Anti-PON2 antibody [EPR15295-82]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON2 antibody [EPR15295-82] (AB183710)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PON2 with ab183710 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (AB183710)

Western blot analysis of PON2 in HepG2 cell lysate immunoprecipitated with ab183710 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of HepG2 lysates.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-PON2 antibody [EPR15295-82] (ab183710)

Predicted band size: 39 kDa

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• WB

Lab

Western blot - Anti-PON2 antibody [EPR15295-82] (AB183710)

Lanes 1-3 : Merged signal (red and green). Green - ab183710 observed at 39,41 kDa. Red - loading control ab7291 observed at 50 kDa.

ab183710 Anti-PON2 antibody [EPR15295-82] was shown to specifically react with PON2 in wild-type HeLa cells. Loss of signal was observed when knockout sample ab258128 was used. Wild-type and PON2 knockout samples were subjected to SDS-PAGE. ab183710 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PON2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PON2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-pon2-knockout-hela-cell-line-ab265927'>ab265927</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa,41 kDa

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• WB

Supplier Data

Western blot - Anti-PON2 antibody [EPR15295-82] (AB183710)

All lanes:

Western blot - Anti-PON2 antibody [EPR15295-82] (ab183710) at 1/20000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

U87-MG cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa,41 kDa

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