Anti-Porcine circovirus 2-Capsid protein antibody [EPR28077-77]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Porcine circovirus 2-Capsid protein antibody [EPR28077-77] (AB308289)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Porcine circovirus 2-Capsid protein with ab308289 at 1/4000 (0.124 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : no staining on human liver. The section was incubated with ab308289 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Porcine circovirus 2-Capsid protein antibody [EPR28077-77] (AB308289)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Porcine circovirus 2-Capsid protein with ab308289 at 1/4000 (0.124 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : no staining on rat liver. The section was incubated with ab308289 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Porcine circovirus 2-Capsid protein antibody [EPR28077-77] (AB308289)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Porcine circovirus 2-Capsid protein with ab308289 at 1/4000 (0.124 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : no staining on mouse liver. The section was incubated with ab308289 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Porcine circovirus 2-Capsid protein antibody [EPR28077-77] (AB308289)

Immunohistochemical analysis of paraffin-embedded cell pellets labeling Porcine circovirus 2-Capsid protein with ab308289 at 1/4000 (0.124 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a PCV2-Cap expression vector containing a His tag, no staining on (B) HEK-293T transfected with empty vector containing a His tag. The section was incubated with ab308289 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Porcine circovirus 2-Capsid protein antibody [EPR28077-77] (AB308289)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling Porcine circovirus 2-Capsid protein with ab308289 at 1/100 dilution (4.945 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing positive staining in HEK-293T cells transfected with a PCV2-Cap expression vector containing a myc tag. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (0.38 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

• WB

Supplier Data

Western blot - Anti-Porcine circovirus 2-Capsid protein antibody [EPR28077-77] (AB308289)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. This antibody does not cross-react with PCV1-Cap. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 180 seconds.

All lanes:

Western blot - Anti-Porcine circovirus 2-Capsid protein antibody [EPR28077-77] (ab308289) at 1/1000 dilution

Lane 1:

293T cells transfected with a PCV2-Cap expression vector containing a His-tag whole cell lysate at 2 µg

Lane 2:

293T cells transfected with a PCV1-Cap expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 28 kDa

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