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AB201793

Anti-PORCN/PPN antibody [EPR19741]

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(3 Publications)

Rabbit Recombinant Monoclonal PORCN/PPN antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

MG61, PORC, PPN, PORCN, Protein-serine O-palmitoleoyltransferase porcupine, Protein MG61

1 Images
Western blot - Anti-PORCN/PPN antibody [EPR19741] (AB201793)
  • WB

Supplier Data

Western blot - Anti-PORCN/PPN antibody [EPR19741] (AB201793)

Blocking/Dilution buffer : 5% NFDM/TBST.

Our immunogen covers all 5 isoforms but only isoform 5 (42kDa) is observed in our testing condition. This is consistent with the literature (PMID : 21768372).

All lanes:

Western blot - Anti-PORCN/PPN antibody [EPR19741] (ab201793) at 1/1000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 3:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 52 kDa

Observed band size: 42 kDa

true

Exposure time: 3min

  • Carrier free

    Anti-PORCN/PPN antibody [EPR19741] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19741

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

AB64636

Anti-Frizzled 7 antibody

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  • 4
  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein-serine O-palmitoleoyltransferase that acts as a key regulator of the Wnt signaling pathway by mediating the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16 : 1(9Z)), to Wnt proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
See full target information PORCN
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2025:3585885 PubMed40726812

2025

G Protein-Coupled Receptor 30 Attenuates Neuronal Pyroptosis Induced by Subarachnoid Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Peng,Xiqi Hu,Jun He,Ying Xia

Translational cancer research 12:1703-1714 PubMed37588740

2023

PORCN promotes hepatocellular carcinogenesis via Wnt/β-catenin-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Shuzhen Wu,Zhaoxiu Liu,Jing Chen,Tao Ma,Liyang Wang,Tianxin Huang,Yu Sheng,Wei Huang,Cuihua Lu

Nature cell biology 23:953-966 PubMed34475534

2021

Release of Notch activity coordinated by IL-1β signalling confers differentiation plasticity of airway progenitors via Fosl2 during alveolar regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jinwook Choi,Yu Jin Jang,Catherine Dabrowska,Elhadi Iich,Kelly V Evans,Helen Hall,Sam M Janes,Benjamin D Simons,Bon-Kyoung Koo,Jonghwan Kim,Joo-Hyeon Lee
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com