Anti-POR1 antibody [EPR11943] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POR1 antibody [EPR11943] - BSA and Azide free (AB250206)

This data was developed using ab180503, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling POR2 with ab180503 at 1/500 dilution, followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-POR1 antibody [EPR11943] - BSA and Azide free (AB250206)

This data was developed using ab180503, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling POR2 with ab180503 at 1/150 dilution (red), compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/75 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-POR1 antibody [EPR11943] - BSA and Azide free (AB250206)

This data was developed using ab180503, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling POR2 with ab180503 at 1/1000 dilution, followed by Goat anti rabbit IgG (Dylight 555) secondary antibody at 1/250 dilution. Counter stained with DAPI.

• IP

Supplier Data

Immunoprecipitation - Anti-POR1 antibody [EPR11943] - BSA and Azide free (AB250206)

This data was developed using ab180503, the same antibody clone in a different buffer formulation.

Western blot analysis of SH-SY5Y cell lysate (Lane 1) and Negative control (Lane 2) immunoprecipitated with ab180503 at 1/30 dilution. Rabbit TrueBlot® : Anti-Rabbit IgG HRP secondary antibody used at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-POR1 antibody [EPR11943] (<a href='/en-us/products/primary-antibodies/por1-antibody-epr11943-ab180503'>ab180503</a>)

Predicted band size: 38 kDa

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• WB

Supplier Data

Western blot - Anti-POR1 antibody [EPR11943] - BSA and Azide free (AB250206)

This data was developed using ab180503, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-POR1 antibody [EPR11943] (<a href='/en-us/products/primary-antibodies/por1-antibody-epr11943-ab180503'>ab180503</a>) at 1/5000 dilution

Lane 1:

SH-SY5Y cell lysate at 20 µg

Lane 2:

MCF-7 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

• WB

Lab

Western blot - Anti-POR1 antibody [EPR11943] - BSA and Azide free (AB250206)

This data was developed using the same antibody clone in a different buffer formulation (ab180503).

Lanes 1 - 4 : Merged signal (red and green). Green - ab180503 observed at 38 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab180503 was shown to react with POR1 in western blot. The band observed in the knockout lysate lane above 38 kDa is likely to represent an inserted form. This has not been investigated further. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab180503 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-POR1 antibody [EPR11943] (<a href='/en-us/products/primary-antibodies/por1-antibody-epr11943-ab180503'>ab180503</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ARFIP2 CRISPR-Cas9 Edited HeLa cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 38 kDa

Observed band size: 38 kDa

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