Anti-PON2 antibody [EPR18547-2] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PON2 antibody [EPR18547-2] - BSA and Azide free (AB228134)

Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized L-929 (mouse connective tissue fibroblast) cell line labeling PON2 with ab192038 at 1/600 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192038).

• IP

Supplier Data

Immunoprecipitation - Anti-PON2 antibody [EPR18547-2] - BSA and Azide free (AB228134)

PON2 was immunoprecipitated from 0.35 mg of L-929 (mouse connective tissue fibroblast cell line) whole cell lysate with ab192038 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab192038 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : L-929 whole cell lysate 10 μg (Input).

Lane 2 : ab192038 IP in L-929 whole cell lysate (+).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab192038 in L-929 whole cell lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192038).

All lanes:

Immunoprecipitation - Anti-PON2 antibody [EPR18547-2] (<a href='/en-us/products/primary-antibodies/pon2-antibody-epr18547-2-ab192038'>ab192038</a>)

Predicted band size: 39 kDa,53 kDa,58 kDa

Observed band size: 40 kDa

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