [EPR15295-58] (AB183718)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-PON2 antibody [EPR15295-58] (ab183718) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

U87-MG whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa

false

• WB

Supplier Data

Western blot - Anti-PON2 antibody [EPR15295-58] (AB183718)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PON2 antibody [EPR15295-58] (ab183718) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Lane 7:

Rat kidney tissue lysate at 20 µg

Lane 8:

Rat spleen tissue lysate at 20 µg

Lane 9:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 10:

Raw264.7 (Mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 11:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 12:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Exposure time: 30s