Anti-PON2 antibody [EPR15295-82] - BSA and Azide free

• WB

Lab

Western blot - Anti-PON2 antibody [EPR15295-82] - BSA and Azide free (AB250686)

This data was developed using the same antibody clone in a different buffer formulation (ab183710).

Lanes 1-3 : Merged signal (red and green). Green - ab183710 observed at 39,41 kDa. Red - loading control ab7291 observed at 50 kDa.

ab183710 Anti-PON2 antibody [EPR15295-82] was shown to specifically react with PON2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265927 (knockout cell lysate ab258128) was used. Wild-type and PON2 knockout samples were subjected to SDS-PAGE. ab183710 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PON2 antibody [EPR15295-82] (<a href='/en-us/products/primary-antibodies/pon2-antibody-epr15295-82-ab183710'>ab183710</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PON2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PON2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-pon2-knockout-hela-cell-line-ab265927'>ab265927</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa,41 kDa

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