Rabbit Recombinant Monoclonal PON2 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 7 publications.
Images
![Western blot - Anti-PON2 antibody [EPR15295-82] (AB183710), expandable thumbnail](https://content.abcam.com/products/images/pon2-antibody-epr15295-82-ab183710--western-blot-img2245.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PON2 antibody [EPR15295-82] (AB183710)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (AB183710), expandable thumbnail](https://content.abcam.com/products/images/pon2-antibody-epr15295-82-ab183710--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23605.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (AB183710)")
![Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (AB183710), expandable thumbnail](https://content.abcam.com/products/images/pon2-antibody-epr15295-82-ab183710--immunoprecipitation-img69084.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (AB183710)")
![Western blot - Anti-PON2 antibody [EPR15295-82] (AB183710), expandable thumbnail](https://content.abcam.com/products/images/pon2-antibody-epr15295-82-ab183710--western-blot-img103768.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PON2 antibody [EPR15295-82] (AB183710)")
Publications
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- Biology of sex differences 14:652023Age-associated sex difference in the expression of mitochondria-based redox sensitive proteins and effect of pioglitazone in nonhuman primate brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSumit Jamwal,Jennifer K Blackburn,John D ElsworthPubMed 37770961
- Free radical biology & medicine 167:201-2042021Sex-based disparity in paraoxonase-2 expression in the brains of African green monkeys.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSumit Jamwal,Jennifer K Blackburn,John D ElsworthPubMed 33722626
- Bioscience reports 41:2021Long non-coding RNA LINC00488 facilitates thyroid cancer cell progression through miR-376a-3p/PON2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFuyuan Xie,Longgen Li,Yuting Luo,Rensheng Chen,Jinhong MeiPubMed 33600548
- Journal of cellular physiology 234:3621-36332018Caspase-1-dependent mechanism mediating the harmful impacts of the quorum-sensing molecule N-(3-oxo-dodecanoyl)-l-homoserine lactone on the intestinal cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiyu Tao,Qinwei Sun,Liuping Cai,Yali Geng,Canfeng Hua,Yingdong Ni,Ruqian ZhaoPubMed 30471106
- Free radical biology & medicine 121:117-1262018Paraoxonase 2 prevents the development of heart failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Li et. al.PubMed 29729330
- The Journal of biological chemistry 293:3421-34352018A protein interaction network centered on leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) regulates growth factor receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMahmood Faraz et. al.PubMed 29317492
- The Journal of biological chemistry 292:15927-159382017Regulation of the epithelial Na channel by paraoxonase-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShujie Shi et. al.PubMed 28768768
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/70 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.
Alternative names
Serum paraoxonase/arylesterase 2, PON 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2, A-esterase 2, PON2
Recommended products
Rabbit Recombinant Monoclonal PON2 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15295-82
- Purification technique
- Affinity purification Protein A
- Specificity
Based on the sequence analysis, ab183710 recognizes three isoforms with the predicted MWts of 39KDa, 39KDa and 38KDa, respectively.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710), expandable thumbnail](https://content.abcam.com/products/images/pon2-antibody-epr15295-82-ab183710--western-blot-img2245.jpg "Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)")
Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)
Lanes 1-3: Merged signal (red and green). Green - ab183710 observed at 39,41 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.ab183710 Anti-PON2 antibody [EPR15295-82] was shown to specifically react with PON2 in wild-type HeLa cells. Loss of signal was observed when knockout sample Human PON2 knockout HeLa cell lysate ab258128 was used. Wild-type and PON2 knockout samples were subjected to SDS-PAGE. ab183710 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: PON2 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human PON2 knockout HeLa cell line (Human PON2 knockout HeLa cell line ab265927)
Lane 3: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa, 41 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710), expandable thumbnail](https://content.abcam.com/products/images/pon2-antibody-epr15295-82-ab183710--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23605.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (ab183710)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PON2 with ab183710 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (ab183710), expandable thumbnail](https://content.abcam.com/products/images/pon2-antibody-epr15295-82-ab183710--immunoprecipitation-img69084.jpg "Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (ab183710)")
Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (ab183710)
Western blot analysis of PON2 in HepG2 cell lysate immunoprecipitated with ab183710 at 1/50 dilution (Lane 1). Lane 2: TBS instead of HepG2 lysates.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
All lanes: Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (ab183710)
Predicted band size: 39 kDa
![Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710), expandable thumbnail](https://content.abcam.com/products/images/pon2-antibody-epr15295-82-ab183710--western-blot-img103768.jpg "Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)")
Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710)
All lanes: Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710) at 1/20000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: HepG2 cell lysate at 20 µg
Lane 3: A431 cell lysate at 20 µg
Lane 4: U87-MG cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa, 41 kDa
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