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Rabbit Recombinant Monoclonal PON2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-PON2 antibody [EPR15295-58] (AB183718), expandable thumbnail
  • Western blot - Anti-PON2 antibody [EPR15295-58] (AB183718), expandable thumbnail
  • Western blot - Anti-PON2 antibody [EPR15295-58] (AB183718), expandable thumbnail
  • Immunoprecipitation - Anti-PON2 antibody [EPR15295-58] (AB183718), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PON2 antibody [EPR15295-58] (AB183718), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/30 - 1/50
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.

Alternative names

Serum paraoxonase/arylesterase 2, PON 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2, A-esterase 2, PON2

Recommended products

Rabbit Recombinant Monoclonal PON2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15295-58
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PON2 antibody [EPR15295-58] (ab183718), expandable thumbnail

    Western blot - Anti-PON2 antibody [EPR15295-58] (ab183718)

    PON2 Western blot staining using rabbit Anti-PON2 antibody

    All lanes: Western blot - Anti-PON2 antibody [EPR15295-58] (ab183718) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Mouse kidney tissue lysate at 20 µg

    Lane 4: Mouse spleen tissue lysate at 20 µg

    Lane 5: Rat brain tissue lysate at 20 µg

    Lane 6: Rat heart tissue lysate at 20 µg

    Lane 7: Rat kidney tissue lysate at 20 µg

    Lane 8: Rat spleen tissue lysate at 20 µg

    Lane 9: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 10: Raw264.7 (Mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 11: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Lane 12: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa

    Observed band size: 39 kDa

    Exposure time: 30s

  • Western blot - Anti-PON2 antibody [EPR15295-58] (ab183718), expandable thumbnail

    Western blot - Anti-PON2 antibody [EPR15295-58] (ab183718)

    Blocking/ Dilution buffer: 5% NFDM /TBST.

    All lanes: Western blot - Anti-PON2 antibody [EPR15295-58] (ab183718) at 1/1000 dilution

    Lane 1: HeLa whole cell lysate at 20 µg

    Lane 2: U87-MG whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Western blot - Anti-PON2 antibody [EPR15295-58] (ab183718), expandable thumbnail

    Western blot - Anti-PON2 antibody [EPR15295-58] (ab183718)

    Blocking/ Dilution buffer: 5% NFDM /TBST.

    All lanes: Western blot - Anti-PON2 antibody [EPR15295-58] (ab183718) at 1/5000 dilution

    All lanes: HepG2 whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Immunoprecipitation - Anti-PON2 antibody [EPR15295-58] (ab183718), expandable thumbnail

    Immunoprecipitation - Anti-PON2 antibody [EPR15295-58] (ab183718)

    Lysate from HepG2 cells was immunoprecipitated with ab183718 at a 1/50 dilution. For the subsequent blot, ab183718 was used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST. Lane 2: TBS instead of HepG2 lysates.

    All lanes: Immunoprecipitation - Anti-PON2 antibody [EPR15295-58] (ab183718)

    Predicted band size: 39 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PON2 antibody [EPR15295-58] (ab183718), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PON2 antibody [EPR15295-58] (ab183718)

    Immunofluorescent analysis of acetone fixed HeLa cells labeling PON2 using ab183718 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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