AB327159

Anti-POU3F3 Antibody [CL15302]

Name: Anti-POU3F3 Antibody [CL15302] (ab327159) | Abcam
Brand: Abcam Limited
SKU: AB327159
Price: 600 USD
Availability: InStock

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Mouse Monoclonal POU3F3/Brain1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human POU3F3.

View Alternative Names

BRN1, OTF8, POU3F3, Brain-specific homeobox/POU domain protein 1, Octamer-binding protein 8, Octamer-binding transcription factor 8, Brain-1, Brn-1, Oct-8, OTF-8

11 Images

Immunocytochemistry/ Immunofluorescence - Anti-POU3F3 Antibody [CL15302] (AB327159)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunofluorescence staining in SH-SY5Y cell line using ab327159 at 2–10 µg/ml, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of human kidney using ab327159 at 1 : 1000–1 : 2500 dilution, showing moderate to strong nuclear positivity in cells in tubules.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of human cerebral cortex using ab327159 at 1 : 1000–1 : 2500 dilution, showing moderate to strong nuclear positivity in neurons and glial cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using ab327159 at 1 : 1000–1 : 2500 dilution. Corresponding POU3F3 RNA-seq data are presented for the same tissues.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of rat cerebellum using ab327159 at 1 : 1000–1 : 2500 dilution, showing moderate to strong nuclear positivity in a subset of neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of mouse cerebral cortex using ab327159 at 1 : 1000–1 : 2500 dilution, showing strong nuclear positivity in neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of rat hippocampus using ab327159 at 1 : 1000–1 : 2500 dilution, showing moderate to strong nuclear positivity in a subset of neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of mouse cerebellum using ab327159 at 1 : 1000–1 : 2500 dilution, showing strong nuclear positivity in a subset of neurons.

Supplier Data

Western blot - Anti-POU3F3 Antibody [CL15302] (AB327159)

Western blot analysis in human cell line HEK 293 using ab327159 at 1 µg/ml.

All lanes:

Western blot - Anti-POU3F3 Antibody [CL15302] (ab327159)

All lanes:

Human cell line HEK 293

Predicted band size: 50.33 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of human liver using ab327159 at 1 : 1000–1 : 2500 dilution, showing no positivity in hepatocytes as expected.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15302] (AB327159)

Immunohistochemical staining of human skeletal muscle using ab327159 at 1 : 1000–1 : 2500 dilution, showing no positivity in myocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL15302

Isotype

IgG1

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human POU3F3.

P20264

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development (PubMed : 31303265). Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3') (By similarity).

See full target information POU3F3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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