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Mouse Monoclonal Porcine circovirus type 2 / PCV2 Capsid antibody. Suitable for ICC/IF, WB and reacts with Pig, Transfected cell line samples.

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Images

Western blot - Anti-Porcine circovirus type 2 / PCV2 Capsid antibody [GT972] - BSA and Azide free (AB308410), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Porcine circovirus type 2 / PCV2 Capsid antibody [GT972] - BSA and Azide free (AB308410), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7
Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Pig
Tested
Expected
Transfected cell line
Not recommended
Tested

Tested
Tested

Species
Pig
Dilution info
1/200
Notes

-

Not recommended
Not recommended

Species
Transfected cell line
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Pig
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Alternative names

PCV1, PCV2, Porcine circovirus 1, Porcine circovirus 2

Mouse Monoclonal Porcine circovirus type 2 / PCV2 Capsid antibody. Suitable for ICC/IF, WB and reacts with Pig, Transfected cell line samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Clone number
GT972
Purification technique
Affinity purification Protein G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The porcine circovirus capsid protein also known as the PCV2 capsid is a primary structural protein integral to the assembly of the virus's protective shell. This protein has a molecular mass of approximately 25 kDa. It is expressed in infected host cells where it plays a significant role in the packaging and protection of the viral genome. The capsid protein is essential for the virion's entry into host cells and subsequent infection cycle.

Biological function summary

The porcine circovirus capsid protein enables the virus to persist in swine hosts by facilitating the immune evasion mechanisms. It forms part of the complete virion structure contributing to the virus's stability in the environment and subsequent transmission between animals. The protein does not alone form a complex but interacts closely with viral genomic elements and host cell machinery to carry out its biological role efficiently.

Pathways

The capsid protein of porcine circovirus integrates into viral replication and immune modulation pathways. It interacts with host signaling proteins that regulate antiviral responses potentially interfering with pathways such as the interferon signaling pathway. These interactions help the virus evade host immunity and support the viral lifecycle within infected swine leading to persistent infection.

Associated diseases and disorders

Porcine circovirus capsid protein has direct links to porcine circovirus-associated diseases (PCVAD). These include post-weaning multisystemic wasting syndrome (PMWS) which severely impacts swine health and productivity. The capsid protein interacts with host cellular proteins influencing disease outcomes and pathology. Continued research into capsid-host interactions might offer insights into therapeutic interventions or vaccine development strategies against PCV2-associated diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Porcine circovirus type 2 / PCV2 Capsid antibody [GT972] - BSA and Azide free (ab308410), expandable thumbnail

    Western blot - Anti-Porcine circovirus type 2 / PCV2 Capsid antibody [GT972] - BSA and Azide free (ab308410)

    Non-infected (–) and infected (+) PK-15 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Porcine circovirus type 2 Capsid antibody [GT972] (ab308410) diluted at 1/1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Porcine circovirus type 2 / PCV2 Capsid antibody [GT972] - BSA and Azide free (ab308410) at 15 µg

    Lane 1: Non-infected (–) PK-15 whole cell extracts at 15 µg

    Lane 2: Infected (+) PK-15 whole cell extracts at 15 µg

    Secondary

    All lanes: HRP-conjugated anti-mouse IgG antibody

  • Immunocytochemistry/ Immunofluorescence - Anti-Porcine circovirus type 2 / PCV2 Capsid antibody [GT972] - BSA and Azide free (ab308410), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Porcine circovirus type 2 / PCV2 Capsid antibody [GT972] - BSA and Azide free (ab308410)

    For immunofluorescence analysis, PCV2 non-infected (left) and infected (right) PK-15 cells were fixed in Acetone for 10 minutes and permeabilized for detection of Porcine circovirus type 2 / PCV2 Capsid using ab308410 at 1/200 dilution (green).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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