Anti-PON2 antibody [EPR18547-2]

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PON2 antibody [EPR18547-2] (AB192038)

Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized L-929 (mouse connective tissue fibroblast) cell line labeling PON2 with ab192038 at 1/600 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

• IP

Supplier Data

Immunoprecipitation - Anti-PON2 antibody [EPR18547-2] (AB192038)

PON2 was immunoprecipitated from 0.35 mg of L-929 (mouse connective tissue fibroblast cell line) whole cell lysate with ab192038 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab192038 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : L-929 whole cell lysate 10 μg (Input).

Lane 2 : ab192038 IP in L-929 whole cell lysate (+).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab192038 in L-929 whole cell lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-PON2 antibody [EPR18547-2] (ab192038)

Predicted band size: 39 kDa,53 kDa,58 kDa

Observed band size: 40 kDa

true

• WB

Supplier Data

Western blot - Anti-PON2 antibody [EPR18547-2] (AB192038)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-PON2 antibody [EPR18547-2] (ab192038) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Lane 3:

L-929 (mouse connective tissue fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 40 kDa

true

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-PON2 antibody [EPR18547-2] (AB192038)

Blocking and dilution buffer : 5% NFDM/TBST

Exposure times.

Lane 1 : 24 seconds

Lanes 2 & 3 : 3 minutes

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.

All lanes:

Western blot - Anti-PON2 antibody [EPR18547-2] (ab192038) at 1/1000 dilution

Lane 1:

Rat liver tissue lysate at 10 µg

Lane 2:

Rat lung tissue lysate at 10 µg

Lane 3:

Rat heart tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 40 kDa

true

• WB

Supplier Data

Western blot - Anti-PON2 antibody [EPR18547-2] (AB192038)

Blocking and dilution buffer : 5% NFDM/TBST

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.

All lanes:

Western blot - Anti-PON2 antibody [EPR18547-2] (ab192038) at 1/1000 dilution

Lane 1:

GST-His-tagged mouse PON1 (aa2-355) recombinant protein, 10 ng

Lane 2:

His-tagged mouse PON2 (aa1-354) recombinant protein , 10 ng

Lane 3:

His-tagged mouse PON3 (aa2-354) recombinant protein, 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

true

Exposure time: 6s