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AB308410

Anti-Porcine circovirus type 2 / PCV2 Capsid antibody [GT972] - BSA and Azide free

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(1 Publication)

Mouse Recombinant Monoclonal CAPSD antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Porcine circovirus 2 samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Porcine circovirus 2 Cap.

View Alternative Names

ORF2, Cap, Capsid protein

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Porcine circovirus type 2 / PCV2 Capsid antibody [GT972] - BSA and Azide free (AB308410)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Porcine circovirus type 2 / PCV2 Capsid antibody [GT972] - BSA and Azide free (AB308410)

For immunofluorescence analysis, PCV2 non-infected (left) and infected (right) PK-15 cells were fixed in Acetone for 10 minutes and permeabilized for detection of Porcine circovirus type 2 / PCV2 Capsid using ab308410 at 1/200 dilution (green).

Western blot - Anti-Porcine circovirus type 2 / PCV2 Capsid antibody [GT972] - BSA and Azide free (AB308410)
  • WB

Supplier Data

Western blot - Anti-Porcine circovirus type 2 / PCV2 Capsid antibody [GT972] - BSA and Azide free (AB308410)

Non-infected (–) and infected (+) PK-15 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Porcine circovirus type 2 Capsid antibody [GT972] (ab308410) diluted at 1/1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Porcine circovirus type 2 / PCV2 Capsid antibody [GT972] - BSA and Azide free (ab308410) at 1/1000 dilution

Lane 1:

Non-infected (–) PK-15 whole cell extracts at 15 µg

Lane 2:

Infected (+) PK-15 whole cell extracts at 15 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody

Predicted band size: 28 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GT972

Isotype

IgG2a

Carrier free

Yes

Reacts with

Porcine circovirus 2

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Porcine circovirus 2 Cap. The exact immunogen used to generate this antibody is proprietary information.

O56129

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Porcine circovirus 2": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The porcine circovirus capsid protein also known as the PCV2 capsid is a primary structural protein integral to the assembly of the virus's protective shell. This protein has a molecular mass of approximately 25 kDa. It is expressed in infected host cells where it plays a significant role in the packaging and protection of the viral genome. The capsid protein is essential for the virion's entry into host cells and subsequent infection cycle.
Biological function summary

The porcine circovirus capsid protein enables the virus to persist in swine hosts by facilitating the immune evasion mechanisms. It forms part of the complete virion structure contributing to the virus's stability in the environment and subsequent transmission between animals. The protein does not alone form a complex but interacts closely with viral genomic elements and host cell machinery to carry out its biological role efficiently.

Pathways

The capsid protein of porcine circovirus integrates into viral replication and immune modulation pathways. It interacts with host signaling proteins that regulate antiviral responses potentially interfering with pathways such as the interferon signaling pathway. These interactions help the virus evade host immunity and support the viral lifecycle within infected swine leading to persistent infection.

Porcine circovirus capsid protein has direct links to porcine circovirus-associated diseases (PCVAD). These include post-weaning multisystemic wasting syndrome (PMWS) which severely impacts swine health and productivity. The capsid protein interacts with host cellular proteins influencing disease outcomes and pathology. Continued research into capsid-host interactions might offer insights into therapeutic interventions or vaccine development strategies against PCV2-associated diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Self-assembles to form the virion icosahedral capsid with a T=1 symmetry. This very small capsid (17 - 22 nm in diameter) allows the virus to be very stable in the environment and resistant to some disinfectants, including detergents. Essential for the initial attachment to heparan sulfate moieties and chondroitin sulfate B of the host cell surface proteoglycans. After attachment, the virus is internalized in a clathrin-, caveolae- and dynamin-independent, actin and Rho-GTPase-mediated pathway and traffics to the nucleus. The capsid protein binds and transports the viral genome and Rep across the nuclear envelope (By similarity).
See full target information Cap
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Veterinary sciences 12: PubMed40559812

2025

Efficacy of PCV2 Vaccination Under Natural Conditions: A Longitudinal Study Using PCR and Virus Isolation.

Applications

Unspecified application

Species

Unspecified reactive species

Eugene Mazimpaka,Rissar Siringo Ringo,Tasuku Hirooka,Tamaki Okabayashi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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