Rabbit Recombinant Monoclonal PON2 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Human samples.
Images
![Western blot - Anti-PON2 antibody [EPR15295-82] - BSA and Azide free (AB250686), expandable thumbnail](https://content.abcam.com/products/images/pon2-antibody-epr15295-82-bsa-and-azide-free-ab250686--western-blot-img2245.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PON2 antibody [EPR15295-82] - BSA and Azide free (AB250686)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Expected | Tested | Expected |
| Mouse | Expected | Not recommended | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.
Alternative names
Serum paraoxonase/arylesterase 2, PON 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2, A-esterase 2, PON2
Recommended products
Rabbit Recombinant Monoclonal PON2 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR15295-82
- Purification technique
- Affinity purification Protein A
- Specificity
Based on the sequence analysis, this antibody recognizes three isoforms with the predicted MWts of 39KDa, 39KDa and 38KDa, respectively.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250686 is the carrier-free version of Anti-PON2 antibody [EPR15295-82] ab183710.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-PON2 antibody [EPR15295-82] - BSA and Azide free (ab250686), expandable thumbnail](https://content.abcam.com/products/images/pon2-antibody-epr15295-82-bsa-and-azide-free-ab250686--western-blot-img2245.jpg "Western blot - Anti-PON2 antibody [EPR15295-82] - BSA and Azide free (ab250686)")
Western blot - Anti-PON2 antibody [EPR15295-82] - BSA and Azide free (ab250686)
This data was developed using the same antibody clone in a different buffer formulation (Anti-PON2 antibody [EPR15295-82] ab183710).
Lanes 1-3: Merged signal (red and green). Green - Anti-PON2 antibody [EPR15295-82] ab183710 observed at 39,41 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.
Anti-PON2 antibody [EPR15295-82] ab183710 Anti-PON2 antibody [EPR15295-82] was shown to specifically react with PON2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human PON2 knockout HeLa cell line ab265927 (knockout cell lysate Human PON2 knockout HeLa cell lysate ab258128) was used. Wild-type and PON2 knockout samples were subjected to SDS-PAGE. Anti-PON2 antibody [EPR15295-82] ab183710 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PON2 antibody [EPR15295-82] (Anti-PON2 antibody [EPR15295-82] ab183710) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: PON2 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human PON2 knockout HeLa cell line (Human PON2 knockout HeLa cell line ab265927)
Lane 3: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa, 41 kDa
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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